{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:50:39Z","timestamp":1792785039078,"version":"4.5.0"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:00:00Z","timestamp":1770508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":19,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["NSFC 82200177"],"award-info":[{"award-number":["NSFC 82200177"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81970163"],"award-info":[{"award-number":["81970163"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The National Key Research and Development Program of China","award":["no. 2022YFC2502700"],"award-info":[{"award-number":["no. 2022YFC2502700"]}]},{"name":"Jiangsu Key project","award":["BE2021654"],"award-info":[{"award-number":["BE2021654"]}]},{"DOI":"10.13039\/501100001809","name":"The National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82170218"],"award-info":[{"award-number":["82170218"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Suzhou Projects","award":["GSWS2020039"],"award-info":[{"award-number":["GSWS2020039"]}]},{"name":"Suzhou Municipal Key Laboratory","award":["SZS201615"],"award-info":[{"award-number":["SZS201615"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-026-39353-2","type":"journal-article","created":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T11:53:57Z","timestamp":1770551637000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A novel human acute myeloid leukemia cell line SDEY-AML1 with KMT2A: MLLT3, IKZF1: EVX1 fusions exhibits high tumorigenicity in NSG mice"],"prefix":"10.1038","volume":"16","author":[{"given":"Chenwei","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiheng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoyan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,8]]},"reference":[{"key":"39353_CR1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n2026","author":"LF Newell","year":"2021","unstructured":"Newell, L. F. & Cook, R. J. Advances in acute myeloid leukemia. Bmj https:\/\/doi.org\/10.1136\/bmj.n2026 (2021).","journal-title":"Bmj"},{"issue":"1","key":"39353_CR2","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1182\/blood-2015-07-604538","volume":"127","author":"EM Stein","year":"2016","unstructured":"Stein, E. M. & Tallman, M. S. Emerging therapeutic drugs for AML. Blood. 127(1), 71\u20138. https:\/\/doi.org\/10.1182\/blood-2015-07-604538 (2016).","journal-title":"Blood."},{"key":"39353_CR3","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-023-01424-6","author":"RS Bhansali","year":"2023","unstructured":"Bhansali, R. S., Pratz, K. W. & Lai, C. Recent advances in targeted therapies in acute myeloid leukemia. J. Hematol. Oncol. https:\/\/doi.org\/10.1186\/s13045-023-01424-6 (2023).","journal-title":"J. Hematol. Oncol."},{"issue":"22","key":"39353_CR4","doi-asserted-by":"publisher","first-page":"2349","DOI":"10.1001\/jama.2009.813","volume":"301","author":"J Koreth","year":"2009","unstructured":"Koreth, J. et al. Allogeneic stem cell transplantation for acute myeloid leukemia in first complete remission: systematic review and meta-analysis of prospective clinical trials. Jama. 301(22), 2349\u201361. https:\/\/doi.org\/10.1001\/jama.2009.813 (2009).","journal-title":"Jama."},{"issue":"1","key":"39353_CR5","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.ccell.2016.05.011","volume":"30","author":"V Stavropoulou","year":"2016","unstructured":"Stavropoulou, V. et al. MLL-AF9 expression in hematopoietic stem cells drives a highly invasive AML expressing EMT-related genes linked to poor outcome. Cancer Cell. 30(1), 43\u201358. https:\/\/doi.org\/10.1016\/j.ccell.2016.05.011 (2016).","journal-title":"Cancer Cell."},{"issue":"17","key":"39353_CR6","doi-asserted-by":"publisher","first-page":"3642","DOI":"10.1182\/blood-2010-01-264564","volume":"115","author":"AR Kumar","year":"2010","unstructured":"Kumar, A. R., Sarver, A. L., Wu, B. & Kersey, J. H. Meis1 maintains stemness signature in MLL-AF9 leukemia. Blood. 115(17), 3642\u20133. https:\/\/doi.org\/10.1182\/blood-2010-01-264564 (2010).","journal-title":"Blood."},{"issue":"1","key":"39353_CR7","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1038\/nsmb.1714","volume":"17","author":"T Cierpicki","year":"2010","unstructured":"Cierpicki, T. et al. Structure of the MLL CXXC domain-DNA complex and its functional role in MLL-AF9 leukemia. Nat. Struct. Mol. Biol. 17(1), 62\u20138. https:\/\/doi.org\/10.1038\/nsmb.1714 (2010).","journal-title":"Nat. Struct. Mol. Biol."},{"key":"39353_CR8","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-13-27","author":"KK Fleischmann","year":"2014","unstructured":"Fleischmann, K. K., Pagel, P., Schmid, I. & Roscher, A. A. RNAi-mediated silencing of MLL-AF9 reveals leukemia-associated downstream targets and processes. Mol. Cancer https:\/\/doi.org\/10.1186\/1476-4598-13-27 (2014).","journal-title":"Mol. Cancer"},{"key":"39353_CR9","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-023-01480-y","author":"W Bahaj","year":"2023","unstructured":"Bahaj, W. et al. Novel scheme for defining the clinical implications of TP53 mutations in myeloid neoplasia. J. Hematol. Oncol. https:\/\/doi.org\/10.1186\/s13045-023-01480-y (2023).","journal-title":"J. Hematol. Oncol."},{"issue":"13","key":"39353_CR10","doi-asserted-by":"publisher","first-page":"2952","DOI":"10.1182\/bloodadvances.2022009100","volume":"7","author":"A Stengel","year":"2023","unstructured":"Stengel, A. et al. Specific subtype distribution with impact on prognosis of TP53 single-hit and double-hit events in AML and MDS. Blood Adv. 7(13), 2952\u20132956. https:\/\/doi.org\/10.1182\/bloodadvances.2022009100 (2023).","journal-title":"Blood Adv."},{"issue":"12","key":"39353_CR11","doi-asserted-by":"publisher","first-page":"2395","DOI":"10.1038\/s41375-023-02061-1","volume":"37","author":"JN Eckardt","year":"2023","unstructured":"Eckardt, J. N. et al. Mutated IKZF1 is an independent marker of adverse risk in acute myeloid leukemia. Leukemia. 37(12), 2395\u20132403. https:\/\/doi.org\/10.1038\/s41375-023-02061-1 (2023).","journal-title":"Leukemia."},{"issue":"6","key":"39353_CR12","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1309","volume":"13","author":"Y Wang","year":"2023","unstructured":"Wang, Y. et al. Identification of IKZF1 genetic mutations as new molecular subtypes in acute myeloid leukaemia. Clin. Transl. Med. 13(6), e1309. https:\/\/doi.org\/10.1002\/ctm2.1309 (2023).","journal-title":"Clin. Transl. Med."},{"issue":"1","key":"39353_CR13","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.cancergencyto.2005.03.004","volume":"162","author":"B Poppe","year":"2005","unstructured":"Poppe, B. et al. HOXA gene cluster rearrangement in a t(7;9)(p15;q34) in a child with MDS. Cancer Genet. Cytogenet. 162(1), 82\u20134. https:\/\/doi.org\/10.1016\/j.cancergencyto.2005.03.004 (2005).","journal-title":"Cancer Genet. Cytogenet."},{"issue":"4","key":"39353_CR14","doi-asserted-by":"publisher","first-page":"508","DOI":"10.1007\/s12185-023-03619-6","volume":"118","author":"X Zhang","year":"2023","unstructured":"Zhang, X. et al. Transcriptome sequencing identifies novel EVX fusions involved in transcriptional activation of HOX family genes in pediatric immature T-cell acute lymphoblastic leukemia: Two cases reports and a literature review. Int. J. Hematol. 118(4), 508\u2013513. https:\/\/doi.org\/10.1007\/s12185-023-03619-6 (2023).","journal-title":"Int. J. Hematol."},{"issue":"5","key":"39353_CR15","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1002\/hon.2686","volume":"37","author":"Z Wang","year":"2019","unstructured":"Wang, Z. et al. Establishment and characterization of a DOT1L inhibitor-sensitive human acute monocytic leukemia cell line YBT-5 with a novel KMT2A-MLLT3 fusion. Hematol. Oncol. 37(5), 617\u2013625. https:\/\/doi.org\/10.1002\/hon.2686 (2019).","journal-title":"Hematol. Oncol."},{"issue":"2","key":"39353_CR16","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1038\/sj.leu.2403236","volume":"18","author":"HG Drexler","year":"2004","unstructured":"Drexler, H. G., Quentmeier, H. & MacLeod, R. A. Malignant hematopoietic cell lines: In vitro models for the study of MLL gene alterations. Leukemia. 18(2), 227\u201332. https:\/\/doi.org\/10.1038\/sj.leu.2403236 (2004).","journal-title":"Leukemia."},{"issue":"6","key":"39353_CR17","first-page":"766","volume":"90","author":"S Chen","year":"2005","unstructured":"Chen, S. et al. A new human acute monocytic leukemia cell line SHI-1 with t(6;11)(q27;q23), p53 gene alterations and high tumorigenicity in nude mice. Haematologica. 90(6), 766\u201375 (2005).","journal-title":"Haematologica."},{"issue":"42","key":"39353_CR18","doi-asserted-by":"publisher","first-page":"26340","DOI":"10.1073\/pnas.2014732117","volume":"117","author":"M Bill","year":"2020","unstructured":"Bill, M. et al. Mutational landscape and clinical outcome of patients with de novo acute myeloid leukemia and rearrangements involving 11q23\/KMT2A. Proc. Natl. Acad. Sci. U S A. 117(42), 26340\u201326346. https:\/\/doi.org\/10.1073\/pnas.2014732117 (2020).","journal-title":"Proc. Natl. Acad. Sci. U S A."},{"issue":"12","key":"39353_CR19","doi-asserted-by":"publisher","first-page":"3200","DOI":"10.1182\/bloodadvances.2023011771","volume":"8","author":"RE van Weelderen","year":"2024","unstructured":"van Weelderen, R. E. et al. Optimized cytogenetic risk-group stratification of KMT2A-rearranged pediatric acute myeloid leukemia. Blood Adv. 8(12), 3200\u20133213. https:\/\/doi.org\/10.1182\/bloodadvances.2023011771 (2024).","journal-title":"Blood Adv."},{"key":"39353_CR20","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-01288-1","author":"P Casado","year":"2023","unstructured":"Casado, P. et al. Integrative phosphoproteomics defines two biologically distinct groups of KMT2A rearranged acute myeloid leukaemia with different drug response phenotypes. Signal Transduct. Target Ther. https:\/\/doi.org\/10.1038\/s41392-022-01288-1 (2023).","journal-title":"Signal Transduct. Target Ther."},{"issue":"8","key":"39353_CR21","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1364","volume":"13","author":"N Osada","year":"2023","unstructured":"Osada, N. et al. c-FOS is an integral component of the IKZF1 transactivator complex and mediates lenalidomide resistance in multiple myeloma. Clin. Transl. Med. 13(8), e1364. https:\/\/doi.org\/10.1002\/ctm2.1364 (2023).","journal-title":"Clin. Transl. Med."},{"issue":"13","key":"39353_CR22","doi-asserted-by":"publisher","first-page":"2024","DOI":"10.1182\/blood.2021014701","volume":"139","author":"W Wu","year":"2022","unstructured":"Wu, W. et al. Overcoming IMiD resistance in T-cell lymphomas through potent degradation of ZFP91 and IKZF1. Blood. 139(13), 2024\u20132037. https:\/\/doi.org\/10.1182\/blood.2021014701 (2022).","journal-title":"Blood."},{"key":"39353_CR23","doi-asserted-by":"crossref","unstructured":"Paolino, J., Tsai, H.K., Harris, M.H., Pikman, Y. IKZF1 alterations and therapeutic targeting in B-Cell acute lymphoblastic leukemia. Biomedicines 12(1) 10.3390\/ biomedicines12010089 (2024).","DOI":"10.3390\/biomedicines12010089"},{"key":"39353_CR24","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.826245","volume":"11","author":"F Nguyen-Khac","year":"2021","unstructured":"Nguyen-Khac, F. \u201cDouble-Hit\u201d chronic lymphocytic leukemia, involving the TP53 and MYC genes. Front. Oncol. 11, 826245. https:\/\/doi.org\/10.3389\/fonc.2021.826245 (2021).","journal-title":"Front. Oncol."},{"issue":"1","key":"39353_CR25","doi-asserted-by":"publisher","DOI":"10.1002\/hon.3238","volume":"42","author":"S Molica","year":"2024","unstructured":"Molica, S., Tam, C., Allsup, D. & Polliack, A. Targeting TP53 disruption in chronic lymphocytic leukemia: Current strategies and future directions. Hematol. Oncol. 42(1), e3238. https:\/\/doi.org\/10.1002\/hon.3238 (2024).","journal-title":"Hematol. Oncol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-39353-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-39353-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-39353-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T00:01:43Z","timestamp":1772236903000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-39353-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,8]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["39353"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-39353-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,8]]},"assertion":[{"value":"2 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 February 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Patient: This study was approved by the Ethics Committee of the Children\u2019s Hospital of Soochow University (No. 2017047-3) and was in accordance with Declaration of Helsinki. All research was performed in accordance with relevant guidelines, Informed Consent was obtained from all subjects and\/or their legal guardian(s). Animal: All animal experiments were conducted in compliance with the animal care guidelines approved by the Institutional Laboratory Animal Care and Use Committee of Soochow University (SUDA20230802A05) and were in accordance with the ARRIVE guidelines.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics"}},{"value":"Consent for publication was obtained from the participants.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"7792"}}