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These fungi are responsible for superficial or invasive infections associated with high mortality. The aim of this study was to compare the activity of the new antifungal compound Venetin-1, obtained from the coelomic fluid of the earthworm\n                    <jats:italic>Dendrobaena veneta<\/jats:italic>\n                    , with the effects of the antibiotic amphotericin B (AmB). Flow cytometry, fluorescence microscopy, scanning electron microscopy, atomic force microscopy, and FTIR spectroscopic methods were used for comparative analysis. Venetin-1 at the highest concentration used (100\u00a0\u00b5g mL\n                    <jats:sup>\u2212\u20091<\/jats:sup>\n                    ) caused a decrease in the OD of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    cultures by more than 60% compared to the control group, and AmB (0.5\u00a0\u00b5g mL\n                    <jats:sup>\u2212\u20091<\/jats:sup>\n                    ) by 80%. Venetin-1 at the highest concentration reduced the viability of fungal cells by 42%, while AmB reduced it by 30%. The present study shows that both Venetin-1 and AmB induced fungal cell necrosis and apoptosis. SEM analysis showed that both compounds caused morphological changes in cells, including the presence of multiple bundles at the cell poles and the formation of unseparated cell chains. Congo red fluorochrome analysis showed that both compounds caused an increase in the fluorescence intensity of the \u03b2-glucan layer in the wall. Flow cytometric analysis of the cell wall showed that Venetin-1 caused exposure of the \u03b2-glucan and chitin layers in the fungal cells. FTIR analysis confirmed molecular remodelling of the cell wall structure after exposure to both compounds. AFM analysis indicated an increase in the elasticity of the fungal cell wall after treatment with both compounds. Proteomic analysis showed that amphotericin B and Venetin-1 induced distinct proteomic response patterns in\n                    <jats:italic>C. albicans<\/jats:italic>\n                    cells. AmB caused concentration-restricted response detectable at sublethal conditions, whereas Venetin-1 induced a concentration-dependent and progressively expanding proteomic remodelling. The comparison of Venetin-1 and AmB revealed pronounced differences in their electrokinetic behaviour, originating from their distinct chemical structures and modes of interaction with electrolyte ions. Venetin-1 exhibited significantly more negative zeta potential values, particularly in alkaline media and in the presence of Cl\u207b ions, indicating stronger electrostatic stabilisation and higher electrokinetic stability than AmB. Considering its effect on\n                    <jats:italic>Candida<\/jats:italic>\n                    cells and the fact that the Venetin-1 complex has no toxic effect, this compound is a promising candidate for further biomedical research on new antifungal preparations.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-52961-2","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T08:13:55Z","timestamp":1780128835000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Anti-Candida albicans effect of the new antifungal compound Venetin-1 obtained from earthworms versus amphotericin B"],"prefix":"10.1038","volume":"16","author":[{"given":"Kinga","family":"Lewtak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Dryglewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylwia","family":"Mieszawska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Skwarek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Czaplewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weronika","family":"\u015acibek-Rejmontowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcseyin","family":"Korkmaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerzy","family":"Wydrych","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Ka\u017amierski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Skrzypiec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta J.","family":"Fio\u0142ka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,30]]},"reference":[{"key":"52961_CR1","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1007\/BF02980222","volume":"30","author":"CK Lee","year":"2007","unstructured":"Lee, C. 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