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Subsequently, employing the BPH-1 cell line in vitro, we preliminarily investigated the potential biological functions of the prioritized epithelial target, ANXA2, through siRNA-mediated knockdown, Western blotting, ELISA, and cell proliferation assays. We computationally identified ANXA2 and IFI27 as key lactylation-related candidate genes, both of which were significantly downregulated in BPH tissues. scRNA-seq data revealed their cell-type specificity, demonstrating that ANXA2 is predominantly expressed in epithelial cells, whereas IFI27 is primarily expressed in endothelial cells. Furthermore, we uncovered a potential link between metabolism and inflammation involving ANXA2. Specifically, ANXA2 knockdown in BPH-1 cells was associated with alterations in glycolytic gene expression and a concomitant reduction in lactate production. Our data suggest that this decreased lactate level is associated with elevated secretion of the proinflammatory cytokine IL-6 and enhanced cellular proliferation. Additionally, cellular trajectory and communication analyses indicated that ANXA2\u207a epithelial cells and IFI27\u207a endothelial cells represent distinct subpopulations characterized by enhanced intercellular signaling capacities, suggesting their potential roles as critical molecular hubs within the BPH microenvironment. This study presents the first single-cell resolution landscape of lactylation-related gene activity in BPH. We computationally identified ANXA2 and IFI27 as key lactylation-related candidate genes. Importantly, our study suggests a potential link whereby ANXA2 deficiency is associated with BPH cell proliferation and potential metabolic and inflammatory alterations. These findings provide valuable new insights into the underlying pathophysiological mechanisms of BPH and lay the groundwork for the development of targeted therapeutic strategies.<\/jats:p>","DOI":"10.1038\/s41598-026-54516-x","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T07:24:51Z","timestamp":1779693891000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrating single-cell and bulk transcriptomic analyses to explore key lactylation-related genes in benign prostatic hyperplasia"],"prefix":"10.1038","volume":"16","author":[{"given":"Xiaotong","family":"Pang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyong","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongbin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fubo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"issue":"4","key":"54516_CR1","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1111\/bju.15229","volume":"127","author":"CM Devlin","year":"2021","unstructured":"Devlin, C. 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The study protocol strictly adhered to the principles of the Declaration of Helsinki and was reviewed and approved by the Medical Ethics Committee of the Second People\u2019s Hospital of Nanning (Approval No.: Y2025111). All specimens were collected after obtaining written informed consent from the participants.","order":2,"name":"Ethics","label":"Ethics statement","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"23814"}}