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However, the molecular mechanism underlying periodontal inflammation caused by\n                    <jats:italic>VPS13B<\/jats:italic>\n                    dysfunction in human gingival epithelial cells remains unclear. A previous report noted that coxsackievirus and adenovirus receptor (CXADR) and junctional adhesion molecule 1 (JAM1) are involved in barrier functions against penetration by lipopolysaccharide (LPS) and peptidoglycan (PGN) into gingival tissues. The present study was conducted to examine the effects and significance of\n                    <jats:italic>VPS13B<\/jats:italic>\n                    on gingival barrier function. It was confirmed that loss of VPS13B resulted in decreased cell surface localization of CXADR, but not of JAM1. Additionally, abundant lysosomal localization of CXADR was detected in\n                    <jats:italic>VPS13B<\/jats:italic>\n                    -knockout cells followed treatment with bafilomycin A1, an inhibitor of lysosomal degradation. Other findings indicated that cell-surface localization of the CXADR-chimeric protein, in which C-terminus was exchanged with JAM1, was not disturbed by\n                    <jats:italic>VPS13B<\/jats:italic>\n                    knockout. Finally,\n                    <jats:italic>VPS13B<\/jats:italic>\n                    knockout led to greater permeability of gingival epithelial cell layers and tissues to LPS and PGN, which was restored by increased expression of CXADR-JAM1\n                    <jats:sup>c\u2212term<\/jats:sup>\n                    . Together, these results show that VPS13B is involved in intracellular trafficking of CXADR as well as the barrier function of human gingival epithelial tissues, and thus indicate the molecular basis for periodontal complications in patients affected by Cohen syndrome.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-40840-9","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T02:16:02Z","timestamp":1771899362000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["VPS13B, gene responsible for Cohen syndrome, regulates gingival epithelial barrier function via intracellular trafficking of coxsackievirus and adenovirus receptor"],"prefix":"10.1038","volume":"16","author":[{"given":"Risako","family":"Matsumura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Tanigaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsukasa","family":"Tamamori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Yamaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akito","family":"Sakanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuo","family":"Amano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiya","family":"Matsusaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masae","family":"Kuboniwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"40840_CR1","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1016\/S0022-3476(73)80493-7","volume":"83","author":"MM Cohen","year":"1973","unstructured":"Cohen, M. 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