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In the current study, eight Egyptian duckling farms from different breeds (Moulard, Muscovy, and Pekin) with a history of sudden death accompanied by neurological signs with marked hepatitis lesions at Al-Qalyubia, Al-Sharqia, and Assuit governorates from 2022 to 2023 have been investigated. All the examined freshly dead ducklings have a history of vaccination with the live attenuated duck hepatitis vaccine. During the laboratory investigations, DAstV was successfully isolated on the chorioallantoic membrane (CAM) of specific pathogen-free (SPF) embryonated chicken eggs (ECE) and designated as DAstV- VSVRI-CLEVB-2022 and showed the characteristic star-shaped six-point end of astrovirus when examined under the electron microscope. Molecular identification showed positive PCR amplification targeting the 434\u00a0bp of the ORF1b encoding non-structural proteins and is highly conserved of the G4260 Avastrovirus 2, sequence analysis showed that the isolated strain showed 100% similarity with duck astrovirus strain HBGT-China-2014 (KX290465.1). Pathogenicity testing of the isolated astrovirus in susceptible one-day-old ducklings, showed typical death signs of astrovirus at 31, 38, and 50\u00a0h post-infection (PI) with paint brush hemorrhagic liver surface. The above-mentioned results showed that DAstV could be one of the causative agents of acute duck hepatitis in Egypt and further investigations are recommended to prepare a local autogenous vaccine from the isolated strain with the application of strict biosecurity measures and evaluate its efficacy against the field challenge to control such a problem.<\/jats:p>","DOI":"10.1038\/s41598-026-50596-x","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T10:55:41Z","timestamp":1779447341000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pathomolecular characterization of recently isolated duck Astrovirus from domestic ducklings in Egypt"],"prefix":"10.1038","volume":"16","author":[{"given":"Eman M.S.","family":"EL-Nagar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha A.N.","family":"Gamal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"El-Saied","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed R.","family":"Mousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Selim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba M.","family":"Salem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"50596_CR1","doi-asserted-by":"crossref","unstructured":"Zhao, Y. et al. 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