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Increasing resistance of\n                    <jats:italic>Pseudomonas syringae<\/jats:italic>\n                    to conventional management strategies highlights the need for alternative disease control options. In this study, we isolated and characterized three lytic bacteriophages, including vB_PsyP_Mobley, vB_PsyP_Plaza, and vB_PsyP_Mission, targeting almond-infecting\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    strains. Host-range analysis across 36 isolates revealed partially overlapping infectivity profiles, with strongest activity against phylogroup 2 (PG2) almond-associated\n                    <jats:italic>P. syringae<\/jats:italic>\n                    pv.\n                    <jats:italic>syringae<\/jats:italic>\n                    and reduced infectivity against phylogenetically distinct isolates, including PG7\n                    <jats:italic>P. viridiflava<\/jats:italic>\n                    and other crop-associated pathovars. Efficiency-of-plating assays quantitatively supported these host-range patterns. Plaque morphology and transmission electron microscopy demonstrated icosahedral capsids with short, non-contractile tails consistent with tailed dsDNA bacteriophages of the class\n                    <jats:italic>Caudoviricetes<\/jats:italic>\n                    , with vB_PsyP_Mission producing halo-associated plaques consistent with predicted extracellular polysaccharide-modifying proteins. Genomic analyses revealed compact (~\u200940\u00a0kb) genomes lacking integrases, tRNAs, lysogeny-associated genes, and known virulence or antimicrobial resistance determinants. Comparative genomics showed that vB_PsyP_Plaza and vB_PsyP_Mission likely represent novel isolates within previously described species-level groups, whereas vB_PsyP_Mobley is more genomically divergent. Collectively, these results define the phenotypic and genomic characteristics of three related but distinguishable\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    phages and provide a basis for future\n                    <jats:italic>in planta<\/jats:italic>\n                    evaluation of their potential utility against almond bacterial blast.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-47496-5","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T07:07:18Z","timestamp":1775545638000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and biological characterization of lytic phages infecting Pseudomonas syringae associated with almond bacterial blast"],"prefix":"10.1038","volume":"16","author":[{"given":"Cuong V.","family":"Hoang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren","family":"Bhasin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley","family":"Duran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Quaal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suraj","family":"Ganiger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luna","family":"Santiago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James E.","family":"Adaskaveg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tawanda E.","family":"Maguvu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florent P.","family":"Trouillas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olakunle I.","family":"Olawole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,4,7]]},"reference":[{"key":"47496_CR1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1485197","volume":"15","author":"H Alattas","year":"2024","unstructured":"Alattas, H., Glick, B. 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