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Inadequate surveillance and diagnostic resources in low- and middle-income countries impede its effective public health responses. This study demonstrates the use of an extreme gradient boosting (XGB) technique for animal rabies risk assessment in endemic areas of Haiti with limited diagnostic resources. We employed spatiotemporal clustering and trend analysis techniques to assess rabies status across large geographic areas. The XGB model achieved high specificity (0.99, 95% CI: 0.98\u20130.99), sensitivity (0.78, 95% CI: 0.61\u20130.95), negative predictive value (1.0, 95% CI: 0.99\u20131.00), and accuracy (0.98, 95% CI: 0.98\u20130.99) in predicting animal rabies cases. The framework identified 20 high-risk rabies clusters, representing a 40% increase in detected transmission zones compared to laboratory-confirmed data alone. This included 8 clusters in underserved communities where no diagnostic infrastructure was previously available, enabling real-time monitoring of disease and surveillance trends in previously unmonitored regions. Trend analysis facilitated real-time monitoring of high-risk clusters with significantly changing disease and surveillance statuses. This study showcases a data-driven, evidence-based framework for neglected and emerging zoonotic disease surveillance in economically constrained regions.<\/jats:p>","DOI":"10.1038\/s41598-026-55346-7","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T06:44:30Z","timestamp":1779950670000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biosurveillance and early outbreak detection of rabies in settings with limited laboratory capacity using spatiotemporal clustering and a machine learning framework"],"prefix":"10.1038","volume":"16","author":[{"given":"Ravikiran","family":"Keshavamurthy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse","family":"Blanton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillian","family":"Orciari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Gibson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Dilius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haim","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan M.","family":"Wallace","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"55346_CR1","doi-asserted-by":"publisher","unstructured":"WHO Rabies Modelling Consortium. 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Using Integrated Bite Case Management to estimate the burden of rabies and evaluate surveillance in Oriental Mindoro, Philippines. One health & Implement. research 3, https:\/\/doi.org\/10.20517\/ohir.2023.02 (2023).","DOI":"10.20517\/ohir.2023.02"},{"key":"55346_CR5","doi-asserted-by":"publisher","unstructured":"Castillo-Neyra, R. et al. Spatial Association of Canine Rabies Outbreak and Ecological Urban Corridors, Arequipa, Peru. Tropical Med. Infect. Disease 2017, Vol. 2, Page 38 2, https:\/\/doi.org\/10.3390\/tropicalmed2030038 (2017).","DOI":"10.3390\/tropicalmed2030038"},{"key":"55346_CR6","doi-asserted-by":"publisher","unstructured":"Ma, X. et al. Quantifying the risk of rabies in biting dogs in Haiti. Scientific Reports 10, (2020). https:\/\/doi.org\/10.1038\/s41598-020-57908-9 (2020).","DOI":"10.1038\/s41598-020-57908-9"},{"key":"55346_CR7","doi-asserted-by":"publisher","unstructured":"Medley, A. M. et al. 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This study involving human participants was reviewed and approved by the CDC as not to be research, as the purpose of these activities was to investigate and assess a condition of public health importance. CDC Institutional Review Board (IRB) review was not required under the following provision: Public health surveillance activities, 45 CFR 46.102(l)(2). Written informed consent for participation was not required for this study in accordance with the Minist\u00e8re de la Sant\u00e9 Publique et de la Population (MSPP) in Haiti and the IRB at the U.S. CDC (Public health surveillance activities, 45 CFR 46.102(l)(2)). The animal study was reviewed and approved by CDC Institutional Animal Care and Use Committee (IACUC). Written informed consent for participation was not obtained from the owners because the purpose of this surveillance program is to monitor the occurrence of animal rabies cases in Haiti through the investigation of animal bites and through the testing of pathology samples from deceased animals. The surveillance program is a component of the national rabies control program, which includes vaccination programs, PEP guidelines, and animal quarantine and euthanasia policies. Surveillance will be used to inform ongoing practice within the national rabies control program. As an activity designed to monitor the occurrence of disease in a defined population, as well as to provide feedback to inform ongoing public health practice, this activity is consistent with the attributes of non-research public health practice, as described in current CDC policy.","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"24391"}}