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Given the emerging roles of the gut\u2013muscle axis and AMP-activated protein kinase (AMPK) in muscle homeostasis, we sought to identify gut-derived microbial strains that enhance muscle function via AMPK activation. We identified\n                    <jats:italic>Bifidobacterium animalis subsp. lactis<\/jats:italic>\n                    DS109-B11 as a potent AMPK activator. DS109-B11 microbial culture supernatant (MCS) increased AMPK phosphorylation during C2C12 myoblast differentiation, enhanced myogenic differentiation, and mitigated dexamethasone-induced myotube atrophy in vitro. In aged mice, oral administration of live DS109-B11 improved grip strength and motor performance and increased myofiber cross-sectional area, accompanied by elevated AMPK phosphorylation, upregulated mitochondrial and oxidative phosphorylation genes, and downregulated atrophy- and inflammation-related genes in skeletal muscle. In a botulinum toxin\u2013induced neurogenic atrophy model, DS109-B11 treatment partially preserved tibialis anterior muscle mass, improved myofiber cross-sectional area, and suppressed atrophy-related gene expression. These findings identify DS109-B11 as an AMPK-activating probiotic strain that beneficially modulates skeletal muscle differentiation, enhances resilience to catabolic stress, and improves muscle function in vivo.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-48725-7","type":"journal-article","created":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T13:33:52Z","timestamp":1776000832000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Probiotic Bifidobacterium animalis subsp. lactis DS109-B11 ameliorates age-related muscle weakness via AMPK activation"],"prefix":"10.1038","volume":"16","author":[{"given":"Jae Won","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Ju","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heeyeon","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seongwan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doo-Sang","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Ryoul","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Pyo","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,12]]},"reference":[{"key":"48725_CR1","doi-asserted-by":"publisher","first-page":"2636","DOI":"10.1016\/S0140-6736(19)31138-9","volume":"393","author":"AJ Cruz-Jentoft","year":"2019","unstructured":"Cruz-Jentoft, A. 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