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This study was performed to characterize the bioactive compounds of the 80% methanol extract of\n                    <jats:italic>S. balansae<\/jats:italic>\n                    leaves using HPLC-ESI\/MS-MS technique, coupled with the assessment of\n                    <jats:italic>in vitro<\/jats:italic>\n                    anti-aging enzyme inhibition assays. Further, total phenolic and flavonoid content and molecular docking studies were conducted. High performance liquid chromatography-electrospray ionization coupled with mass fragmentation (HPLC-ESI\/MS-MS) was used to tentatively identify the phyto-constituents of\n                    <jats:italic>S. balansae<\/jats:italic>\n                    leaf extract. The phenolic and flavonoid contents were assessed as gallic acid equivalents (GAE) and rutin equivalents (RE). Further, the anti-skin aging potential was investigated\n                    <jats:italic>via<\/jats:italic>\n                    assessment of elastase and collagenase inhibition assays. The in silico molecular docking studies were conducted on 7EST elastase and 2TCl collagenase. The HPLC\/MS analysis revealed the identification of twenty-two compounds of different chemical classes like flavonoids, phenolic acids, and tannins. The total phenolic and flavonoid contents were 399.29\u2009\u00b1\u200913.23\u00a0\u00b5g gallic acid equivalent\/mg and 8.25\u2009\u00b1\u20090.63\u00a0\u00b5g rutin equivalent\/mg, respectively. The extract exhibited significant collagenase (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200948.08\u2009\u00b1\u20093.42\u00a0\u00b5g\/mL) and elastase (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 18.02\u2009\u00b1\u20091.57\u00a0\u00b5g\/mL) inhibitory activities, suggesting its potential in combating skin aging.\n                    <jats:italic>In silico<\/jats:italic>\n                    molecular docking studies validated the interactions of major compounds like quercetin-\n                    <jats:italic>O<\/jats:italic>\n                    -benzoyl-hexoside and 5-hydroxy-7,4'-dimethoxy-6,8-dimethyl homo-isoflavone with these enzymes. This study provides a scientific rationale for the anti-skin-aging potential of\n                    <jats:italic>S. Balansae<\/jats:italic>\n                    leaf extract, supporting its utilization in innovative anti-wrinkle formulations.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-50709-6","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:03:52Z","timestamp":1778861032000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UPLC-ESI\/MS-MS-based phytochemical analysis of Schinopsis balansae leaf extract and anti-skin ageing properties supported with in silico molecular docking experiments"],"prefix":"10.1038","volume":"16","author":[{"given":"Heba A. 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