{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:42:25Z","timestamp":1792453345618,"version":"4.5.0"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":93,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"The university-level \"Peak Building and Spire Creation\" project"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-026-60535-5","type":"journal-article","created":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T08:23:19Z","timestamp":1784449399000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unraveling the therapeutic mechanisms of placenta hominis for polycystic ovary syndrome through network pharmacology, molecular docking, and experimental validation"],"prefix":"10.1038","volume":"16","author":[{"given":"Haixia","family":"Pan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meilin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingqiao","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daopeng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,19]]},"reference":[{"issue":"10","key":"60535_CR1","doi-asserted-by":"publisher","first-page":"2447","DOI":"10.1210\/clinem\/dgad463","volume":"108","author":"HJ Teede","year":"2023","unstructured":"Teede, H. J. et al. Recommendations from the 2023 international evidence-based guideline for the assessment and management of polycystic ovary syndrome. J. Clin. Endocrinol. Metab. 108 (10), 2447\u20132469 (2023).","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"60535_CR2","unstructured":"Shibin, C., Feng, Z., Yihan, W., Zhilai, Z. & Qing, M. Herbal textual research on hominis placenta in famous classical formulas. Chin. J. Exp. Tradit. Med. Form. 1\u201316 (2025)."},{"issue":"18","key":"60535_CR3","first-page":"38","volume":"34","author":"H Lanmei","year":"2025","unstructured":"Lanmei, H., Jun, Z. & Lin, Y. Improvement effect of human placenta on premature ovarian insufficiency in model mice. China Pharmaceut. 34 (18), 38\u201341 (2025).","journal-title":"China Pharmaceut."},{"issue":"11","key":"60535_CR4","first-page":"232","volume":"42","author":"Q Lu","year":"2024","unstructured":"Lu, Q. & Lin, N. Research progress on the active components, pharmacological effects and clinical applications of placenta hominis. Chin. Arch. Tradit. Chin. Med. 42 (11), 232\u2013236 (2024).","journal-title":"Chin. Arch. Tradit. Chin. Med."},{"key":"60535_CR5","unstructured":"Baozhen Peng, H. Z. A prospective study of 59 cases of strengthening the kidney function and promoting ovulation. Shaanxi Tradit. Chin. Med. 11, 488 (1997)."},{"issue":"04","key":"60535_CR6","first-page":"118","volume":"28","author":"Y Liu","year":"2011","unstructured":"Liu, Y. & Xi, L. A discussion on the use of zihetao capsules in treating infertility caused by insufficient uterine endometrium. Inf. Tradit. Chin. Med. 28 (04), 118\u2013119 (2011).","journal-title":"Inf. Tradit. Chin. Med."},{"issue":"01","key":"60535_CR7","first-page":"61","volume":"22","author":"H Tang","year":"2010","unstructured":"Tang, H. Experience in treating 62 cases of infertility caused by uterine dysplasia with the formula for tonifying the kidney and regulating the menstruation. J. Clin. Chin. Med. 22 (01), 61 (2010).","journal-title":"J. Clin. Chin. Med."},{"issue":"10","key":"60535_CR8","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1038\/nbt1338","volume":"25","author":"MA Yildirim","year":"2007","unstructured":"Yildirim, M. A., Goh, K., Cusick, M. E., Barabasi, A. & Vidal, M. Drug-target network. Nat. Biotechnol. 25 (10), 1119\u20131126 (2007).","journal-title":"Nat. Biotechnol."},{"key":"60535_CR9","doi-asserted-by":"publisher","first-page":"153","DOI":"10.2147\/IJWH.S490836","volume":"17","author":"B Jiang","year":"2025","unstructured":"Jiang, B. The global burden of polycystic ovary syndrome in women of reproductive age: Findings from the GBD 2019 study. Int. J. Womens Health 17, 153\u2013165 (2025).","journal-title":"Int. J. Womens Health"},{"issue":"19","key":"60535_CR10","doi-asserted-by":"publisher","first-page":"9702","DOI":"10.3390\/ijms26199702","volume":"26","author":"D Koleva-Tyutyundzhieva","year":"2025","unstructured":"Koleva-Tyutyundzhieva, D., Ilieva-Gerova, M., Deneva, T. & Orbetzova, M. Metabolic and inflammatory adipokine profiles in PCOS: A focus on adiposity, insulin resistance, and atherogenic risk. Int. J. Mol. Sci. 26 (19), 9702 (2025).","journal-title":"Int. J. Mol. Sci."},{"key":"60535_CR11","doi-asserted-by":"publisher","first-page":"117083","DOI":"10.1016\/j.bcp.2025.117083","volume":"239","author":"X Li","year":"2025","unstructured":"Li, X. et al. Isatin ameliorates ovarian inflammation and apoptosis caused by hormonal imbalances in PCOS mice. Biochem. Pharmacol. 239, 117083 (2025).","journal-title":"Biochem. Pharmacol."},{"key":"60535_CR12","doi-asserted-by":"publisher","first-page":"3853","DOI":"10.2147\/DDDT.S456656","volume":"18","author":"R Zhang","year":"2024","unstructured":"Zhang, R. et al. Mechanism of yjkl decoction in treating of pcos infertility by integrative approach of network pharmacology and experimental verification. Drug Des. Dev. Ther. 18, 3853\u20133870 (2024).","journal-title":"Drug Des. Dev. Ther."},{"key":"60535_CR13","doi-asserted-by":"publisher","first-page":"1455964","DOI":"10.3389\/fmed.2024.1455964","volume":"11","author":"J Miao","year":"2024","unstructured":"Miao, J. et al. Exploring the therapeutic mechanisms of Yikang decoction in polycystic ovary syndrome: An integration of geo datasets, network pharmacology, and molecular dynamics simulations. Front. Med. 11, 1455964 (2024).","journal-title":"Front. Med."},{"issue":"Pt 3","key":"60535_CR14","doi-asserted-by":"publisher","first-page":"117346","DOI":"10.1016\/j.jep.2023.117346","volume":"319","author":"Y Liu","year":"2024","unstructured":"Liu, Y. et al. Mechanism of cryptotanshinone to improve endocrine and metabolic functions in the endometrium of PCOS rats. J. Ethnopharmacol. 319 (Pt 3), 117346 (2024).","journal-title":"J. Ethnopharmacol."},{"issue":"15","key":"60535_CR15","doi-asserted-by":"publisher","first-page":"3822","DOI":"10.3390\/ijms20153822","volume":"20","author":"RM Marquardt","year":"2019","unstructured":"Marquardt, R. M., Kim, T. H., Shin, J. & Jeong, J. Progesterone and estrogen signaling in the endometrium: What goes wrong in endometriosis? Int. J. Mol. Sci. 20 (15), 3822 (2019).","journal-title":"Int. J. Mol. Sci."},{"issue":"20","key":"60535_CR16","doi-asserted-by":"publisher","first-page":"e71141","DOI":"10.1096\/fj.202502357R","volume":"39","author":"M Sui","year":"2025","unstructured":"Sui, M., Wu, S., Song, Q., Chen, S. & Lu, Y. Foxo6, stabilized by mettl3-mediated m6a modification, accelerates polycystic ovary syndrome progression by transcriptional activation of txnip. FASEB J. 39 (20), e71141 (2025).","journal-title":"FASEB J."},{"key":"60535_CR17","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.omtn.2020.02.007","volume":"20","author":"G Liu","year":"2020","unstructured":"Liu, G., Liu, S., Xing, G. & Wang, F. Lncrna pvt1\/microrna-17-5p\/pten axis regulates secretion of e2 and p4, proliferation, and apoptosis of ovarian granulosa cells in pcos. Mol. Ther. Nucleic Acids. 20, 205\u2013216 (2020).","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"60535_CR18","doi-asserted-by":"publisher","first-page":"123","DOI":"10.3389\/fphar.2019.00123","volume":"10","author":"R Zhang","year":"2019","unstructured":"Zhang, R., Zhu, X., Bai, H. & Ning, K. Network pharmacology databases for traditional Chinese medicine: Review and assessment. Front. Pharmacol. 10, 123 (2019).","journal-title":"Front. Pharmacol."},{"issue":"33","key":"60535_CR19","first-page":"129","volume":"21","author":"C Guo","year":"2024","unstructured":"Guo, C. Effect of ziheche powder combined with dydrogesterone on patients with threatened abortion. Med. Innov. China. 21 (33), 129\u2013133 (2024).","journal-title":"Med. Innov. China"},{"issue":"18","key":"60535_CR20","first-page":"38","volume":"34","author":"JZL Lanmei Huang","year":"2025","unstructured":"Lanmei Huang, J. Z. L. Improvement effect of human placenta on premature ovarian insufficiency in model mice. China Pharmaceut. 34 (18), 38\u201341 (2025).","journal-title":"China Pharmaceut."},{"key":"60535_CR21","doi-asserted-by":"publisher","first-page":"156852","DOI":"10.1016\/j.phymed.2025.156852","volume":"143","author":"J Zhang","year":"2025","unstructured":"Zhang, J. et al. Erchen decoction ameliorates the rat model of polycystic ovary syndrome by regulating the steroid biosynthesis pathway. Phytomedicine 143, 156852 (2025).","journal-title":"Phytomedicine"},{"issue":"1","key":"60535_CR22","doi-asserted-by":"publisher","first-page":"2181637","DOI":"10.1080\/09513590.2023.2181637","volume":"39","author":"Y Zhang","year":"2023","unstructured":"Zhang, Y., Zhou, H. & Ding, C. The ameliorative effect of Cangfu Daotan Decoction on polycystic ovary syndrome of rodent model is associated with m6a methylation and wnt\/beta-catenin pathway. Gynecol. Endocrinol. 39 (1), 2181637 (2023).","journal-title":"Gynecol. Endocrinol."},{"issue":"1","key":"60535_CR23","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1186\/s13048-024-01552-8","volume":"17","author":"X Xin","year":"2024","unstructured":"Xin, X. et al. Exploring lncrna expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high-throughput sequencing technology. J. Ovarian Res. 17 (1), 220 (2024).","journal-title":"J. Ovarian Res."},{"issue":"1","key":"60535_CR24","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1210\/endo.142.1.7862","volume":"142","author":"Y Nishi","year":"2001","unstructured":"Nishi, Y. et al. Establishment and characterization of a steroidogenic human granulosa-like tumor cell line, KGN, that expresses functional follicle-stimulating hormone receptor. Endocrinology 142 (1), 437\u2013445 (2001).","journal-title":"Endocrinology"},{"key":"60535_CR25","doi-asserted-by":"publisher","first-page":"109094","DOI":"10.1016\/j.abb.2021.109094","volume":"715","author":"R Ji","year":"2022","unstructured":"Ji, R. et al. Salidroside alleviates oxidative stress and apoptosis via AMPK\/Nrf2 pathway in DHT-induced human granulosa cell line KGN. Arch. Biochem. Biophys. 715, 109094 (2022).","journal-title":"Arch. Biochem. Biophys."},{"issue":"12","key":"60535_CR26","doi-asserted-by":"publisher","first-page":"2432","DOI":"10.1038\/s41401-023-01128-0","volume":"44","author":"H Ye","year":"2023","unstructured":"Ye, H. et al. Serum granulosa cell-derived TNF-\u03b1 promotes inflammation and apoptosis of renal tubular cells and PCOS-related kidney injury through NF-\u03baB signaling. Acta Pharmacol. Sin. 44 (12), 2432\u20132444 (2023).","journal-title":"Acta Pharmacol. Sin"},{"key":"60535_CR27","doi-asserted-by":"crossref","unstructured":"Liu, S., Sun, Y., Li, J., Dong, J. & Bian, Q. Preparation of herbal medicine: Er-Xian decoction and Er-Xian-containing serum for in vivo and in vitro experiments. J. Vis. Exp. 123 (2017).","DOI":"10.3791\/55654"},{"issue":"3","key":"60535_CR28","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1096\/fj.07-9574LSF","volume":"22","author":"S Reagan-Shaw","year":"2008","unstructured":"Reagan-Shaw, S., Nihal, M. & Ahmad, N. Dose translation from animal to human studies revisited. FASEB J. 22 (3), 659\u2013661 (2008).","journal-title":"FASEB J."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-60535-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-60535-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-60535-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:11:26Z","timestamp":1792451486000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-60535-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,19]]},"references-count":28,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["60535"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-60535-5","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,19]]},"assertion":[{"value":"24 October 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 June 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"32934"}}