{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T01:04:04Z","timestamp":1784077444820,"version":"3.55.0"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":61,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["25K10316"],"award-info":[{"award-number":["25K10316"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-026-53003-7","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T16:45:55Z","timestamp":1778863555000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Keratin 1 and keratin 18 as immunohistochemical markers for distinguishing pulmonary metastases of oral squamous cell carcinoma from primary lung squamous cell carcinoma"],"prefix":"10.1038","volume":"16","author":[{"given":"Yoshiko","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisami","family":"Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rei","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimio","family":"Yoshimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetaka","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayuri","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S M Jahurul","family":"Haque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiro","family":"Yoshimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Sugita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Takanashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keigo","family":"Sekihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhito","family":"Funai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuma","family":"Masumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuya","family":"Shinmura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"53003_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2024.107104","volume":"159","author":"F Esperouz","year":"2024","unstructured":"Esperouz, F. et al. Are we ready to use ultrasounds in the clinical assessment of depth of invasion and tumor thickness in oral squamous cell carcinoma? Results from a systematic review, meta-analysis and trial sequential analysis. Oral Oncol. 159, 107104. https:\/\/doi.org\/10.1016\/j.oraloncology.2024.107104 (2024).","journal-title":"Oral Oncol."},{"issue":"1","key":"53003_CR2","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-025-01863-2","volume":"19","author":"D Kumar","year":"2025","unstructured":"Kumar, D. et al. Association of epithelial to mesenchymal transition markers on prognosis and clinicopathological characteristics in oral squamous cell carcinoma: A systematic review and meta-analysis. Head Neck Pathol. 19(1), 124. https:\/\/doi.org\/10.1007\/s12105-025-01863-2 (2025).","journal-title":"Head Neck Pathol."},{"issue":"1","key":"53003_CR3","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-025-01839-2","volume":"19","author":"S Mp","year":"2025","unstructured":"Mp, S. et al. Micrometastasis and isolated tumor cells in oral squamous cell carcinoma: Refining nodal staging with emerging technologies. Head Neck Pathol. 19(1), 106. https:\/\/doi.org\/10.1007\/s12105-025-01839-2 (2025).","journal-title":"Head Neck Pathol."},{"key":"53003_CR4","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.713815","volume":"11","author":"HJ Lu","year":"2022","unstructured":"Lu, H. J. et al. Prediction model of distant metastasis in oral cavity squamous cell carcinoma with or without regional lymphatic metastasis. Front. Oncol. 11, 713815. https:\/\/doi.org\/10.3389\/fonc.2021.713815 (2022).","journal-title":"Front. Oncol."},{"key":"53003_CR5","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1001\/jamaoto.2016.3668","volume":"143","author":"A Hosni","year":"2017","unstructured":"Hosni, A. et al. Distant metastases following postoperative intensity-modulated radiotherapy for oral cavity squamous cell carcinoma. JAMA Otolaryngol. Head Neck Surg. 143, 368\u2013375. https:\/\/doi.org\/10.1001\/jamaoto.2016.3668 (2017).","journal-title":"JAMA Otolaryngol. Head Neck Surg."},{"key":"53003_CR6","doi-asserted-by":"publisher","first-page":"265","DOI":"10.4103\/2231-0762.186805","volume":"6","author":"S Irani","year":"2016","unstructured":"Irani, S. Distant metastasis from oral cancer: A review and molecular biologic aspects. J. Int. Soc. Prev. Community Dent. 6, 265\u2013271. https:\/\/doi.org\/10.4103\/2231-0762.186805 (2016).","journal-title":"J. Int. Soc. Prev. Community Dent."},{"key":"53003_CR7","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2025.107615","volume":"169","author":"PBS Marinho","year":"2025","unstructured":"Marinho, P. B. S. et al. Survival patterns and prognostic stratification based on distant metastasis characteristics in oral squamous cell carcinoma. Oral Oncol. 169, 107615. https:\/\/doi.org\/10.1016\/j.oraloncology.2025.107615 (2025).","journal-title":"Oral Oncol."},{"key":"53003_CR8","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/j.joms.2019.10.018","volume":"78","author":"S Sekikawa","year":"2020","unstructured":"Sekikawa, S. et al. Which factors affect the long-term survival of patients with oral squamous cell carcinoma with distant metastasis?. J. Oral Maxillofac. Surg. 78, 469\u2013478. https:\/\/doi.org\/10.1016\/j.joms.2019.10.018 (2020).","journal-title":"J. Oral Maxillofac. Surg."},{"key":"53003_CR9","doi-asserted-by":"publisher","DOI":"10.3390\/medicina58081000","volume":"58","author":"G Schlachtenberger","year":"2022","unstructured":"Schlachtenberger, G. et al. Patients with pulmonary metastases from head and neck cancer benefit from pulmonary metastasectomy: A systematic review. Medicina (Kaunas) 58, 1000. https:\/\/doi.org\/10.3390\/medicina58081000 (2022).","journal-title":"Medicina (Kaunas)"},{"key":"53003_CR10","doi-asserted-by":"publisher","first-page":"249","DOI":"10.6004\/jnccn.2204.0023","volume":"22","author":"GJ Riely","year":"2024","unstructured":"Riely, G. J. et al. Non-Small Cell Lung Cancer, Version 4.2024, NCCN Clinical Practice Guidelines in Oncology. J. Natl. Compr. Canc Netw. 22, 249\u2013274. https:\/\/doi.org\/10.6004\/jnccn.2204.0023 (2024).","journal-title":"J. Natl. Compr. Canc Netw."},{"key":"53003_CR11","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/j.ijom.2024.11.012","volume":"54","author":"H Kurita","year":"2025","unstructured":"Kurita, H. et al. Japanese clinical practice guidelines for oral cancer, 2023. Int. J. Oral Maxillofac. Surg. 54, 461\u2013476. https:\/\/doi.org\/10.1016\/j.ijom.2024.11.012 (2025).","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"53003_CR12","doi-asserted-by":"publisher","first-page":"29","DOI":"10.18332\/tid\/105844","volume":"17","author":"X Jiang","year":"2019","unstructured":"Jiang, X., Wu, J., Wang, J. & Huang, R. Tobacco and oral squamous cell carcinoma: A review of carcinogenic pathways. Tob. Induc. Dis. 17, 29. https:\/\/doi.org\/10.18332\/tid\/105844 (2019).","journal-title":"Tob. Induc. Dis."},{"key":"53003_CR13","doi-asserted-by":"publisher","first-page":"916","DOI":"10.1080\/0284186X.2022.2079958","volume":"61","author":"L\u00d8 Petersen","year":"2022","unstructured":"Petersen, L. \u00d8. et al. Second primary cancer following primary oral squamous cell carcinoma: A population-based, retrospective study. Acta Oncol. 61, 916\u2013921. https:\/\/doi.org\/10.1080\/0284186X.2022.2079958 (2022).","journal-title":"Acta Oncol."},{"key":"53003_CR14","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/j.jtho.2018.12.005","volume":"14","author":"Y Yatabe","year":"2019","unstructured":"Yatabe, Y. et al. Best practices recommendations for diagnostic immunohistochemistry in lung cancer. J. Thorac. Oncol. 14, 377\u2013407. https:\/\/doi.org\/10.1016\/j.jtho.2018.12.005 (2019).","journal-title":"J. Thorac. Oncol."},{"key":"53003_CR15","doi-asserted-by":"publisher","first-page":"384","DOI":"10.5858\/2008-132-384-AOITTD","volume":"132","author":"J Jagirdar","year":"2008","unstructured":"Jagirdar, J. Application of immunohistochemistry to the diagnosis of primary and metastatic carcinoma to the lung. Arch. Pathol. Lab. Med. 132, 384\u2013396. https:\/\/doi.org\/10.5858\/2008-132-384-AOITTD (2008).","journal-title":"Arch. Pathol. Lab. Med."},{"key":"53003_CR16","doi-asserted-by":"publisher","first-page":"972","DOI":"10.1093\/jnci\/90.13.972","volume":"90","author":"PP Leong","year":"1998","unstructured":"Leong, P. P. et al. Distinguishing second primary tumors from lung metastases in patients with head and neck squamous cell carcinoma. J. Natl. Cancer Inst. 90, 972\u2013977. https:\/\/doi.org\/10.1093\/jnci\/90.13.972 (1998).","journal-title":"J. Natl. Cancer Inst."},{"key":"53003_CR17","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1038\/s41568-018-0024-5","volume":"18","author":"A S\u00e1nchez-Dan\u00e9s","year":"2018","unstructured":"S\u00e1nchez-Dan\u00e9s, A. & Blanpain, C. Deciphering the cells of origin of squamous cell carcinomas. Nat. Rev. Cancer. 18, 549\u2013561. https:\/\/doi.org\/10.1038\/s41568-018-0024-5 (2018).","journal-title":"Nat. Rev. Cancer."},{"key":"53003_CR18","doi-asserted-by":"publisher","DOI":"10.1101\/mcs.a004317","volume":"5","author":"LM Sanders","year":"2019","unstructured":"Sanders, L. M. et al. Comparative RNA-seq analysis aids in diagnosis of a rare pediatric tumor. Cold. Spring. Harb. Mol. Case. Stud. 5, a004317. https:\/\/doi.org\/10.1101\/mcs.a004317 (2019).","journal-title":"Cold. Spring. Harb. Mol. Case. Stud."},{"key":"53003_CR19","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-49545-9","volume":"13","author":"H Goda","year":"2023","unstructured":"Goda, H. et al. KRT13 and UPK1B for differential diagnosis between metastatic lung carcinoma from oral squamous cell carcinoma and lung squamous cell carcinoma. Sci. Rep. 13, 22626. https:\/\/doi.org\/10.1038\/s41598-023-49545-9 (2023).","journal-title":"Sci. Rep."},{"key":"53003_CR20","doi-asserted-by":"publisher","first-page":"59312","DOI":"10.18632\/oncotarget.19576","volume":"8","author":"SW Mes","year":"2017","unstructured":"Mes, S. W. et al. Prognostic modeling of oral cancer by gene profiles and clinicopathological co-variables. Oncotarget 8, 59312\u201359323. https:\/\/doi.org\/10.18632\/oncotarget.19576 (2017).","journal-title":"Oncotarget"},{"key":"53003_CR21","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1038\/nature11404","volume":"489","author":"Cancer Genome Atlas Research Network","year":"2012","unstructured":"Cancer Genome Atlas Research Network. Comprehensive genomic characterization of squamous cell lung cancers. Nature 489, 519\u2013525. https:\/\/doi.org\/10.1038\/nature11404 (2012).","journal-title":"Nature"},{"key":"53003_CR22","doi-asserted-by":"publisher","first-page":"4348","DOI":"10.1016\/j.cell.2021.07.016","volume":"184","author":"S Satpathy","year":"2021","unstructured":"Satpathy, S. et al. A proteogenomic portrait of lung squamous cell carcinoma. Cell 184, 4348\u20134371e40. https:\/\/doi.org\/10.1016\/j.cell.2021.07.016 (2021).","journal-title":"Cell"},{"issue":"2","key":"53003_CR23","first-page":"427","volume":"140","author":"R Moll","year":"1992","unstructured":"Moll, R., L\u00f6we, A., Laufer, J. & Franke, W. W. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am. J. Pathol. 140(2), 427\u2013447 (1992).","journal-title":"Am. J. Pathol."},{"key":"53003_CR24","doi-asserted-by":"publisher","first-page":"1435","DOI":"10.1007\/s00405-021-06894-3","volume":"279","author":"RSR Woods","year":"2022","unstructured":"Woods, R. S. R. et al. Cytokeratin 7 and 19 expression in oropharyngeal and oral squamous cell carcinoma. Eur. Arch. Otorhinolaryngol. 279, 1435\u20131444. https:\/\/doi.org\/10.1007\/s00405-021-06894-3 (2022).","journal-title":"Eur. Arch. Otorhinolaryngol."},{"key":"53003_CR25","doi-asserted-by":"publisher","first-page":"1332","DOI":"10.3390\/diagnostics12061332","volume":"12","author":"V Kumar","year":"2022","unstructured":"Kumar, V. et al. Evaluation of Heterogeneous Nuclear Ribonucleoprotein D Expression as a Diagnostic Marker for Oral Squamous Cell Carcinoma. Diagnostics (Basel). 12, 1332. https:\/\/doi.org\/10.3390\/diagnostics12061332 (2022).","journal-title":"Diagnostics (Basel)"},{"key":"53003_CR26","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1111\/odi.13791","volume":"28","author":"F Yin","year":"2022","unstructured":"Yin, F. et al. Activation of EGFR-Aurora A induces loss of primary cilia in oral squamous cell carcinoma. Oral Dis. 28, 621\u2013630. https:\/\/doi.org\/10.1111\/odi.13791 (2022).","journal-title":"Oral Dis."},{"key":"53003_CR27","doi-asserted-by":"publisher","first-page":"100456","DOI":"10.1016\/j.ctarc.2021.100456","volume":"29","author":"Y Osawa","year":"2021","unstructured":"Osawa, Y. et al. Somatic mutations in oral squamous cell carcinomas in 98 Japanese patients and their clinical implications. Cancer Treat. Res. Commun. 29, 100456. https:\/\/doi.org\/10.1016\/j.ctarc.2021.100456 (2021).","journal-title":"Cancer Treat. Res. Commun."},{"key":"53003_CR28","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1007\/s00418-008-0435-6","volume":"129","author":"R Moll","year":"2008","unstructured":"Moll, R., Divo, M. & Langbein, L. The human keratins: biology and pathology. Histochem. Cell. Biol. 129, 705\u2013733. https:\/\/doi.org\/10.1007\/s00418-008-0435-6 (2008).","journal-title":"Histochem. Cell. Biol."},{"key":"53003_CR29","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/s1368-8375(00)00094-4","volume":"37","author":"BK Bloor","year":"2001","unstructured":"Bloor, B. K., Seddon, S. V. & Morgan, P. R. Gene expression of differentiation-specific keratins in oral epithelial dysplasia and squamous cell carcinoma. Oral Oncol. 37, 251\u2013261. https:\/\/doi.org\/10.1016\/s1368-8375(00)00094-4 (2001).","journal-title":"Oral Oncol."},{"key":"53003_CR30","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/s10020-021-00274-7","volume":"27","author":"A Menz","year":"2021","unstructured":"Menz, A. et al. Diagnostic and prognostic impact of cytokeratin 18 expression in human tumors: a tissue microarray study on 11,952 tumors. Mol. Med. 27, 16. https:\/\/doi.org\/10.1186\/s10020-021-00274-7 (2021).","journal-title":"Mol. Med."},{"key":"53003_CR31","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1111\/apm.13280","volume":"131","author":"M Lennartz","year":"2023","unstructured":"Lennartz, M. et al. Cytokeratin 13 (CK13) expression in cancer: a tissue microarray study on 10,439 tumors. APMIS 131, 77\u201391. https:\/\/doi.org\/10.1111\/apm.13280 (2023).","journal-title":"APMIS"},{"key":"53003_CR32","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1016\/j.athoracsur.2018.10.028","volume":"107","author":"JR Handy","year":"2019","unstructured":"Handy, J. R. et al. Expert Consensus Document on Pulmonary Metastasectomy. Ann. Thorac. Surg. 107, 631\u2013649. https:\/\/doi.org\/10.1016\/j.athoracsur.2018.10.028 (2019).","journal-title":"Ann. Thorac. Surg."},{"key":"53003_CR33","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1200\/JCO.22.00051","volume":"40","author":"K Pisters","year":"2022","unstructured":"Pisters, K., Kris, M. G., Gaspar, L. E., Ismaila, N., Adjuvant Systemic Therapy and Adjuvant Radiation Therapy for Stage I to IIIA NSCLC Guideline Expert Panel. Adjuvant systemic therapy and adjuvant radiation therapy for Stage I-IIIA completely resected non-small-cell lung cancer: ASCO guideline rapid recommendation update. J. Clin. Oncol. 40, 1127\u20131129. https:\/\/doi.org\/10.1200\/JCO.22.00051 (2022).","journal-title":"J. Clin. Oncol."},{"key":"53003_CR34","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1002\/path.2953","volume":"225","author":"K Shinmura","year":"2011","unstructured":"Shinmura, K. et al. Reduced expression of MUTYH with suppressive activity against mutations caused by 8-hydroxyguanine is a novel predictor of a poor prognosis in human gastric cancer. J. Pathol. 225, 414\u2013423. https:\/\/doi.org\/10.1002\/path.2953 (2011).","journal-title":"J. Pathol."},{"key":"53003_CR35","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7365913","volume":"2019","author":"Y Dong","year":"2019","unstructured":"Dong, Y. et al. Myoferlin, a membrane protein with emerging oncogenic roles. Biomed. Res. Int. 2019, 7365913. https:\/\/doi.org\/10.1155\/2019\/7365913 (2019).","journal-title":"Biomed. Res. Int."},{"key":"53003_CR36","doi-asserted-by":"publisher","DOI":"10.1186\/s12957-023-03102-w","volume":"21","author":"H Xing","year":"2023","unstructured":"Xing, H. et al. High expression of RPL27A predicts poor prognosis in patients with hepatocellular carcinoma. World J. Surg. Oncol. 21, 209. https:\/\/doi.org\/10.1186\/s12957-023-03102-w (2023).","journal-title":"World J. Surg. Oncol."},{"key":"53003_CR37","doi-asserted-by":"publisher","first-page":"33","DOI":"10.4132\/jptm.2020.09.29","volume":"55","author":"T Jeon","year":"2021","unstructured":"Jeon, T., Kim, A. & Kim, C. Automated immunohistochemical assessment ability to evaluate estrogen and progesterone receptor status compared with quantitative reverse transcription-polymerase chain reaction in breast carcinoma patients. J. Pathol. Transl. Med. 55, 33\u201342. https:\/\/doi.org\/10.4132\/jptm.2020.09.29 (2021).","journal-title":"J. Pathol. Transl. Med."},{"key":"53003_CR38","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1016\/j.pathol.2016.05.008","volume":"48","author":"MZ Leivo","year":"2016","unstructured":"Leivo, M. Z., Elson, P. J., Tacha, D. E., Delahunt, B. & Hansel, D. E. A combination of p40, GATA-3 and uroplakin II shows utility in the diagnosis and prognosis of muscle-invasive urothelial carcinoma. Pathology 48, 543\u2013549. https:\/\/doi.org\/10.1016\/j.pathol.2016.05.008 (2016).","journal-title":"Pathology"},{"key":"53003_CR39","doi-asserted-by":"publisher","first-page":"129","DOI":"10.4103\/sjg.SJG_465_17","volume":"24","author":"J Al-Maghrabi","year":"2018","unstructured":"Al-Maghrabi, J., Emam, E. & Gomaa, W. Immunohistochemical staining of cytokeratin 20 and cytokeratin 7 in colorectal carcinomas: Four different immunostaining profiles. Saudi J. Gastroenterol. 24, 129\u2013134. https:\/\/doi.org\/10.4103\/sjg.SJG_465_17 (2018).","journal-title":"Saudi J. Gastroenterol."},{"key":"53003_CR40","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201846589","volume":"19","author":"T Eguether","year":"2018","unstructured":"Eguether, T. & Hahne, M. Mixed signals from the cell\u2019s antennae: Primary cilia in cancer. EMBO Rep. 19, e46589. https:\/\/doi.org\/10.15252\/embr.201846589 (2018).","journal-title":"EMBO Rep."},{"key":"53003_CR41","doi-asserted-by":"publisher","DOI":"10.1016\/j.labinv.2022.100007","volume":"103","author":"K Shinmura","year":"2023","unstructured":"Shinmura, K. et al. Primary cilia are frequently present in small cell lung carcinomas but not in non-small cell lung carcinomas or lung carcinoids. Lab. Invest. 103, 100007. https:\/\/doi.org\/10.1016\/j.labinv.2022.100007 (2023).","journal-title":"Lab. Invest."},{"key":"53003_CR42","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1111\/j.1600-0463.2003.apm1111201.x","volume":"111","author":"T Tot","year":"2003","unstructured":"Tot, T. & Samii, S. The clinical relevance of cytokeratin phenotyping in needle biopsy of liver metastasis. APMIS 111, 1075\u20131082. https:\/\/doi.org\/10.1111\/j.1600-0463.2003.apm1111201.x (2003).","journal-title":"APMIS"},{"key":"53003_CR43","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1002\/path.5688","volume":"254","author":"K Shinmura","year":"2021","unstructured":"Shinmura, K. et al. Identification and characterization of primary cilia-positive salivary gland tumours exhibiting basaloid\/myoepithelial differentiation. J. Pathol. 254, 519\u2013530. https:\/\/doi.org\/10.1002\/path.5688 (2021).","journal-title":"J. Pathol."},{"key":"53003_CR44","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","volume":"34","author":"S Chen","year":"2018","unstructured":"Chen, S., Zhou, Y., Chen, Y. & Gu, J. Fastp: An ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34, i884\u2013890. https:\/\/doi.org\/10.1093\/bioinformatics\/bty560 (2018).","journal-title":"Bioinformatics"},{"key":"53003_CR45","doi-asserted-by":"publisher","first-page":"D754","DOI":"10.1093\/nar\/gkx1098","volume":"46","author":"DR Zerbino","year":"2018","unstructured":"Zerbino, D. R. et al. Ensembl 2018. Nucleic Acids Res. 46, D754\u2013761. https:\/\/doi.org\/10.1093\/nar\/gkx1098 (2018).","journal-title":"Nucleic Acids Res."},{"key":"53003_CR46","doi-asserted-by":"publisher","unstructured":"Vasimuddin, M., Misra, S., Li, H. & Aluru, S. Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems. Proc. IEEE IPDPS. 314\u2013324. https:\/\/doi.org\/10.1109\/ipdps.2019.00041 (2019).","DOI":"10.1109\/ipdps.2019.00041"},{"key":"53003_CR47","doi-asserted-by":"publisher","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","volume":"25","author":"1000 Genome Project Data Processing Subgroup","year":"2009","unstructured":"1000 Genome Project Data Processing Subgroup. The Sequence Alignment\/Map format and SAMtools. Bioinformatics 25, 2078\u20132079. https:\/\/doi.org\/10.1093\/bioinformatics\/btp352 (2009).","journal-title":"Bioinformatics"},{"key":"53003_CR48","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1101\/gr.107524.110","volume":"20","author":"A McKenna","year":"2010","unstructured":"McKenna, A. et al. The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 20, 1297\u20131303. https:\/\/doi.org\/10.1101\/gr.107524.110 (2010).","journal-title":"Genome Res."},{"key":"53003_CR49","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1038\/s41592-018-0051-x","volume":"15","author":"S Kim","year":"2018","unstructured":"Kim, S. et al. Strelka2: fast and accurate calling of germline and somatic variants. Nat. Methods. 15, 591\u2013594. https:\/\/doi.org\/10.1038\/s41592-018-0051-x (2018).","journal-title":"Nat. Methods"},{"key":"53003_CR50","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1101\/gr.129684.111","volume":"22","author":"DC Koboldt","year":"2012","unstructured":"Koboldt, D. C. et al. VarScan 2: somatic mutation and copy number alteration discovery in cancer by exome sequencing. Genome Res. 22, 568\u2013576. https:\/\/doi.org\/10.1101\/gr.129684.111 (2012).","journal-title":"Genome Res."},{"key":"53003_CR51","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq603","volume":"38","author":"K Wang","year":"2010","unstructured":"Wang, K., Li, M. & Hakonarson, H. ANNOVAR: Functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Res. 38, e164. https:\/\/doi.org\/10.1093\/nar\/gkq603 (2010).","journal-title":"Nucleic Acids Res."},{"key":"53003_CR52","doi-asserted-by":"publisher","first-page":"1536","DOI":"10.1093\/bioinformatics\/btv009","volume":"31","author":"HA Shihab","year":"2015","unstructured":"Shihab, H. A. et al. An integrative approach to predicting the functional effects of non-coding and coding sequence variation. Bioinformatics 31, 1536\u20131543. https:\/\/doi.org\/10.1093\/bioinformatics\/btv009 (2015).","journal-title":"Bioinformatics"},{"key":"53003_CR53","volume-title":"R: a language and environment for statistical computing","author":"R Core Team","year":"2024","unstructured":"R Core Team. R: a language and environment for statistical computing (R Foundation for Statistical Computing, 2024)."},{"key":"53003_CR54","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-77","volume":"12","author":"X Robin","year":"2011","unstructured":"Robin, X. et al. pROC: An open-source package for R and S\u2009+\u2009to analyze and compare ROC curves. BMC Bioinformatics 12, 77. https:\/\/doi.org\/10.1186\/1471-2105-12-77 (2011).","journal-title":"BMC Bioinformatics"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-53003-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-53003-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-53003-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:04:19Z","timestamp":1784073859000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-53003-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,15]]},"references-count":54,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["53003"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-53003-7","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,15]]},"assertion":[{"value":"29 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 May 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Ethics"}},{"value":"This study was approved by the Clinical Research Review Board of Hamamatsu University School of Medicine (approval no. 24\u2013242). The requirement for informed consent was waived.","order":2,"name":"Ethics","label":"Ethics Approval","group":{"name":"EthicsHeading","label":"Ethics"}}],"article-number":"22187"}}