{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:42:05Z","timestamp":1791646925640,"version":"4.3.4"},"reference-count":60,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":31,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Multidrug-resistant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    represents a growing public health challenge in low-resource settings, where therapeutic options and routine genomic surveillance remain limited. Here, we present a structural bioinformatics analysis of resistance-associated missense mutations identified in Sudanese\n                    <jats:italic>E.coli<\/jats:italic>\n                    clinical isolates. Using whole-genome sequencing data from 55 isolates, we performed an integrated in silico analysis of key antibiotic target proteins to characterize missense variation and assess its potential functional consequences. Consensus pathogenicity prediction identified 19 substitutions as likely deleterious, including multiple novel, previously unreported variants clustered in ribosomal protein L22 (rplV), alongside established resistance-associated substitutions in RNA polymerase and topoisomerase IV. These results expand the mutational landscape of resistance-associated targets in Sudanese strains and highlight region-specific patterns of genetic variation. Structure-based modeling and molecular docking revealed mutation-specific effects on predicted antibiotic interactions across multiple targets, including reduced binding for selected topoisomerase inhibitors and heterogeneous effects on macrolide interactions within L22. In contrast, a gyrase variant retained predicted fluoroquinolone binding despite reduced structural stability, suggesting resistance-associated effects beyond direct drug\u2013target interactions. Collectively, these findings underscore the contribution of non-canonical missense mutations to antimicrobial resistance in Sudanese\n                    <jats:italic>E.coli<\/jats:italic>\n                    and demonstrate the utility of computational prioritization frameworks to support resistance surveillance and guide targeted experimental validation.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-39491-7","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T05:46:05Z","timestamp":1770961565000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Structural modeling and docking analysis of canonical and novel resistance-associated missense mutations in Sudanese Escherichia coli"],"prefix":"10.1038","volume":"16","author":[{"given":"Edison Eukun","family":"Sage","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabah A. 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E.","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moaaz M.","family":"Saadaldin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omnia H.","family":"Suliman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osama","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia B.","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qurashi M.","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"39491_CR1","doi-asserted-by":"publisher","first-page":"1387497","DOI":"10.3389\/fcimb.2024.1387497","volume":"14","author":"S Nasrollahian","year":"2024","unstructured":"Nasrollahian, S., Graham, J. P. & Halaji, M. Mechanisms conferring antibiotic resistance in pathotypes of Escherichia coli. Front. Cell. Infect. Microbiol. 14, 1387497. https:\/\/doi.org\/10.3389\/fcimb.2024.1387497 (2024).","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"39491_CR2","unstructured":"World Health Organization. Antimicrobial Resistance (WHO Fact Sheet, 2023)."},{"key":"39491_CR3","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1186\/s12879-025-11437-w","volume":"25","author":"AIA Ibrahim","year":"2025","unstructured":"Ibrahim, A. I. A. et al. Bacterial etiology of urinary tract infections and antimicrobial sensitivity patterns in Port Sudan City, sudan: a retrospective study. BMC Infect. Dis. 25, 1017. https:\/\/doi.org\/10.1186\/s12879-025-11437-w (2025).","journal-title":"BMC Infect. Dis."},{"key":"39491_CR4","doi-asserted-by":"publisher","first-page":"56","DOI":"10.18502\/sjms.v17i1.10685","volume":"17","author":"HM Eezzeldin","year":"2022","unstructured":"Eezzeldin, H. M., Badi, S. & Yousef, B. A. Antibiotic resistance and multidrug resistance patterns of uropathogenic Escherichia coli at Soba university hospital. Sudan. J. Med. Sci. 17, 56\u201369 (2022).","journal-title":"Sudan. J. Med. Sci."},{"key":"39491_CR5","doi-asserted-by":"publisher","first-page":"2131","DOI":"10.2147\/IDR.S464501","volume":"17","author":"YB Hamadalneel","year":"2024","unstructured":"Hamadalneel, Y. B. et al. Prevalence and antimicrobial sensitivity patterns of uropathogens in wad Medani, Sudan. Infect. Drug Resist. 17, 2131\u20132140. https:\/\/doi.org\/10.2147\/IDR.S464501 (2024).","journal-title":"Infect. Drug Resist."},{"key":"39491_CR6","doi-asserted-by":"publisher","unstructured":"Poirel, L. et al. Antimicrobial resistance in Escherichia coli. Microbiol. 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