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This study investigates the antimicrobial properties of selected peptides derived from the Black Soldier Fly (BSF), identified\n                    <jats:italic>in silico<\/jats:italic>\n                    . Ten synthesized peptides were evaluated\n                    <jats:italic>in vitro<\/jats:italic>\n                    against Gram-positive bacteria (\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Bacillus cereus<\/jats:italic>\n                    ), Gram-negative bacteria (\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ), and fungi (\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    ,\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    ). Cytotoxicity was assessed using human lung fibroblasts and red blood cells. Among the peptides, Hill_BB_C7176 demonstrated broad-spectrum antibacterial activity, no cytotoxicity and low hemolytic activity (IC\u2085\u2080: 31.6 - &gt;32 \u00b5M). The peptide displayed rapid bactericidal activity achieving complete eradication at 8\u00d7\u00a0its minimal inhibitory concentration. Propidium iodide uptake increased in a concentration dependent manner, indicating membrane permeabilization contributing to antibacterial activity. In addition, Hill_BB_C7176 exhibited strong binding affinity to lipopolysaccharide (lipid A).\n                    <jats:italic>In vivo<\/jats:italic>\n                    , Hill_BB_C7176 improved survival of\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    larvae infected with\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and\n                    <jats:italic>E. coli<\/jats:italic>\n                    , showing similar survival outcomes to reference antibiotics under the experimental conditions tested. These findings highlight the antibacterial potential of BSF-derived antimicrobial peptides, particularly Hill_BB_C7176, supporting the further research on the antibacterial activity of BSF antimicrobial peptides.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-40997-3","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T17:24:09Z","timestamp":1771953849000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antimicrobial effects of novel Hermetia illucens peptides"],"prefix":"10.1038","volume":"16","author":[{"given":"Emine","family":"Derin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Van Moll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milan","family":"Wouters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"De Vooght","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"De Stefano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Scieuzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Cos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Falabella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"40997_CR1","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","volume":"399","author":"CJ Murray","year":"2022","unstructured":"Murray, C. 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