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The MNPs coated with oleic acid (OA) were synthesized via co-precipitation, resulting in oleic acid-coated magnetite NPs (OAMNPs). These were combined with Poly(lactic-co-glycolic acid) (PLGA) and DPZ to create the nanohybrid, OAMNPs-PLGA-DPZ. The nanohybrid has been characterized utilizing Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Transmission electron microscopy (TEM). Drug loading capacity (LC%) and encapsulation efficiency (EE%) were quantified by Ultraviolet-Visible Spectrophotometry (UV-Vis), and\n                    <jats:italic>in\u2011vitro<\/jats:italic>\n                    drug release was evaluated at physiological pH 7.4. Finally, the cytotoxicity of OAMNPs-PLGA-DPZ on SH-SY5Y cells was determined using an MTT assay. The crystalline size of OAMNPs-PLGA-DPZ, as revealed by XRD, ranged from 20 to 60\u00a0nm, and the wurtzite crystal structure size was 12.95\u00a0nm. The FTIR spectra showed that DPZ was successfully encapsulated within the PLGA matrix, and the TEM images revealed the synthesis and production of OAMNP-PLGA-DPZ. Biocompatibility evaluation demonstrated an IC\u2085\u2080 of 13.5\u2009\u00b1\u20092.12\u00a0\u00b5g\/mL as a baseline for the safety profile after 48\u00a0h of exposure. Taken together, these findings suggested that OAMNPs\u2011PLGA\u2011DPZ represent a foundational drug\u2011delivery platform with potential applications for neurodegenerative diseases.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-59963-0","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:14:35Z","timestamp":1783689275000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis, characterization, and biocompatibility evaluation of donepezil-loaded magnetite-PLGA nanoparticles in neuroblastoma cells"],"prefix":"10.1038","volume":"16","author":[{"given":"Norazalina","family":"Saad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Che Azurahanim Che","family":"Abdullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norhaizan Mohd","family":"Esa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmellie Laura","family":"Albert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abubakar Dantani","family":"Meli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Abdelmonem","family":"Abdelsamea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"59963_CR1","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1038\/s41582-019-0244-7","volume":"15","author":"Y Hou","year":"2019","unstructured":"Hou, Y. et al. 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