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Understanding the molecular basis of antimicrobial resistance mechanisms is crucial in the preservation of antibiotic efficiency. This study aimed to elucidate the genetic background of antibiotic susceptibility profiles of 110\u00a0M\n                    <jats:italic>. anserisalpingitidis<\/jats:italic>\n                    strains against nine antimicrobial agents. Significant associations between k-mers and five (tylvalosin, tilmicosin, enrofloxacin, lincomycin, spectinomycin) of the nine antimicrobial agents were identified by pyseer. Significant associations were found in multiple coding sequences that encode various members of efflux pumps, epigenetic regulation and topoisomerases among many other groups of functions. Certain k-mers associated with genes found putative prophage-like sequences suggest potential horizontal gene transfer events that could facilitate the acquisition of novel resistance mechanisms. Based on our findings, the genetic background of antimicrobial resistance of\n                    <jats:italic>M. anserisalpingitidis<\/jats:italic>\n                    is composed of multiple factors. Our results not only correlated with the majority of known antibiotic resistance mechanisms (e.g. drug target modification, efflux pumps, methyltransferases) but also showed potentially novel genes that could play a significant role in antimicrobial resistance. The results may serve to expedite the diagnosis of\n                    <jats:italic>M. anserisalpingitidis<\/jats:italic>\n                    antibiotic susceptibility profiles and support the fight against the spreading of resistance.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-39804-w","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T21:14:52Z","timestamp":1771967692000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genome-wide association study of Mycoplasma anserisalpingitidis strains for antibiotic susceptibility"],"prefix":"10.1038","volume":"16","author":[{"given":"\u00c1ron B.","family":"Kov\u00e1cs","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enik\u0151","family":"Wehmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katinka","family":"Bek\u0151","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e9nes","family":"Gr\u00f3zner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krisztina","family":"Bali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsuzsa","family":"Kreizinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sawicka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kriszti\u00e1n","family":"B\u00e1nyai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikl\u00f3s","family":"Gyuranecz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"39804_CR1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004052","author":"DV Volokhov","year":"2020","unstructured":"Volokhov, D. 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