{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:01:20Z","timestamp":1780448480299,"version":"3.54.1"},"reference-count":57,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:00:00Z","timestamp":1775865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":53,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32271013"],"award-info":[{"award-number":["32271013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32070811"],"award-info":[{"award-number":["32070811"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-026-45291-w","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T15:04:17Z","timestamp":1775919857000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic morphological staging of Drosophila pupal development"],"prefix":"10.1038","volume":"16","author":[{"given":"Xu","family":"Liwen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhang","family":"Yumeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pang","family":"Xuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tang","family":"Jiaxu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhang","family":"Chenchen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geng","family":"Junhua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xie","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gan","family":"Guangming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,11]]},"reference":[{"issue":"3","key":"45291_CR1","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1046\/j.1525-142x.2000.00057.x","volume":"2","author":"C Nielsen","year":"2000","unstructured":"Nielsen, C. The origin of metamorphosis. Evol. Dev. 2(3), 127\u2013129 (2000).","journal-title":"Evol. Dev."},{"issue":"6752","key":"45291_CR2","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1038\/46737","volume":"401","author":"JW Truman","year":"1999","unstructured":"Truman, J. W. & Riddiford, L. M. The origins of insect metamorphosis. Nature 401(6752), 447\u2013452 (1999).","journal-title":"Nature"},{"issue":"1","key":"45291_CR3","first-page":"20","volume":"306","author":"DD Brown","year":"2007","unstructured":"Brown, D. D. & Cai, L. Amphibian metamorphosis Dev. Biol., 306(1): 20\u201333. (2007).","journal-title":"Amphibian metamorphosis Dev. Biol."},{"key":"45291_CR4","doi-asserted-by":"publisher","first-page":"244","DOI":"10.3389\/fphys.2020.00244","volume":"11","author":"Y Cheng","year":"2020","unstructured":"Cheng, Y. et al. Expression and Regulation of pde6h by Thyroid Hormone During Metamorphosis in Paralichthys olivaceus. Front. Physiol. 11, 244 (2020).","journal-title":"Front. Physiol."},{"issue":"4","key":"45291_CR5","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1007\/s42995-023-00204-y","volume":"5","author":"F Xu","year":"2023","unstructured":"Xu, F. et al. Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster (Crassostrea gigas). Mar. Life Sci. Technol. 5(4), 467\u2013477 (2023).","journal-title":"Mar. Life Sci. Technol."},{"issue":"23","key":"45291_CR6","doi-asserted-by":"publisher","first-page":"R1252","DOI":"10.1016\/j.cub.2019.10.009","volume":"29","author":"JW Truman","year":"2019","unstructured":"Truman, J. W. The Evolution of Insect Metamorphosis. Curr. Biol. 29(23), R1252\u2013R1268 (2019).","journal-title":"Curr. Biol."},{"key":"45291_CR7","doi-asserted-by":"crossref","unstructured":"Belles, X. Investigating the origin of insect metamorphosis. Elife 12 (2023).","DOI":"10.7554\/eLife.94410"},{"issue":"1783","key":"45291_CR8","doi-asserted-by":"publisher","first-page":"20180415","DOI":"10.1098\/rstb.2018.0415","volume":"374","author":"X Belles","year":"2019","unstructured":"Belles, X. The innovation of the final moult and the origin of insect metamorphosis. Philos. Trans. R Soc. Lond. B Biol. Sci. 374(1783), 20180415 (2019).","journal-title":"Philos. Trans. R Soc. Lond. B Biol. Sci."},{"key":"45291_CR9","unstructured":"Belles, X. Origin and Evolution of Insect Metamorphosis. in eLS."},{"issue":"1","key":"45291_CR10","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.ydbio.2009.01.032","volume":"329","author":"J Chesebro","year":"2009","unstructured":"Chesebro, J. et al. Diverging functions of Scr between embryonic and post-embryonic development in a hemimetabolous insect, Oncopeltus fasciatus. Dev. Biol. 329(1), 142\u2013151 (2009).","journal-title":"Dev. Biol."},{"key":"45291_CR11","doi-asserted-by":"crossref","unstructured":"Gijbels, M. et al. RNAi-mediated knockdown of transcription factor E93 in nymphs of the desert locust (Schistocerca gregaria) inhibits adult morphogenesis and results in supernumerary juvenile stages. Int. J. Mol. Sci., 21(20) (2020).","DOI":"10.3390\/ijms21207518"},{"issue":"51","key":"45291_CR12","doi-asserted-by":"publisher","first-page":"21678","DOI":"10.1073\/pnas.0907391106","volume":"106","author":"E Gomez-Orte","year":"2009","unstructured":"Gomez-Orte, E. & Belles, X. MicroRNA-dependent metamorphosis in hemimetabolan insects. Proc. Natl. Acad. Sci. U S A. 106(51), 21678\u201321682 (2009).","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"issue":"3","key":"45291_CR13","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1007\/s12038-010-0047-x","volume":"35","author":"TC Roat","year":"2010","unstructured":"Roat, T. C. & Landim Cda, C. Mitosis and cell death in the optic lobes of workers, queens and drones of the honey bee (Apis mellifera) during metamorphosis. J. Biosci. 35(3), 415\u2013425 (2010).","journal-title":"J. Biosci."},{"issue":"3","key":"45291_CR14","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1093\/icb\/icad009","volume":"63","author":"CC Rittschof","year":"2023","unstructured":"Rittschof, C. C. & Denny, A. S. The Impacts of Early-Life Experience on Bee Phenotypes and Fitness. Integr. Comp. Biol. 63(3), 808\u2013824 (2023).","journal-title":"Integr. Comp. Biol."},{"issue":"8","key":"45291_CR15","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1002\/jez.b.23278","volume":"342","author":"A Rajakumar","year":"2024","unstructured":"Rajakumar, A. et al. From Egg to Adult: A Developmental Table of the Ant Monomorium pharaonis. J. Exp. Zool. B Mol. Dev. Evol. 342(8), 557\u2013585 (2024).","journal-title":"J. Exp. Zool. B Mol. Dev. Evol."},{"issue":"3","key":"45291_CR16","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1038\/s41588-021-00809-y","volume":"53","author":"C K\u00fcpper","year":"2021","unstructured":"K\u00fcpper, C. Metamorphosis of a butterfly inversion. Nat. Genet. 53(3), 264\u2013266 (2021).","journal-title":"Nat. Genet."},{"issue":"1783","key":"45291_CR17","doi-asserted-by":"publisher","first-page":"20190063","DOI":"10.1098\/rstb.2019.0063","volume":"374","author":"J Rolff","year":"2019","unstructured":"Rolff, J., Johnston, P. R. & Reynolds, S. Complete metamorphosis of insects. Philos. Trans. R Soc. Lond. B Biol. Sci. 374(1783), 20190063 (2019).","journal-title":"Philos. Trans. R Soc. Lond. B Biol. Sci."},{"issue":"23","key":"45291_CR18","doi-asserted-by":"publisher","first-page":"6543","DOI":"10.1111\/mec.16673","volume":"32","author":"C Manthey","year":"2023","unstructured":"Manthey, C. et al. Complete metamorphosis and microbiota turnover in insects. Mol. Ecol. 32(23), 6543\u20136551 (2023).","journal-title":"Mol. Ecol."},{"issue":"19","key":"45291_CR19","doi-asserted-by":"publisher","first-page":"R868","DOI":"10.1016\/j.cub.2015.07.059","volume":"25","author":"MS Engel","year":"2015","unstructured":"Engel, M. S. Insect evolution. Curr. Biol. 25(19), R868\u2013R872 (2015).","journal-title":"Curr. Biol."},{"issue":"1496","key":"45291_CR20","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1098\/rstb.2007.2244","volume":"363","author":"AD Peel","year":"2008","unstructured":"Peel, A. D. The evolution of developmental gene networks: lessons from comparative studies on holometabolous insects. Philos. Trans. R Soc. Lond. B Biol. Sci. 363(1496), 1539\u20131547 (2008).","journal-title":"Philos. Trans. R Soc. Lond. B Biol. Sci."},{"issue":"3","key":"45291_CR21","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1534\/genetics.115.183392","volume":"201","author":"KG Hales","year":"2015","unstructured":"Hales, K. G. et al. Genetics on the Fly: A Primer on the Drosophila Model System. Genetics 201(3), 815\u2013842 (2015).","journal-title":"Genetics"},{"key":"45291_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cois.2020.08.008","volume":"43","author":"BJ Thompson","year":"2021","unstructured":"Thompson, B. J. From genes to shape during metamorphosis: a history. Curr. Opin. Insect Sci. 43, 1\u201310 (2021).","journal-title":"Curr. Opin. Insect Sci."},{"key":"45291_CR23","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1007\/978-1-0716-0585-1_23","volume":"2143","author":"EJ Brace","year":"2020","unstructured":"Brace, E. J. & DiAntonio, A. Models of Axon Degeneration in Drosophila Larvae. Methods Mol. Biol. 2143, 311\u2013320 (2020).","journal-title":"Methods Mol. Biol."},{"issue":"4","key":"45291_CR24","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1007\/s00412-016-0595-4","volume":"125","author":"JV Beira","year":"2016","unstructured":"Beira, J. V. & Paro, R. The legacy of Drosophila imaginal discs. Chromosoma 125(4), 573\u2013592 (2016).","journal-title":"Chromosoma"},{"issue":"1","key":"45291_CR25","doi-asserted-by":"publisher","first-page":"5164","DOI":"10.1038\/s41598-021-84639-2","volume":"11","author":"G Okude","year":"2021","unstructured":"Okude, G., Fukatsu, T. & Futahashi, R. Comprehensive comparative morphology and developmental staging of final instar larvae toward metamorphosis in the insect order Odonata. Sci. Rep. 11(1), 5164 (2021).","journal-title":"Sci. Rep."},{"key":"45291_CR26","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/1749-8104-6-15","volume":"6","author":"A Winbush","year":"2011","unstructured":"Winbush, A. & Weeks, J. C. Steroid-triggered, cell-autonomous death of a Drosophila motoneuron during metamorphosis. Neural Dev. 6, 15 (2011).","journal-title":"Neural Dev."},{"key":"45291_CR27","doi-asserted-by":"publisher","first-page":"40873","DOI":"10.1038\/srep40873","volume":"7","author":"S Barish","year":"2017","unstructured":"Barish, S. et al. Transcriptional profiling of olfactory system development identifies distal antenna as a regulator of subset of neuronal fates. Sci. Rep. 7, 40873 (2017).","journal-title":"Sci. Rep."},{"key":"45291_CR28","first-page":"57","volume":"66","author":"SP Bainbridge","year":"1981","unstructured":"Bainbridge, S. P. & Bownes, M. Staging the metamorphosis of Drosophila melanogaster. J. Embryol. Exp. Morphol. 66, 57\u201380 (1981).","journal-title":"J. Embryol. Exp. Morphol."},{"key":"45291_CR29","doi-asserted-by":"crossref","unstructured":"Schoborg, T. A. et al. Micro-computed tomography as a platform for exploring Drosophila development. Development 146(23) (2019).","DOI":"10.1242\/dev.176685"},{"issue":"1","key":"45291_CR30","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1006\/dbio.1999.9343","volume":"212","author":"G Lam","year":"1999","unstructured":"Lam, G. et al. DHR3 is required for the prepupal-pupal transition and differentiation of adult structures during Drosophila metamorphosis. Dev. Biol. 212(1), 204\u2013216 (1999).","journal-title":"Dev. Biol."},{"issue":"3","key":"45291_CR31","doi-asserted-by":"publisher","first-page":"1621","DOI":"10.1534\/genetics.105.045641","volume":"172","author":"Y Ji","year":"2006","unstructured":"Ji, Y. & Clark, D. V. The purine synthesis gene Prat2 is required for Drosophila metamorphosis, as revealed by inverted-repeat-mediated RNA interference. Genetics 172(3), 1621\u20131631 (2006).","journal-title":"Genetics"},{"issue":"32","key":"45291_CR32","doi-asserted-by":"publisher","first-page":"14217","DOI":"10.1073\/pnas.1008623107","volume":"107","author":"S Aldaz","year":"2010","unstructured":"Aldaz, S., Escudero, L. M. & Freeman, M. Live imaging of Drosophila imaginal disc development. Proc. Natl. Acad. Sci. U S A. 107(32), 14217\u201314222 (2010).","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"issue":"1","key":"45291_CR33","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.ydbio.2009.06.021","volume":"333","author":"LY Rayburn","year":"2009","unstructured":"Rayburn, L. Y. et al. The proprotein convertase amontillado (amon) is required during Drosophila pupal development. Dev. Biol. 333(1), 48\u201356 (2009).","journal-title":"Dev. Biol."},{"key":"45291_CR34","unstructured":"Fukutomi, Y. et al. Methods for staging pupal periods and measurement of wing pigmentation of Drosophila guttifera. J. Vis. Exp. 131 (2013)."},{"issue":"3","key":"45291_CR35","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1007\/s00427-017-0578-3","volume":"227","author":"Y Fukutomi","year":"2017","unstructured":"Fukutomi, Y. et al. Pupal development and pigmentation process of a polka-dotted fruit fly, Drosophila guttifera (Insecta, Diptera). Dev. Genes Evol. 227(3), 171\u2013180 (2017).","journal-title":"Dev. Genes Evol."},{"issue":"1","key":"45291_CR36","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1101\/gad.229518.113","volume":"28","author":"OE Tasdemir-Yilmaz","year":"2014","unstructured":"Tasdemir-Yilmaz, O. E. & Freeman, M. R. Astrocytes engage unique molecular programs to engulf pruned neuronal debris from distinct subsets of neurons. Genes Dev. 28(1), 20\u201333 (2014).","journal-title":"Genes Dev."},{"key":"45291_CR37","doi-asserted-by":"crossref","unstructured":"J\u00fcrgens, K. J., Drechsler, M. & Paululat, A. An anatomical atlas of Drosophila melanogaster-the wild-type. Genetics 228(2) (2024).","DOI":"10.1093\/genetics\/iyae129"},{"key":"45291_CR38","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.neures.2016.09.005","volume":"116","author":"X Cao","year":"2017","unstructured":"Cao, X. & Tabuchi, K. Functions of synapse adhesion molecules neurexin\/neuroligins and neurodevelopmental disorders. Neurosci. Res. 116, 3\u20139 (2017).","journal-title":"Neurosci. Res."},{"key":"45291_CR39","doi-asserted-by":"publisher","first-page":"1257347","DOI":"10.3389\/fncel.2023.1257347","volume":"17","author":"G Guangming","year":"2023","unstructured":"Guangming, G. et al. Neurexin and neuroligins jointly regulate synaptic degeneration at the Drosophila neuromuscular junction based on TEM studies. Front. Cell. Neurosci. 17, 1257347 (2023).","journal-title":"Front. Cell. Neurosci."},{"issue":"5","key":"45291_CR40","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1016\/j.neuron.2007.08.002","volume":"55","author":"J Li","year":"2007","unstructured":"Li, J. et al. Crucial role of Drosophila neurexin in proper active zone apposition to postsynaptic densities, synaptic growth, and synaptic transmission. Neuron 55(5), 741\u2013755 (2007).","journal-title":"Neuron"},{"key":"45291_CR41","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3389\/fnana.2020.00019","volume":"14","author":"G Guangming","year":"2020","unstructured":"Guangming, G. et al. Neurexin and Neuroligins Maintain the Balance of Ghost and Satellite Boutons at the Drosophila Neuromuscular Junction. Front. Neuroanat. 14, 19 (2020).","journal-title":"Front. Neuroanat."},{"issue":"2","key":"45291_CR42","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1523\/JNEUROSCI.3854-10.2011","volume":"31","author":"M Sun","year":"2011","unstructured":"Sun, M. et al. Neuroligin 2 is required for synapse development and function at the Drosophila neuromuscular junction. J. Neurosci. 31(2), 687\u2013699 (2011).","journal-title":"J. Neurosci."},{"issue":"46","key":"45291_CR43","doi-asserted-by":"publisher","first-page":"31867","DOI":"10.1074\/jbc.M114.574897","volume":"289","author":"G Xing","year":"2014","unstructured":"Xing, G. et al. Drosophila neuroligin3 regulates neuromuscular junction development and synaptic differentiation. J. Biol. Chem. 289(46), 31867\u201331877 (2014).","journal-title":"J. Biol. Chem."},{"issue":"44","key":"45291_CR44","doi-asserted-by":"publisher","first-page":"17991","DOI":"10.1074\/jbc.M117.810242","volume":"292","author":"X Zhang","year":"2017","unstructured":"Zhang, X. et al. Neuroligin 4 regulates synaptic growth via the bone morphogenetic protein (BMP) signaling pathway at the Drosophila neuromuscular junction. J. Biol. Chem. 292(44), 17991\u201318005 (2017).","journal-title":"J. Biol. Chem."},{"issue":"5638","key":"45291_CR45","first-page":"535","volume":"41","author":"M Ashburner","year":"1989","unstructured":"Ashburner, M., Golic, K. & Hawley, R. J. D. Drosophila: Lab. Handb. 41(5638): 535\u2013536. (1989).","journal-title":"Drosophila: Lab. Handb."},{"issue":"6775","key":"45291_CR46","doi-asserted-by":"publisher","first-page":"eady5541","DOI":"10.1126\/science.ady5541","volume":"390","author":"BJ Choi","year":"2025","unstructured":"Choi, B. J., Chen, Y. C. & Desplan, C. Retinal calcium waves coordinate uniform tissue patterning of the Drosophila eye. Science 390(6775), eady5541 (2025).","journal-title":"Science"},{"issue":"11","key":"45291_CR47","doi-asserted-by":"publisher","first-page":"1436","DOI":"10.1002\/jmor.21257","volume":"281","author":"A Campoy","year":"2020","unstructured":"Campoy, A., P\u00e9rez-Ba\u00f1\u00f3n, C. & Rojo, S. Intra-puparial development in the hoverflies Eristalinus aeneus and Eristalis tenax (Diptera: Syrphidae). J. Morphol. 281(11), 1436\u20131445 (2020).","journal-title":"J. Morphol."},{"key":"45291_CR48","doi-asserted-by":"crossref","unstructured":"Xu, F. et al. Mounting of Embryos, Larvae, and Pupae for Live Drosophila Dendritic Arborization Neuron Imaging (Cold Spring Harb Protoc, 2023).","DOI":"10.1101\/pdb.prot108149"},{"key":"45291_CR49","doi-asserted-by":"crossref","unstructured":"Tann, J. Y. et al. Culture of Larval and Pupal Drosophila Dendritic Arborization Neurons (Cold Spring Harb Protoc, 2023).","DOI":"10.1101\/pdb.prot108150"},{"issue":"6","key":"45291_CR50","doi-asserted-by":"publisher","first-page":"652","DOI":"10.1101\/pdb.prot5629","volume":"2011","author":"J Morante","year":"2011","unstructured":"Morante, J. & Desplan, C. Dissection and staining of Drosophila optic lobes at different stages of development. Cold Spring Harb Protoc. 2011(6), 652\u2013656 (2011).","journal-title":"Cold Spring Harb Protoc."},{"issue":"6","key":"45291_CR51","doi-asserted-by":"publisher","first-page":"e38714","DOI":"10.1371\/journal.pone.0038714","volume":"7","author":"A Kawamori","year":"2012","unstructured":"Kawamori, A., Shimaji, K. & Yamaguchi, M. Dynamics of endoreplication during Drosophila posterior scutellar macrochaete development. PLoS One. 7(6), e38714 (2012).","journal-title":"PLoS One"},{"issue":"2","key":"45291_CR52","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1242\/dev.107.2.389","volume":"107","author":"V Hartenstein","year":"1989","unstructured":"Hartenstein, V. & Posakony, J. W. Development of adult sensilla on the wing and notum of Drosophila melanogaster. Development 107(2), 389\u2013405 (1989).","journal-title":"Development"},{"key":"45291_CR53","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1186\/s12861-014-0046-5","volume":"14","author":"K Taniguchi","year":"2014","unstructured":"Taniguchi, K. et al. Isoform-specific functions of Mud\/NuMA mediate binucleation of Drosophila male accessory gland cells. BMC Dev. Biol. 14, 46 (2014).","journal-title":"BMC Dev. Biol."},{"issue":"6","key":"45291_CR54","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1016\/S0092-8674(00)81800-0","volume":"87","author":"S Baumgartner","year":"1996","unstructured":"Baumgartner, S. et al. A Drosophila neurexin is required for septate junction and blood-nerve barrier formation and function. Cell 87(6), 1059\u20131068 (1996).","journal-title":"Cell"},{"issue":"13","key":"45291_CR55","doi-asserted-by":"publisher","first-page":"2509","DOI":"10.1016\/j.febslet.2007.04.068","volume":"581","author":"X Zeng","year":"2007","unstructured":"Zeng, X. et al. Neurexin-1 is required for synapse formation and larvae associative learning in Drosophila. FEBS Lett. 581(13), 2509\u20132516 (2007).","journal-title":"FEBS Lett."},{"issue":"1","key":"45291_CR56","doi-asserted-by":"publisher","first-page":"1056","DOI":"10.1038\/s42003-023-05428-3","volume":"6","author":"Y Sun","year":"2023","unstructured":"Sun, Y. et al. Neuroligin 2 governs synaptic morphology and function through RACK1-cofilin signaling in Drosophila. Commun. Biol. 6(1), 1056 (2023).","journal-title":"Commun. Biol."},{"issue":"39","key":"45291_CR57","doi-asserted-by":"publisher","first-page":"15545","DOI":"10.1523\/JNEUROSCI.0819-13.2013","volume":"33","author":"Y Li","year":"2013","unstructured":"Li, Y. et al. Drosophila neuroligin 4 regulates sleep through modulating GABA transmission. J. Neurosci. 33(39), 15545\u201315554 (2013).","journal-title":"J. Neurosci."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-45291-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-45291-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-45291-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:04:19Z","timestamp":1780445059000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-45291-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,11]]},"references-count":57,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["45291"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-45291-w","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,11]]},"assertion":[{"value":"11 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 March 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 April 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"17037"}}