{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T11:35:24Z","timestamp":1786448124277,"version":"build-2736575974"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":73,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2022\/05087-6"],"award-info":[{"award-number":["2022\/05087-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-026-52613-5","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T11:15:50Z","timestamp":1780139750000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of loop-mediated isothermal amplification (LAMP)-based assay for rapid detection of fosfomycin resistance gene fosA"],"prefix":"10.1038","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3294-3815","authenticated-orcid":false,"given":"Nazareno","family":"Scaccia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inneke Marie van der Heijden","family":"Nat\u00e1rio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia Silva","family":"Figueiredo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1087-752X","authenticated-orcid":false,"given":"Silvia Figueiredo","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,30]]},"reference":[{"issue":"11","key":"52613_CR1","doi-asserted-by":"publisher","first-page":"1615","DOI":"10.3390\/ph16111615","volume":"16","author":"G Muteeb","year":"2023","unstructured":"Muteeb, G., Rehman, M. T., Shahwan, M. & Aatif, M. Origin of Antibiotics and Antibiotic Resistance, and Their Impacts on Drug Development. Narrative Rev. Pharmaceuticals. 16 (11), 1615. https:\/\/doi.org\/10.3390\/ph16111615 (2023).","journal-title":"Narrative Rev. Pharmaceuticals"},{"key":"52613_CR2","unstructured":"World Health Organization (WHO). Antimicrobial resistance. (2023). Available from: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/antimicrobial-resistance. [date visited: 08\/12\/25]."},{"key":"52613_CR3","doi-asserted-by":"publisher","first-page":"1178547","DOI":"10.3389\/fcimb.2023.1178547","volume":"13","author":"V Mattioni Marchetti","year":"2023","unstructured":"Mattioni Marchetti, V., Hrabak, J. & Bitar, I. Fosfomycin resistance mechanisms in Enterobacterales: an increasing threat. Front. Cell. Infect. Microbiol. 13, 1178547. https:\/\/doi.org\/10.3389\/fcimb.2023.1178547 (2023).","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"6","key":"52613_CR4","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1097\/QCO.0000000000000597","volume":"32","author":"M Bassetti","year":"2019","unstructured":"Bassetti, M., Graziano, E., Berruti, M. & Giacobbe, D. R. The role of fosfomycin for multidrug-resistant gram-negative infections. Curr. Opin. Infect. Dis. 32 (6), 617\u2013625. https:\/\/doi.org\/10.1097\/QCO.0000000000000597 (2019).","journal-title":"Curr. Opin. Infect. Dis."},{"key":"52613_CR5","doi-asserted-by":"publisher","unstructured":"Sorlozano-Puerto, A., Lopez-Machado, I., Albertuz-Crespo, M., Martinez-Gonzalez, L. J. & Gutierrez-Fernandez, J. Characterization of fosfomycin and nitrofurantoin resistance mechanisms in Escherichia coli isolated in clinical urine samples. Antibiotics, 1;9(9):1\u201319. (2020). https:\/\/doi.org\/10.3390\/antibiotics9090534","DOI":"10.3390\/antibiotics9090534"},{"issue":"4","key":"52613_CR6","doi-asserted-by":"publisher","first-page":"e00749","DOI":"10.1128\/mbio.00749-17","volume":"8","author":"R Ito","year":"2017","unstructured":"Ito, R. et al. Widespread Fosfomycin Resistance in Gram-Negative Bacteria Attributable to the Chromosomal fosA Gene. mBio 8 (4), e00749\u2013e00717. https:\/\/doi.org\/10.1128\/mbio.00749-17 (2017).","journal-title":"mBio"},{"issue":"12","key":"52613_CR7","doi-asserted-by":"publisher","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"T Notomi","year":"2000","unstructured":"Notomi, T. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28 (12), E63. https:\/\/doi.org\/10.1093\/nar\/28.12.e63 (2000).","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"52613_CR8","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1080\/14737159.2021.1873769","volume":"21","author":"TJ Moehling","year":"2021","unstructured":"Moehling, T. J., Choi, G., Dugan, L. C., Salit, M. & Meagher, R. J. LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community. Expert Rev. Mol. Diagn. 21 (1), 43\u201361. https:\/\/doi.org\/10.1080\/14737159.2021.1873769 (2021).","journal-title":"Expert Rev. Mol. Diagn."},{"key":"52613_CR9","doi-asserted-by":"publisher","first-page":"626","DOI":"10.1111\/jam.13647","volume":"124","author":"YP Wong","year":"2018","unstructured":"Wong, Y. P., Othman, S., Lau, Y. L., Radu, S. & Chee, H. Y. Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms. J. Appl. Microbiol. 124, 626\u2013643. https:\/\/doi.org\/10.1111\/jam.13647 (2018).","journal-title":"J. Appl. Microbiol."},{"issue":"5","key":"52613_CR10","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1038\/nprot.2008.57","volume":"3","author":"N Tomita","year":"2008","unstructured":"Tomita, N., Mori, Y., Kanda, H. & Notomi, T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat. Protoc. 3 (5), 877\u2013882. https:\/\/doi.org\/10.1038\/nprot.2008.57 (2008).","journal-title":"Nat. Protoc."},{"key":"52613_CR11","doi-asserted-by":"publisher","first-page":"100960","DOI":"10.1016\/j.nmni.2022.100960","volume":"45","author":"AD Khosravi","year":"2022","unstructured":"Khosravi, A. D. et al. The application of the loop-mediated isothermal amplification method for rapid detection of methicillin-resistant Staphylococcus aureus. New. Microbes New. Infect. 45, 100960. https:\/\/doi.org\/10.1016\/j.nmni.2022.100960 (2022).","journal-title":"New. Microbes New. Infect."},{"issue":"10","key":"52613_CR12","doi-asserted-by":"publisher","first-page":"1431","DOI":"10.1099\/jmm.0.001050","volume":"68","author":"S Lahiri","year":"2019","unstructured":"Lahiri, S. et al. Evaluation of LAMP-based assays for carbapenemase genes. J. Med. Microbiol. 68 (10), 1431\u20131437. https:\/\/doi.org\/10.1099\/jmm.0.001050 (2019).","journal-title":"J. Med. Microbiol."},{"issue":"12","key":"52613_CR13","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1111\/1348-0421.12205","volume":"58","author":"K Thirapanmethee","year":"2014","unstructured":"Thirapanmethee, K., Pothisamutyothin, K., Nathisuwan, S., Chomnawang, M. T. & Wiwat, C. Loop-mediated isothermal amplification assay targeting the blaCTX-M9 gene for detection of extended spectrum \u03b2-lactamase-producing Escherichia coli and Klebsiella pneumoniae. Microbiol. Immunol. 58 (12), 655\u2013665. https:\/\/doi.org\/10.1111\/1348-0421.12205 (2014).","journal-title":"Microbiol. Immunol."},{"issue":"10","key":"52613_CR14","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.3390\/diagnostics15101213","volume":"15","author":"EAM Mussa","year":"2025","unstructured":"Mussa, E. A. M. et al. Development of an RT-LAMP Assay for Detecting tet(M) in Enterococcus Species: Enhancing AMR Surveillance Within the One Health Sectors. Diagnostics 15 (10), 1213. https:\/\/doi.org\/10.3390\/diagnostics15101213 (2025).","journal-title":"Diagnostics"},{"issue":"7","key":"52613_CR15","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1089\/fpd.2017.2410","volume":"15","author":"J Gong","year":"2018","unstructured":"Gong, J. et al. Establishment of a Multiplex Loop-Mediated Isothermal Amplification Method for Rapid Detection of Sulfonamide Resistance Genes (sul1, sul2, sul3) in Clinical Enterobacteriaceae Isolates from Poultry. Foodborne Pathog Dis. 15 (7), 413\u2013419. https:\/\/doi.org\/10.1089\/fpd.2017.2410 (2018).","journal-title":"Foodborne Pathog Dis."},{"key":"52613_CR16","doi-asserted-by":"publisher","first-page":"8448","DOI":"10.1038\/s41598-020-64612","volume":"10","author":"J Rodriguez-Manzano","year":"2020","unstructured":"Rodriguez-Manzano, J. et al. Rapid Detection of Mobilized Colistin Resistance using a Nucleic Acid Based Lab-on-a-Chip Diagnostic System. Sci. Rep. 10, 8448. https:\/\/doi.org\/10.1038\/s41598-020-64612 (2020).","journal-title":"Sci. Rep."},{"key":"52613_CR17","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.3389\/fmicb.2020.01290","volume":"11","author":"W Xu","year":"2020","unstructured":"Xu, W. et al. Molecular Mechanisms and Epidemiology of Fosfomycin Resistance in Staphylococcus aureus Isolated From Patients at a Teaching Hospital in China. Front. Microbiol. 11, 1290. https:\/\/doi.org\/10.3389\/fmicb.2020.01290 (2020).","journal-title":"Front. Microbiol."},{"key":"52613_CR18","doi-asserted-by":"publisher","first-page":"1534084","DOI":"10.3389\/fcimb.2025.1534084","volume":"11","author":"R Zhang","year":"2025","unstructured":"Zhang, R. et al. Characterization of FosA13, a novel fosfomycin glutathione transferase identified in a Morganella morganii isolate from poultry. Front. Cell. Infect. Microbiol. 11, 15:1534084. https:\/\/doi.org\/10.3389\/fcimb.2025.1534084 (2025).","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"6","key":"52613_CR19","doi-asserted-by":"publisher","first-page":"107162","DOI":"10.1016\/j.ijantimicag.2024.107162","volume":"63","author":"Y Hong","year":"2024","unstructured":"Hong, Y. et al. Identification of the novel fosfomycin resistance gene fosSC in Staphylococcus capitis. Int. J. Antimicrob. 63 (6), 107162. https:\/\/doi.org\/10.1016\/j.ijantimicag.2024.107162 (2024).","journal-title":"Int. J. Antimicrob."},{"issue":"1","key":"52613_CR20","doi-asserted-by":"publisher","first-page":"29","DOI":"10.3390\/pathogens15010029","volume":"15","author":"W Goraj","year":"2026","unstructured":"Goraj, W. et al. Fosfomycin Resistance: An Update on the Anthropogenic Impact Through Agriculture. Pathogens 15 (1), 29. https:\/\/doi.org\/10.3390\/pathogens15010029 (2026).","journal-title":"Pathogens"},{"key":"52613_CR21","doi-asserted-by":"publisher","first-page":"914","DOI":"10.3389\/fmicb.2017.00914","volume":"19","author":"S Xu","year":"2017","unstructured":"Xu, S. et al. Mutations of the Transporter Proteins GlpT and UhpT Confer Fosfomycin Resistance in Staphylococcus aureus. Front. Microbiol. 19, 8:914. https:\/\/doi.org\/10.3389\/fmicb.2017.00914 (2017).","journal-title":"Front. Microbiol."},{"key":"52613_CR22","doi-asserted-by":"publisher","first-page":"1544","DOI":"10.3389\/fmicb.2015.01544","volume":"11","author":"Z Fu","year":"2016","unstructured":"Fu, Z. et al. Prevalence of Fosfomycin Resistance and Mutations in murA, glpT, and uhpT in Methicillin-Resistant Staphylococcus aureus Strains Isolated from Blood and Cerebrospinal Fluid Samples. Front. Microbiol. 11, 6:1544. https:\/\/doi.org\/10.3389\/fmicb.2015.01544 (2016).","journal-title":"Front. Microbiol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-52613-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-52613-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-52613-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T11:02:46Z","timestamp":1786446166000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-52613-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,30]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["52613"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-52613-5","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,30]]},"assertion":[{"value":"5 February 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 May 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"this article does not contain human or animal participants.","order":2,"name":"Ethics","label":"Ethics approval","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"24788"}}