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In this study, a temperature-responsive spin column composed of various poly(\n                    <jats:italic>N<\/jats:italic>\n                    -isopropylacrylamide) (PNIPAAm) copolymer-modified bead-packed layers was developed. The optimal conditions for separating voriconazole, lamotrigine, and carbamazepine from the serum using the prepared columns were determined. Voriconazole was effectively separated using a bead-packed spin column modified with P(NIPAAm-\n                    <jats:italic>co<\/jats:italic>\n                    -\n                    <jats:italic>N<\/jats:italic>\n                    ,\n                    <jats:italic>N<\/jats:italic>\n                    -dimethylacrylamide (DMAPAAm)7.5%-\n                    <jats:italic>co<\/jats:italic>\n                    -\n                    <jats:italic>n<\/jats:italic>\n                    -butyl methacrylate (BMA)0%) as the upper layer, which was attributed to the desorption of voriconazole at low temperatures. A high elution ratio was achieved for lamotrigine using a bead-packed spin column modified with P(NIPAAm-\n                    <jats:italic>co<\/jats:italic>\n                    -DMAPAAm7.5%-\n                    <jats:italic>co<\/jats:italic>\n                    -BMA0%). Conversely, a high carbamazepine elution ratio was obtained using P(NIPAAm-\n                    <jats:italic>co<\/jats:italic>\n                    -DMAPAAm7.5%\u2013\n                    <jats:italic>co<\/jats:italic>\n                    -BMA5%). These findings indicate that the copolymer composition of temperature-responsive spin columns can be tailored for the separation of specific drugs.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-39613-1","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T06:44:20Z","timestamp":1770878660000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization of the composition of temperature-responsive polymers for spin columns"],"prefix":"10.1038","volume":"16","author":[{"given":"Kenichi","family":"Nagase","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matsurika","family":"Kokubun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideko","family":"Kanazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"39613_CR1","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1046\/j.1365-2125.1998.00770.x","volume":"46","author":"AS Gross","year":"1998","unstructured":"Gross, A. 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