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In the present study, the brackish water actinomycete\n                    <jats:italic>Streptomyces microflavus<\/jats:italic>\n                    BA2 was isolated from sediments of Lake Burullus (Egypt). Morphological and molecular analyses, including 16\u00a0S rRNA sequencing, confirmed its membership in the family Streptomycetaceae with 100% agreement to\n                    <jats:italic>S. microflavus<\/jats:italic>\n                    NRRL B-2156. The culture filtrate showed potent antibacterial activity against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Proteus mirabilis<\/jats:italic>\n                    ,\n                    <jats:italic>Salmonella typhi<\/jats:italic>\n                    and\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , with the aqueous diethyl ether fraction exhibiting the highest inhibition. GC-MS analyses identified seven bioactive compounds, including five fatty acids with antibacterial and anticancer properties, as well as phenolic derivatives. The diethyl ether extract showed moderate antitumor activity against HepG-2 liver cancer cells (IC\u2085\u2080 = 189.66\u00a0\u00b5g\/ml), moderate antioxidant potential (IC\u2085\u2080 = 74.15\u2009\u00b1\u20093.27\u00a0mg\/ml), and safe to moderate toxicity to normal WI-38 lung fibroblasts. These results underscore the high biomedical potential of\n                    <jats:italic>S. microflavus<\/jats:italic>\n                    BA2 as a promising source of natural agents for combating cancer and antibiotic resistance.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-51609-5","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T09:25:20Z","timestamp":1779269120000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antibacterial and anticancer properties of Streptomyces microflavus BA2 isolated from brackish waters"],"prefix":"10.1038","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9435-5510","authenticated-orcid":false,"given":"Basma M.","family":"Atallah","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6817-0151","authenticated-orcid":false,"given":"Ramadan","family":"El-domany","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2739-9012","authenticated-orcid":false,"given":"Hamdy E.","family":"Agwa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0060-3421","authenticated-orcid":false,"given":"Eithar","family":"El-Mohsnawy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"51609_CR1","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","volume":"399","author":"CJL Murray","year":"2022","unstructured":"Murray, C. 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