{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T13:56:01Z","timestamp":1787752561384,"version":"build-2784847793"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":42,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI155472"],"award-info":[{"award-number":["R01AI155472"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI155472"],"award-info":[{"award-number":["R01AI155472"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-026-42373-7","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T09:57:24Z","timestamp":1773136644000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluating the detection capabilities of nanopore sequencing for Borrelia burgdorferi detection in blacklegged ticks"],"prefix":"10.1038","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3322-6319","authenticated-orcid":false,"given":"Jacob","family":"Cassens","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5153-6467","authenticated-orcid":false,"given":"Evan J.","family":"Kipp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7736-4146","authenticated-orcid":false,"given":"Lexi E.","family":"Frank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3634-3625","authenticated-orcid":false,"given":"Peter A.","family":"Larsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan D.","family":"Oliver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"issue":"4","key":"42373_CR1","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1016\/j.pt.2017.12.006","volume":"34","author":"RJ Eisen","year":"2018","unstructured":"Eisen, R. J. & Eisen, L. The blacklegged tick, Ixodes scapularis: An increasing public health concern. Trends Parasitol. 34(4), 295\u2013309 (2018).","journal-title":"Trends Parasitol."},{"issue":"3","key":"42373_CR2","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1093\/ilar\/ilx005","volume":"58","author":"RJ Eisen","year":"2017","unstructured":"Eisen, R. J., Kugeler, K. J., Eisen, L., Beard, C. B. & Paddock, C. D. Tick-Borne Zoonoses in the United States: Persistent and Emerging Threats to Human Health. ILAR J. 58 (3), 319\u2013335 (2017).","journal-title":"ILAR J."},{"issue":"2","key":"42373_CR3","doi-asserted-by":"publisher","first-page":"616","DOI":"10.3201\/eid2702.202731","volume":"27","author":"KJ Kugeler","year":"2021","unstructured":"Kugeler, K. J., Schwartz, A. M., Delorey, M. J., Mead, P. S. & Hinckley, A. F. Estimating the frequency of Lyme disease diagnoses, United States, 2010\u20132018. Emerg. Infect. Dis. 27(2), 616\u2013619 (2021).","journal-title":"Emerg. Infect. Dis."},{"issue":"6","key":"42373_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2023.102233","volume":"14","author":"L Eisen","year":"2023","unstructured":"Eisen, L. & Eisen, R. J. Changes in the geographic distribution of the blacklegged tick, Ixodes scapularis, in the United States. Ticks Tick-borne Dis. 14(6), 102233 (2023).","journal-title":"Ticks Tick-borne Dis."},{"key":"42373_CR5","doi-asserted-by":"crossref","unstructured":"Adrion, E. R., Aucott, J., Lemke, K. W. & Weiner, J. P. Health Care Costs, Utilization and Patterns of Care following Lyme Disease. Brissette CA, editor. PLoS ONE. ;10(2):e0116767. (2015).","DOI":"10.1371\/journal.pone.0116767"},{"key":"42373_CR6","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.816547","volume":"11","author":"G Neelakanta","year":"2021","unstructured":"Neelakanta, G. & Sultana, H. Tick saliva and salivary glands: What do we know so far on their role in arthropod blood feeding and pathogen transmission. Front. Cell. Infect. Microbiol. 11, 816547 (2021).","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"42373_CR7","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.epidem.2016.12.002","volume":"19","author":"SL States","year":"2017","unstructured":"States, S. L., Huang, C. I., Davis, S., Tufts, D. M. & Diuk-Wasser, M. A. Co-feeding transmission facilitates strain coexistence in Borrelia burgdorferi, the Lyme disease agent. Epidemics 19, 33\u201342 (2017).","journal-title":"Epidemics"},{"key":"42373_CR8","doi-asserted-by":"publisher","first-page":"1478","DOI":"10.1093\/jme\/tjae113","volume":"14;61(6)","author":"GM Dill","year":"2024","unstructured":"Dill, G. M., Rounsville, T. F., Bryant, A. M., Groden, E. & Gardner, A. M. Effects of Peromyscus spp. (Rodentia: Cricetidae) presence, land use, and ecotone on Ixodes scapularis (Acari: Ixodidae) ecology in an emergent area for tick-borne disease. J. Med. Entomol. 14;61(6), 1478\u201388 (2024).","journal-title":"J. Med. Entomol."},{"issue":"4","key":"42373_CR9","doi-asserted-by":"publisher","first-page":"1546","DOI":"10.1093\/jme\/tjaa209","volume":"58","author":"MA Diuk-Wasser","year":"2021","unstructured":"Diuk-Wasser, M. A., VanAcker, M. C. & Fernandez, M. P. Impact of Land Use Changes and Habitat Fragmentation on the Eco-epidemiology of Tick-Borne Diseases. J. Med. Entomol. 58 (4), 1546\u20131564 (2021).","journal-title":"J. Med. Entomol."},{"issue":"6","key":"42373_CR10","doi-asserted-by":"publisher","first-page":"e0239121","DOI":"10.1128\/aem.02391-21","volume":"88","author":"HK Goethert","year":"2022","unstructured":"Goethert, H. K. & Telford, S. R. Host contributions to the force of Borrelia burgdorferi and Babesia microti transmission differ at edges of and within a small habitat patch. Appl. Environ. Microbiol. 88(6), e0239121 (2022).","journal-title":"Appl. Environ. Microbiol."},{"key":"42373_CR11","doi-asserted-by":"crossref","unstructured":"Tsao, J. I., Hamer, S. A., Han, S., Sidge, J. L. & Hickling, G. J. The Contribution of Wildlife Hosts to the Rise of Ticks and Tick-Borne Diseases in North America. Reisen W, editor. Journal of Medical Entomology. ;58(4):1565\u201387. (2021).","DOI":"10.1093\/jme\/tjab047"},{"issue":"5","key":"42373_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2022.102000","volume":"13","author":"JC Burtis","year":"2022","unstructured":"Burtis, J. C. et al. Predicting distributions of blacklegged ticks (Ixodes scapularis), Lyme disease spirochetes (Borrelia burgdorferi sensu stricto) and human Lyme disease cases in the eastern United States. Ticks and Tick-borne Diseases 13(5), 102000 (2022).","journal-title":"Ticks and Tick-borne Diseases"},{"issue":"2","key":"42373_CR13","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1093\/jme\/tjv199","volume":"53","author":"RJ Eisen","year":"2016","unstructured":"Eisen, R. J., Eisen, L., Ogden, N. H. & Beard, C. B. Linkages of weather and climate with Ixodes scapularis and Ixodes pacificus (Acari: Ixodidae), enzootic transmission of Borrelia burgdorferi, and Lyme disease in North America. J. Med. Entomol. 53(2), 250\u201361 (2016).","journal-title":"J. Med. Entomol."},{"key":"42373_CR14","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2020.2278","volume":"23;287(1941)","author":"AM Gardner","year":"2020","unstructured":"Gardner, A. M. et al. Landscape features predict the current and forecast the future geographic spread of Lyme disease. Proc. R. Soc. B 23;287(1941), 20202278 (2020).","journal-title":"Proc. R. Soc. B"},{"issue":"7\u20138","key":"42373_CR15","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1016\/j.micinf.2017.12.015","volume":"20","author":"A Cabezas-Cruz","year":"2018","unstructured":"Cabezas-Cruz, A., Vayssier-Taussat, M. & Greub, G. Tick-borne pathogen detection: What\u2019s new?. Microbes Infect. 20(7\u20138), 441\u2013444 (2018).","journal-title":"Microbes Infect."},{"key":"42373_CR16","doi-asserted-by":"crossref","unstructured":"Tokarz, R. & Lipkin, W. I. Discovery and Surveillance of Tick-Borne Pathogens. Reisen W, editor. Journal of Medical Entomology. ;58(4):1525\u201335. (2021).","DOI":"10.1093\/jme\/tjaa269"},{"issue":"7","key":"42373_CR17","doi-asserted-by":"publisher","first-page":"388","DOI":"10.3390\/v10070388","volume":"10","author":"ST Cross","year":"2018","unstructured":"Cross, S. T. et al. Co-infection patterns in individual Ixodes scapularis ticks reveal associations between viral, eukaryotic and bacterial microorganisms. Viruses 10(7), 388 (2018).","journal-title":"Viruses"},{"issue":"1","key":"42373_CR18","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2020.101607","volume":"12","author":"SJ Cutler","year":"2021","unstructured":"Cutler, S. J. et al. Tick-borne diseases and co-infection: Current considerations. Ticks Tick Borne Dis 12(1), 101607 (2021).","journal-title":"Ticks Tick Borne Dis"},{"issue":"4","key":"42373_CR19","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.316","volume":"4","author":"P Zheng","year":"2023","unstructured":"Zheng, P. et al. Nanopore sequencing technology and its applications. MedComm 4(4), e316 (2023).","journal-title":"MedComm"},{"key":"42373_CR20","doi-asserted-by":"publisher","unstructured":"Martin, S. et al. Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples. Genome Biol [Internet]. 2022 Dec [cited 2025 Jul 23];23(1). Available from: https:\/\/genomebiology.biomedcentral.com\/articles\/https:\/\/doi.org\/10.1186\/s13059-021-02582-x","DOI":"10.1186\/s13059-021-02582-x"},{"key":"42373_CR21","doi-asserted-by":"crossref","unstructured":"Li, H. Minimap2: pairwise alignment for nucleotide sequences. Birol I, editor. Bioinformatics. ;34(18):3094\u2013100. (2018).","DOI":"10.1093\/bioinformatics\/bty191"},{"issue":"1","key":"42373_CR22","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1186\/s13071-023-05679-3","volume":"16","author":"EJ Kipp","year":"2023","unstructured":"Kipp, E. J. et al. Nanopore adaptive sampling for targeted mitochondrial genome sequencing and bloodmeal identification in hematophagous insects. Parasites Vectors. 16 (1), 68 (2023).","journal-title":"Parasites Vectors"},{"issue":"1","key":"42373_CR23","doi-asserted-by":"publisher","first-page":"10991","DOI":"10.1038\/s41598-023-37134-9","volume":"13","author":"EJ Kipp","year":"2023","unstructured":"Kipp, E. J. et al. Metagenomic surveillance for bacterial tick-borne pathogens using nanopore adaptive sampling. Sci. Rep. 13 (1), 10991 (2023).","journal-title":"Sci. Rep."},{"key":"42373_CR24","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2023.101135","volume":"61","author":"JH Badger","year":"2024","unstructured":"Badger, J. H. et al. Direct sequencing of insect symbionts via nanopore adaptive sampling. Curr Opin Insect Sci 61, 101135 (2024).","journal-title":"Curr Opin Insect Sci"},{"key":"42373_CR25","doi-asserted-by":"crossref","unstructured":"Frank, L. E. et al. Rapid molecular species identification of mammalian scat samples using nanopore adaptive sampling. Smyser T, editor. Journal of Mammalogy. ;105(5):965\u201375. (2024).","DOI":"10.1093\/jmammal\/gyae044"},{"key":"42373_CR26","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1330814","volume":"15","author":"FE Buytaers","year":"2024","unstructured":"Buytaers, F. E. et al. Strain-level characterization of foodborne pathogens without culture enrichment for outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling. Front. Microbiol. 15, 1330814 (2024).","journal-title":"Front. Microbiol."},{"key":"42373_CR27","doi-asserted-by":"publisher","first-page":"307","DOI":"10.3389\/fpubh.2017.00307","volume":"5","author":"R Trevethan","year":"2017","unstructured":"Trevethan, R. Sensitivity, Specificity, and Predictive Values: Foundations, Pliabilities, and Pitfalls in Research and Practice. Front. Public. Health. 5, 307 (2017).","journal-title":"Front. Public. Health"},{"issue":"4","key":"42373_CR28","doi-asserted-by":"publisher","first-page":"677","DOI":"10.3201\/eid2304.161716","volume":"23","author":"A Chow","year":"2017","unstructured":"Chow, A., Ho, H., Win, M. K. & Leo, Y. S. Assessing sensitivity and specificity of surveillance case definitions for Zika virus disease. Emerg. Infect. Dis. 23(4), 677\u2013679 (2017).","journal-title":"Emerg. Infect. Dis."},{"key":"42373_CR29","doi-asserted-by":"crossref","unstructured":"Mastin, A. J., Van Den Bosch, F., Gottwald, T. R., Alonso Chavez, V. & Parnell, S. R. A method of determining where to target surveillance efforts in heterogeneous epidemiological systems. Tanaka MM, editor. PLoS Comput Biol. ;13(8):e1005712. (2017).","DOI":"10.1371\/journal.pcbi.1005712"},{"key":"42373_CR30","doi-asserted-by":"crossref","unstructured":"Jaudou, S., Tran, M. L., Vorimore, F., Fach, P. & Delannoy, S. Evaluation of high molecular weight DNA extraction methods for long-read sequencing of Shiga toxin-producing Escherichia coli. Eppinger M, editor. PLoS ONE. ;17(7):e0270751. (2022).","DOI":"10.1371\/journal.pone.0270751"},{"issue":"1","key":"42373_CR31","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1146\/annurev-pathmechdis-012418-012751","volume":"14","author":"W Gu","year":"2019","unstructured":"Gu, W., Miller, S. & Chiu, C. Y. Clinical metagenomic next-generation sequencing for pathogen detection. Annu. Rev. Pathol. Mech. Dis. 14(1), 319\u2013338 (2019).","journal-title":"Annu. Rev. Pathol. Mech. Dis."},{"issue":"6","key":"42373_CR32","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1038\/s41576-019-0113-7","volume":"20","author":"CY Chiu","year":"2019","unstructured":"Chiu, C. Y. & Miller, S. A. Clinical metagenomics. Nat. Rev. Genet. 20(6), 341\u201355 (2019).","journal-title":"Nat. Rev. Genet."},{"key":"42373_CR33","doi-asserted-by":"publisher","first-page":"236","DOI":"10.3389\/fcimb.2018.00236","volume":"8","author":"A Estrada-Pe\u00f1a","year":"2018","unstructured":"Estrada-Pe\u00f1a, A., Cabezas-Cruz, A., Pollet, T., Vayssier-Taussat, M. & Cosson, J. F. High Throughput Sequencing and Network Analysis Disentangle the Microbial Communities of Ticks and Hosts Within and Between Ecosystems. Front. Cell. Infect. Microbiol. 8, 236 (2018).","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"suppl_2","key":"42373_CR34","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/jmedent\/15.suppl2.1","volume":"15","author":"JE Keirans","year":"1978","unstructured":"Keirans, J. E. & Clifford, C. M. The Genus Ixodes in the United States: A Scanning Electron Microscope Study and Key to the Adults1. J. Med. Entomol. 15 (suppl_2), 1\u201338 (1978).","journal-title":"J. Med. Entomol."},{"key":"42373_CR35","unstructured":"Cooley, R. A. & Kohls, G. M. The Genus Ixodes in North America [Internet]. U.S. Government Printing Office; (Bulletin). (1945). Available from: https:\/\/books.google.com\/books?id=uzNDAAAAYAAJ"},{"key":"42373_CR36","doi-asserted-by":"crossref","unstructured":"Wills, M. K. B., Kirby, A. M. & Lloyd, V. K. Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR. JoVE ;(132):56471. (2018).","DOI":"10.3791\/56471"},{"issue":"14","key":"42373_CR37","doi-asserted-by":"publisher","first-page":"4078","DOI":"10.1111\/mec.16985","volume":"32","author":"NM Fountain-Jones","year":"2023","unstructured":"Fountain-Jones, N. M. et al. Positive associations matter: Microbial relationships drive tick microbiome composition. Mol Ecol 32(14), 4078\u201392 (2023).","journal-title":"Mol Ecol"},{"issue":"3","key":"42373_CR38","doi-asserted-by":"publisher","first-page":"e191","DOI":"10.1002\/imt2.191","volume":"3","author":"W Shen","year":"2024","unstructured":"Shen, W., Sipos, B. & Zhao, L. SeqKit2: A Swiss army knife for sequence and alignment processing. iMeta 3 (3), e191 (2024).","journal-title":"iMeta"},{"issue":"69","key":"42373_CR39","doi-asserted-by":"publisher","first-page":"2991","DOI":"10.21105\/joss.02991","volume":"7","author":"E Steinig","year":"2022","unstructured":"Steinig, E. & Coin, L. Nanoq: Ultra-fast quality control for nanopore reads. JOSS 7(69), 2991 (2022).","journal-title":"JOSS"},{"key":"42373_CR40","doi-asserted-by":"crossref","unstructured":"De Coster, W. & Rademakers, R. NanoPack2: population-scale evaluation of long-read sequencing data. Alkan C, editor. Bioinformatics. ;39(5):btad311. (2023).","DOI":"10.1093\/bioinformatics\/btad311"},{"issue":"2","key":"42373_CR41","doi-asserted-by":"publisher","first-page":"giab008","DOI":"10.1093\/gigascience\/giab008","volume":"10","author":"P Danecek","year":"2021","unstructured":"Danecek, P. et al. Twelve years of SAMtools and BCFtools. GigaScience 10 (2), giab008 (2021).","journal-title":"GigaScience"},{"issue":"6","key":"42373_CR42","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","volume":"26","author":"AR Quinlan","year":"2010","unstructured":"Quinlan, A. R. & Hall, I. M. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics 26 (6), 841\u2013842 (2010).","journal-title":"Bioinformatics"},{"issue":"9","key":"42373_CR43","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1101\/gr.092759.109","volume":"19","author":"M Krzywinski","year":"2009","unstructured":"Krzywinski, M. et al. Circos: An information aesthetic for comparative genomics. Genome Res. 19(9), 1639\u201345 (2009).","journal-title":"Genome Res."},{"issue":"11","key":"42373_CR44","doi-asserted-by":"publisher","first-page":"1103","DOI":"10.1038\/s41592-020-00971-x","volume":"17","author":"M Kolmogorov","year":"2020","unstructured":"Kolmogorov, M. et al. metaFlye: Scalable long-read metagenome assembly using repeat graphs. Nat. Methods 17(11), 1103\u201310 (2020).","journal-title":"Nat. Methods"},{"issue":"1","key":"42373_CR45","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102","volume":"70","author":"A Prjibelski","year":"2020","unstructured":"Prjibelski, A., Antipov, D., Meleshko, D., Lapidus, A. & Korobeynikov, A. Using SPAdes de novo assembler. CP in Bioinformatics 70(1), e102 (2020).","journal-title":"CP in Bioinformatics"},{"issue":"5","key":"42373_CR46","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1101\/gr.215087.116","volume":"27","author":"S Koren","year":"2017","unstructured":"Koren, S. et al. Canu: Scalable and accurate long-read assembly via adaptive k -mer weighting and repeat separation. Genome Res. 27(5), 722\u201336 (2017).","journal-title":"Genome Res."},{"key":"42373_CR47","unstructured":"Wickham, H., Fran\u00e7ois, R., Henry, L., M\u00fcller, K. & Vaughan, D. dplyr: A Grammar of Data Manipulation [Internet]. R package version 1.1.4, (2023). https:\/\/github.com\/tidyverse\/dplyr. Available from: https:\/\/dplyr.tidyverse.org"},{"key":"42373_CR48","unstructured":"Wickham, H., Vaughan, D. & Girlich, M. tidyr: Tidy Messy Data. R package version 1.3.1 [Internet]. (2025). Available from: https:\/\/tidyr.tidyverse.org"},{"key":"42373_CR49","unstructured":"Wickham, H. ggplot2: Elegant Graphics for Data Analysis. 2nd ed. Cham: Springer International Publishing: Imprint: Springer; 2016. 1 p. (Use R!). (2016)."},{"key":"42373_CR50","unstructured":"Xiao, N. Scientific Journal and Sci-Fi Themed Color Palettes for ggplot2. R package version 3.2.0, (2024). https:\/\/github.com\/nanxstats\/ggsci, https:\/\/nanx.me\/ggsci\/"},{"key":"42373_CR51","unstructured":"Schloerke, B. et al. GGally: Extension to ggplot2. R package version 2.3.0, (2025). https:\/\/ggobi.github.io\/ggally\/"},{"key":"42373_CR52","unstructured":"Wickham, H., Pedersen, T. L. & Seidel, D. scales: Scale Functions for Visualization. R package version 1.4.0, (2025). https:\/\/scales.r-lib.org"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-42373-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-42373-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-42373-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T17:02:20Z","timestamp":1777050140000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-42373-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,10]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["42373"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-42373-7","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,10]]},"assertion":[{"value":"11 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 February 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 March 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"12914"}}