{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:52:18Z","timestamp":1782125538438,"version":"3.54.5"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":32,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000980","name":"Grains Research and Development Corporation","doi-asserted-by":"publisher","award":["DEE2305-004RTX"],"award-info":[{"award-number":["DEE2305-004RTX"]}],"id":[{"id":"10.13039\/501100000980","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The Australian Plague Locust (APL),\n                    <jats:italic>Chortoicetes terminifera<\/jats:italic>\n                    , the most important pest locust in Australia, can cause significant damage to crops and grasslands when present in high numbers. We have developed a new LAMP (loop-mediated isothermal amplification) assay specifically targeting APL for rapid diagnostics to support locust management. We tested five DNA extraction methods for potential near-field use and designed an APL gBlock as a synthetic positive control. The performance of new LAMP assay was assessed against a panel of eleven closely related Australian grasshopper species (family Acrididae). The optimised LAMP assay produced amplification only from APL DNA, on average in 18.0\u2009\u00b1\u20092.9\u00a0min, with an anneal derivative of 79.9\u2009\u00b1\u20090.5\u00a0\u00b0C, and gBlock produced a different anneal derivative of 83.5\u2009\u00b1\u20090.5\u00a0\u00b0C. The LAMP assay was sensitive to very low levels of DNA, with detection down to 0.2 picogram of APL DNA. The speed of application and accurate identification results generated, enables this scalable assay to be easily used in the field as a new tool for APL control, providing positive\/negative results within an hour. This capability will prove an essential aid during sporadic APL population growth events, when accurate species-level identification of substantial numbers of nymphs, prior to adult locusts swarming, is a critical aspect of pest management.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-50241-7","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T09:42:53Z","timestamp":1781257373000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Loop-mediated isothermal amplification (LAMP) assay for identification of Australian Plague Locust (APL), Chortoicetes terminifera (Walker, 1870)"],"prefix":"10.1038","volume":"16","author":[{"given":"Lea","family":"Rako","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Martoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cait","family":"Selleck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Kearney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan C.","family":"Rodoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Blacket","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,21]]},"reference":[{"key":"50241_CR1","unstructured":"APLC. Department of Agriculture, Fisheries and Forestry; Australian Plague Locust Commission. https:\/\/www.agriculture.gov.au\/biosecurity-trade\/pests-diseases-weeds\/locusts (2024)."},{"key":"50241_CR2","unstructured":"DAFF. Australian plague locust; Department of Agriculture, Fisheries and Forestry. https:\/\/www.agriculture.gov.au\/biosecurity-trade\/pests-diseases-weeds\/locusts\/about\/australia (2022)."},{"key":"50241_CR3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2025.108402","volume":"213","author":"SR Law","year":"2025","unstructured":"Law, S. R. et al. Molecular profiling of the Australian plague locust pathobiome reveals a microbial driver of population suppression. J. Invertebr. Pathol. 213, 108402 (2025).","journal-title":"J. Invertebr. Pathol."},{"key":"50241_CR4","unstructured":"Agriculture Victoria. Australian plague locusts, Agriculture Victoria. https:\/\/agriculture.vic.gov.au\/biosecurity\/pest-insects-and-mites\/priority-pest-insects-and-mites\/plague-locusts (2025)."},{"key":"50241_CR5","unstructured":"Rentz, D. C. F., Lewis, R. C., Su, Y. N. & Upton, M. S. A Guide to Australian Grasshoppers and Locusts (Natural History Publications (Borneo) Sdn. Bhd, 2003)."},{"key":"50241_CR6","unstructured":"Agriculture Victoria. Australian Plague Locust Field Surveillance Guide. https:\/\/agriculture.vic.gov.au\/biosecurity\/pest-insects-and-mites\/priority-pest-insects-and-mites\/plague-locusts\/australian-plague-locust-biology-and-behaviour (2025)."},{"key":"50241_CR7","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1665\/1082-6467(2007)16[115:AMPAOT]2.0.CO;2","volume":"16","author":"M Fries","year":"2007","unstructured":"Fries, M., Chapco, W. & Contreras, D. A molecular phylogenetic analysis of the Oedipodinae and their intercontinental relationships. J. Orthoptera Res. 16, 115\u2013125 (2007).","journal-title":"J. Orthoptera Res."},{"key":"50241_CR8","doi-asserted-by":"crossref","unstructured":"Song, H., Moulton, M. J. & Whiting, M. F. Rampant nuclear insertion of mtDNA across diverse lineages within Orthoptera (Insecta). PLoS ONE 9(10), e110508 (2014).","DOI":"10.1371\/journal.pone.0110508"},{"key":"50241_CR9","doi-asserted-by":"crossref","unstructured":"Hebert, P., Cywinska, A., Ball, S. L. & deWaard, J. Biological identification through DNA barcodes. Proc. R. Soc. Lond. B. Biol. Sci. 270, 313\u2013321 (2003).","DOI":"10.1098\/rspb.2002.2218"},{"key":"50241_CR10","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"T Notomi","year":"2000","unstructured":"Notomi, T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, e63 (2000).","journal-title":"Nucleic Acids Res."},{"key":"50241_CR11","doi-asserted-by":"publisher","first-page":"9554","DOI":"10.1038\/s41598-020-65715-5","volume":"10","author":"MJ Blacket","year":"2020","unstructured":"Blacket, M. J. et al. A LAMP assay for detection of Bactrocera tryoni Queensland fruit fly (Diptera: Tephiritidae). Sci. Rep. 10, 9554 (2020).","journal-title":"Sci. Rep."},{"key":"50241_CR12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-020-77928-9","volume":"10","author":"A Agarwal","year":"2020","unstructured":"Agarwal, A., Cunningham, J. P., Valenzuela, I. & Blacket, M. J. A diagnostic LAMP assay for the destructive grapevine insect pest, phylloxera (Daktulosphaira vitifoliae). Sci. Rep. 10, 1\u201310 (2020).","journal-title":"Sci. Rep."},{"issue":"12","key":"50241_CR13","doi-asserted-by":"publisher","first-page":"5509","DOI":"10.1002\/ps.6591","volume":"77","author":"L Rako","year":"2021","unstructured":"Rako, L. et al. A LAMP (Loop-mediated isothermal amplification) test for rapid identification of Khapra beetle (Trogoderma granarium). Pest Manag. Sci. 77(12), 5509\u20135521 (2021).","journal-title":"Pest Manag. Sci."},{"issue":"1","key":"50241_CR14","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-04496-x","volume":"12","author":"A Agarwal","year":"2022","unstructured":"Agarwal, A. et al. A diagnostic LAMP assay for rapid identification of an invasive plant pest, fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). Sci. Rep. 12(1), 1116 (2022).","journal-title":"Sci. Rep."},{"key":"50241_CR15","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-38860-w","volume":"13","author":"L Rako","year":"2023","unstructured":"Rako, L. et al. LAMP (Loop-mediated isothermal amplification) assay for rapid identification of Varroa mites. Sci. Rep. 13, 11931 (2023).","journal-title":"Sci. Rep."},{"key":"50241_CR16","unstructured":"Key, K. H. L. The Taxonomy, Phases, and Distribution of the Genera Chortoicetes Brunn. and Austroicetes Uv. (Orthoptera: Acrididae) (Division of Entomology, Commonwealth Scientific and Industrial Research Organization, 1954)."},{"key":"50241_CR17","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1093\/mollus\/eyy011","volume":"84","author":"A Zieritz","year":"2018","unstructured":"Zieritz, A. et al. Development and evaluation of HotShot protocols for cost- and time-effective extraction of PCR-ready DNA from single freshwater mussel larvae (Bivalvia: Unionida). J. Molluscan Stud. 84, 198\u2013201 (2018).","journal-title":"J. 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MEGA11: Molecular evolutionary genetics analysis version 11. Mol. Biol. Evol. 38, 3022\u20133027 (2021).","journal-title":"Mol. Biol. Evol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-50241-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-50241-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-50241-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:26:00Z","timestamp":1782123960000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-50241-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,21]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["50241"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-50241-7","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,21]]},"assertion":[{"value":"15 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 April 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 May 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"19352"}}