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Here, we generated a 168\u00a0Mb genome assembly of\n                    <jats:italic>A<\/jats:italic>\n                    . s\n                    <jats:italic>implex<\/jats:italic>\n                    , the most abundant species in the Northeast Atlantic, which was used to develop SNP resources using a whole genome pooling resequencing strategy. 1,824,098 SNPs and 151,767 short structural variants were identified, showing the high polymorphism and complexity of\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>simplex<\/jats:italic>\n                    genome. A 481 SNP panel was developed for population screening using the Agriseq technology, through strict filtering and homogeneous distribution across the genome. This panel was used for genotyping 823 larvae and adults of\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>simplex<\/jats:italic>\n                    , 775\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>pegreffii<\/jats:italic>\n                    and 402 putative hybrids collected from different hosts across the Northeast Atlantic, 275 SNPs being consistently genotyped in both species. Among them, 10 SNPs showing extreme genetic differentiation between\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>simplex<\/jats:italic>\n                    and\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>pegreffii<\/jats:italic>\n                    and no linkage disequilibrium in\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>simplex<\/jats:italic>\n                    , were used to design a cost-effective SNP tool for species and hybrid identification in population surveys. The genomic resources obtained will facilitate efficient surveillance programs, accurate discrimination of species and hybrids, effective anisakiasis management in fisheries, and improved seafood safety.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-60679-4","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T14:44:54Z","timestamp":1783781094000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome assembly and SNP resources of Anisakis simplex enable cost-effective population assessment and discrimination of species and hybrids"],"prefix":"10.1038","volume":"16","author":[{"given":"Paulino","family":"Mart\u00ednez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Casanova-Chiclana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Urdaniz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Blanco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ramilo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"R\u00edos-Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"G\u00f3mez-Garrido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Gut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Alioto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Abollo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Pascual","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,11]]},"reference":[{"key":"60679_CR1","doi-asserted-by":"publisher","first-page":"13664","DOI":"10.1038\/s41598-022-17710-1","volume":"12","author":"P Cipriani","year":"2022","unstructured":"Cipriani, P. et al. 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