{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T07:30:33Z","timestamp":1792481433251,"version":"4.5.0"},"reference-count":0,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006673","name":"Fonds L\u00e9on Fredericq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006673","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["J.0069.22 and T.0095.24."],"award-info":[{"award-number":["J.0069.22 and T.0095.24."]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Kidney transplantation from donation after circulatory death (DCD) donors is increasingly used but remains associated with warm ischemic injury. Normothermic machine perfusion (NMP) enables functional assessment and therapeutic interventions. Mesenchymal stromal cells (MSCs) display immunomodulatory and regenerative properties, yet their translational efficacy during NMP remains unclear. In a porcine model, six pairs of kidneys subjected to 30\u00a0min of warm ischemia followed by 3\u00a0h of static cold storage underwent 6\u00a0h of NMP. In each pair, one kidney randomly received an intra-arterial injection of placebo, while the contralateral kidney received 10\u00a0million clinical-grade human bone marrow\u2013derived MSCs (hMSCs). Perfusion characteristics, glomerular filtration, tissue injury, and inflammatory markers were assessed. hMSC infusion during NMP was technically feasible and hemodynamically well tolerated, with no adverse effects on perfusion stability. Perfusion parameters, urine output, and creatinine\/iohexol clearance showed no significant differences between groups. NGAL and cytokines levels increased during perfusion, but hMSCs did not alter their dynamics. Intra-arterial delivery of clinical grade hMSCs during NMP was safe but did not improve renal function or reduce histological injury. These results highlight the challenges of achieving MSC engraftment during\n                    <jats:italic>ex situ<\/jats:italic>\n                    perfusion and highlight the need for refined strategies such as repeated dosing, prolonged perfusion, or extracellular vesicle therapy. Despite the negative findings, this study presents a highly translational large-animal model, supporting further investigation of MSC-based therapies in human kidneys discarded for transplantation.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-61509-3","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T02:34:23Z","timestamp":1783650863000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Infusion of human mesenchymal stromal cells during normothermic machine perfusion of porcine kidneys: a randomized, blinded, preclinical study"],"prefix":"10.1038","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1029-6269","authenticated-orcid":false,"given":"Margaux","family":"Navez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Gilbo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgan","family":"Vandermeulen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Erpicum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc Gilbert","family":"Lagny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarisse","family":"Malisoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Guarin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago Pinto","family":"Coelho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Thierry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Lechanteur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Briquet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Baudoux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Le Goff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Cavalier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo","family":"Caers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Jouret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Detry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,10]]},"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-61509-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T02:34:24Z","timestamp":1783650864000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-61509-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,10]]},"references-count":0,"alternative-id":["61509"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-61509-3","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,10]]},"assertion":[{"value":"12 May 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"This study was reviewed and approved by the Animal Ethics Committee of the University of Liege (approval number 21-2447) under the approved project entitled \u201cEffects of normothermic perfusion of porcine kidneys with porcine mesenchymal stromal cells (MSCs) on ischemia\u2013reperfusion injury\u201d, approved on March 28, 2022. Animal care and handling complied with the guidelines set out in the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health and the National Academy of Sciences, ensuring ethical and humane treatment throughout the experimental process. Human mesenchymal stromal cells were obtained from donors who provided written informed consent for the use of their biological samples for research purposes, in accordance with institutional and national regulations. The use of Human MSCs was reviewed and approved by the Hospital\u2013Faculty Ethics Committee of the University of Li\u00e8ge under the approved project entitled \u201cTB0202F11-Consent-collecte moelle allo MSC\u201d, with approval reference number 2025\u2009\u2212\u2009470, approved on November 7, 2024.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}