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This study evaluated berberine\u2019s effects on the PI3K\/Akt pathway and efferocytosis in an adenocarcinoma model to reduce cisplatin chemotherapy side effects and Ehrlich ascites carcinoma (EAC) proliferation. Eighty Swiss albino mice were divided into eight groups: control, berberine, cisplatin, cisplatin &amp; berberine, EAC, EAC &amp; berberine, EAC &amp; cisplatin, and EAC &amp; cisplatin &amp; berberine. Tumor response, weight change, cell count, survival analysis, biochemical analysis, molecular studies, and histopathological assessment were performed. Results showed berberine enhanced cisplatin\u2019s antitumor efficacy in EAC-bearing mice by reducing tumor volume and cell counts. Berberine ameliorated cisplatin\u2019s hepato-renal toxicity and downregulated Akt1, Axl, Mertk, and Gas6 gene expression. Histopathological analysis showed berberine\u2019s ability to recover cisplatin\u2019s lethal effects on liver tissue. In conclusion, berberine showed promising effects on the PI3K\/Akt pathway and efferocytosis, reducing cisplatin\u2019s side effects and EAC proliferation.<\/jats:p>","DOI":"10.1038\/s41598-026-49296-3","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T10:02:00Z","timestamp":1777370520000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Berberine enhances cisplatin efficacy in ehrlich ascites carcinoma via modulation of apoptotic pathway and efferocytosis"],"prefix":"10.1038","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9108-6932","authenticated-orcid":false,"given":"Maha M.","family":"Salem","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Safia M.","family":"Dawod","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarek M.","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wafaa M.","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amira T.","family":"Khattab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"49296_CR1","doi-asserted-by":"crossref","unstructured":"Hamdy, N. 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