{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:14:50Z","timestamp":1788347690598,"version":"build-2803163510"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":34,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"the Joint Project on Regional High-Incidence Diseases Research of Guangxi Natural Science Foundation","award":["2024GXNSFAA010085"],"award-info":[{"award-number":["2024GXNSFAA010085"]}]},{"name":"the Joint Project on Regional High-Incidence Diseases Research of Guangxi Natural Science Foundation","award":["2024GXNSFAA010057"],"award-info":[{"award-number":["2024GXNSFAA010057"]}]},{"name":"the Advanced Innovation Teams and Xinghu Scholars Program of Guangxi Medical University","award":["2022"],"award-info":[{"award-number":["2022"]}]},{"name":"the China Undergraduate Innovation and Entrepreneurship Training Program","award":["202510598038"],"award-info":[{"award-number":["202510598038"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-026-62723-9","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T01:46:21Z","timestamp":1785375981000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated LiP-MS and quantitative proteomics reveal coordinated alterations in protein conformation and expression across tumor and peritumoral regions in hepatocellular carcinoma"],"prefix":"10.1038","volume":"16","author":[{"given":"Zhen-dong","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-ying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi-ni","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin-yun","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui-ling","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-wu","family":"Dang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan-dan","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"62723_CR1","doi-asserted-by":"publisher","first-page":"229","DOI":"10.3322\/caac.21834","volume":"74","author":"F Bray","year":"2024","unstructured":"Bray, F. et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 74, 229\u2013263. https:\/\/doi.org\/10.3322\/caac.21834 (2024).","journal-title":"CA Cancer J. Clin."},{"key":"62723_CR2","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1038\/s41572-020-00240-3","volume":"7","author":"JM Llovet","year":"2021","unstructured":"Llovet, J. M. et al. Hepatocellular carcinoma. Nat. Rev. Dis. Primers. 7, 6. https:\/\/doi.org\/10.1038\/s41572-020-00240-3 (2021).","journal-title":"Nat. Rev. Dis. Primers"},{"key":"62723_CR3","doi-asserted-by":"publisher","first-page":"1599","DOI":"10.1038\/s41591-022-01868-2","volume":"28","author":"AX Zhu","year":"2022","unstructured":"Zhu, A. X. et al. Molecular correlates of clinical response and resistance to atezolizumab in combination with bevacizumab in advanced hepatocellular carcinoma. Nat. Med. 28, 1599\u20131611. https:\/\/doi.org\/10.1038\/s41591-022-01868-2 (2022).","journal-title":"Nat. Med."},{"key":"62723_CR4","doi-asserted-by":"publisher","first-page":"2669","DOI":"10.1038\/s41467-022-30342-3","volume":"13","author":"Y Zhang","year":"2022","unstructured":"Zhang, Y., Chen, F., Chandrashekar, D. S., Varambally, S. & Creighton, C. J. Proteogenomic characterization of 2002 human cancers reveals pan-cancer molecular subtypes and associated pathways. Nat. Commun. 13, 2669. https:\/\/doi.org\/10.1038\/s41467-022-30342-3 (2022).","journal-title":"Nat. Commun."},{"key":"62723_CR5","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/j.cell.2019.08.052","volume":"179","author":"Q Gao","year":"2019","unstructured":"Gao, Q. et al. Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma. Cell 179, 561\u2013577e522. https:\/\/doi.org\/10.1016\/j.cell.2019.08.052 (2019).","journal-title":"Cell"},{"key":"62723_CR6","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1039\/d2an01246d","volume":"148","author":"RM Miller","year":"2023","unstructured":"Miller, R. M. & Smith, L. M. Overview and considerations in bottom-up proteomics. Analyst 148, 475\u2013486. https:\/\/doi.org\/10.1039\/d2an01246d (2023).","journal-title":"Analyst"},{"key":"62723_CR7","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/j.cell.2020.12.021","volume":"184","author":"V Cappelletti","year":"2021","unstructured":"Cappelletti, V. et al. Dynamic 3D proteomes reveal protein functional alterations at high resolution in situ. Cell 184, 545\u2013559e522. https:\/\/doi.org\/10.1016\/j.cell.2020.12.021 (2021).","journal-title":"Cell"},{"key":"62723_CR8","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1039\/d3cb00114h","volume":"4","author":"R Nussinov","year":"2023","unstructured":"Nussinov, R., Liu, Y., Zhang, W. & Jang, H. Protein conformational ensembles in function: roles and mechanisms. RSC Chem. Biol. 4, 850\u2013864. https:\/\/doi.org\/10.1039\/d3cb00114h (2023).","journal-title":"RSC Chem. Biol."},{"key":"62723_CR9","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1038\/s41596-022-00771-x","volume":"18","author":"L Malinovska","year":"2023","unstructured":"Malinovska, L. et al. Proteome-wide structural changes measured with limited proteolysis-mass spectrometry: an advanced protocol for high-throughput applications. Nat. Protoc. 18, 659\u2013682. https:\/\/doi.org\/10.1038\/s41596-022-00771-x (2023).","journal-title":"Nat. Protoc."},{"key":"62723_CR10","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/j.tibs.2024.11.005","volume":"50","author":"C Van Leene","year":"2025","unstructured":"Van Leene, C., Van Moortel, L., De Bosscher, K. & Gevaert, K. Exploring protein conformations with limited proteolysis coupled to mass spectrometry. Trends Biochem. Sci. 50, 143\u2013155. https:\/\/doi.org\/10.1016\/j.tibs.2024.11.005 (2025).","journal-title":"Trends Biochem. Sci."},{"key":"62723_CR11","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21908","author":"H Lu","year":"2024","unstructured":"Lu, H., Zhu, Z., Fields, L., Zhang, H. & Li, L. Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross-Linking. Mass Spectrom. Rev. https:\/\/doi.org\/10.1002\/mas.21908 (2024).","journal-title":"Mass Spectrom. Rev."},{"key":"62723_CR12","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1038\/nmeth.1322","volume":"6","author":"JR Wi\u015bniewski","year":"2009","unstructured":"Wi\u015bniewski, J. R., Zougman, A., Nagaraj, N. & Mann, M. Universal sample preparation method for proteome analysis. Nat. Methods. 6, 359\u2013362. https:\/\/doi.org\/10.1038\/nmeth.1322 (2009).","journal-title":"Nat. Methods"},{"key":"62723_CR13","doi-asserted-by":"publisher","first-page":"3420","DOI":"10.1093\/nar\/gkn176","volume":"36","author":"S G\u00f6tz","year":"2008","unstructured":"G\u00f6tz, S. et al. High-throughput functional annotation and data mining with the Blast2GO suite. Nucleic Acids Res. 36, 3420\u20133435. https:\/\/doi.org\/10.1093\/nar\/gkn176 (2008).","journal-title":"Nucleic Acids Res."},{"key":"62723_CR14","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1016\/j.jmb.2015.11.006","volume":"428","author":"M Kanehisa","year":"2016","unstructured":"Kanehisa, M., Sato, Y. & Morishima, K. BlastKOALA and GhostKOALA: KEGG Tools for Functional Characterization of Genome and Metagenome Sequences. J. Mol. Biol. 428, 726\u2013731. https:\/\/doi.org\/10.1016\/j.jmb.2015.11.006 (2016).","journal-title":"J. Mol. Biol."},{"key":"62723_CR15","doi-asserted-by":"publisher","first-page":"D344","DOI":"10.1093\/nar\/gkaa977","volume":"49","author":"M Blum","year":"2021","unstructured":"Blum, M. et al. The InterPro protein families and domains database: 20 years on. Nucleic Acids Res. 49, D344\u2013d354. https:\/\/doi.org\/10.1093\/nar\/gkaa977 (2021).","journal-title":"Nucleic Acids Res."},{"key":"62723_CR16","doi-asserted-by":"publisher","first-page":"W585","DOI":"10.1093\/nar\/gkm259","volume":"35","author":"P Horton","year":"2007","unstructured":"Horton, P. et al. WoLF PSORT: protein localization predictor. Nucleic Acids Res. 35, W585\u2013587. https:\/\/doi.org\/10.1093\/nar\/gkm259 (2007).","journal-title":"Nucleic Acids Res."},{"key":"62723_CR17","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1038\/s41422-023-00831-1","volume":"33","author":"L Wu","year":"2023","unstructured":"Wu, L. et al. An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression. Cell. Res. 33, 585\u2013603. https:\/\/doi.org\/10.1038\/s41422-023-00831-1 (2023).","journal-title":"Cell. Res."},{"key":"62723_CR18","doi-asserted-by":"publisher","first-page":"111731","DOI":"10.1016\/j.isci.2024.111731","volume":"28","author":"S Chang","year":"2025","unstructured":"Chang, S. et al. Succinate supplementation alleviates liver cancer by inhibiting the FN1\/SQLE axis-mediated cholesterol biosynthesis. iScience 28, 111731. https:\/\/doi.org\/10.1016\/j.isci.2024.111731 (2025).","journal-title":"iScience"},{"key":"62723_CR19","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1021\/acs.biochem.5c00009","volume":"64","author":"S Upadhyay","year":"2025","unstructured":"Upadhyay, S. et al. Impact of Cancer-Associated PKM2 Mutations on Enzyme Activity and Allosteric Regulation: Structural and Functional Insights into Metabolic Reprogramming. Biochemistry 64, 1463\u20131475. https:\/\/doi.org\/10.1021\/acs.biochem.5c00009 (2025).","journal-title":"Biochemistry"},{"key":"62723_CR20","doi-asserted-by":"publisher","first-page":"1164","DOI":"10.1097\/hep.0000000000000962","volume":"81","author":"Q Liu","year":"2025","unstructured":"Liu, Q. et al. Comprehensive profiling of lipid metabolic reprogramming expands precision medicine for HCC. Hepatology 81, 1164\u20131180. https:\/\/doi.org\/10.1097\/hep.0000000000000962 (2025).","journal-title":"Hepatology"},{"key":"62723_CR21","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1038\/s12276-025-01415-2","volume":"57","author":"S Park","year":"2025","unstructured":"Park, S. & Hall, M. N. Metabolic reprogramming in hepatocellular carcinoma: mechanisms and therapeutic implications. Exp. Mol. Med. 57, 515\u2013523. https:\/\/doi.org\/10.1038\/s12276-025-01415-2 (2025).","journal-title":"Exp. Mol. Med."},{"key":"62723_CR22","doi-asserted-by":"publisher","first-page":"1562","DOI":"10.1038\/s41587-024-02432-8","volume":"43","author":"C D\u00f6rig","year":"2025","unstructured":"D\u00f6rig, C. et al. Global profiling of protein complex dynamics with an experimental library of protein interaction markers. Nat. Biotechnol. 43, 1562\u20131576. https:\/\/doi.org\/10.1038\/s41587-024-02432-8 (2025).","journal-title":"Nat. Biotechnol."},{"key":"62723_CR23","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1038\/s41594-023-01203-4","volume":"31","author":"J D\u00fcring","year":"2024","unstructured":"D\u00fcring, J. et al. Structural mechanisms of autoinhibition and substrate recognition by the ubiquitin ligase HACE1. Nat. Struct. Mol. Biol. 31, 364\u2013377. https:\/\/doi.org\/10.1038\/s41594-023-01203-4 (2024).","journal-title":"Nat. Struct. Mol. Biol."},{"key":"62723_CR24","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1126\/science.add7859","volume":"381","author":"P Juyoux","year":"2023","unstructured":"Juyoux, P. et al. Architecture of the MKK6-p38\u03b1 complex defines the basis of MAPK specificity and activation. Science 381, 1217\u20131225. https:\/\/doi.org\/10.1126\/science.add7859 (2023).","journal-title":"Science"},{"key":"62723_CR25","doi-asserted-by":"publisher","first-page":"8627","DOI":"10.1038\/s41467-024-52995-y","volume":"15","author":"S Riedl","year":"2024","unstructured":"Riedl, S. et al. Evolution of the conformational dynamics of the molecular chaperone Hsp90. Nat. Commun. 15, 8627. https:\/\/doi.org\/10.1038\/s41467-024-52995-y (2024).","journal-title":"Nat. Commun."},{"key":"62723_CR26","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1186\/s40364-022-00396-y","volume":"10","author":"H Meng","year":"2022","unstructured":"Meng, H. et al. ZG16 promotes T-cell mediated immunity through direct binding to PD-L1 in colon cancer. Biomark. Res. 10, 47. https:\/\/doi.org\/10.1186\/s40364-022-00396-y (2022).","journal-title":"Biomark. Res."},{"key":"62723_CR27","doi-asserted-by":"publisher","first-page":"10100","DOI":"10.1038\/s41467-024-54438-0","volume":"15","author":"Y Xu","year":"2024","unstructured":"Xu, Y. et al. Multimodal single cell-resolved spatial proteomics reveal pancreatic tumor heterogeneity. Nat. Commun. 15, 10100. https:\/\/doi.org\/10.1038\/s41467-024-54438-0 (2024).","journal-title":"Nat. Commun."},{"key":"62723_CR28","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1074\/mcp.RA117.000189","volume":"17","author":"K Buczak","year":"2018","unstructured":"Buczak, K. et al. Spatial Tissue Proteomics Quantifies Inter- and Intratumor Heterogeneity in Hepatocellular Carcinoma (HCC). Mol. Cell. Proteom. 17, 810\u2013825. https:\/\/doi.org\/10.1074\/mcp.RA117.000189 (2018).","journal-title":"Mol. Cell. Proteom."},{"key":"62723_CR29","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1186\/s12935-023-03027-0","volume":"23","author":"B Ma","year":"2023","unstructured":"Ma, B. et al. Spatially resolved visualization of reprogrammed metabolism in hepatocellular carcinoma by mass spectrometry imaging. Cancer Cell. Int. 23, 177. https:\/\/doi.org\/10.1186\/s12935-023-03027-0 (2023).","journal-title":"Cancer Cell. Int."},{"key":"62723_CR30","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.canlet.2019.12.010","volume":"471","author":"P Icard","year":"2020","unstructured":"Icard, P. et al. ATP citrate lyase: A central metabolic enzyme in cancer. Cancer Lett. 471, 125\u2013134. https:\/\/doi.org\/10.1016\/j.canlet.2019.12.010 (2020).","journal-title":"Cancer Lett."},{"key":"62723_CR31","doi-asserted-by":"publisher","first-page":"eadf0115","DOI":"10.1126\/sciadv.adf0115","volume":"9","author":"LT Izzo","year":"2023","unstructured":"Izzo, L. T. et al. Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. Sci. Adv. 9, eadf0115. https:\/\/doi.org\/10.1126\/sciadv.adf0115 (2023).","journal-title":"Sci. Adv."},{"key":"62723_CR32","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1016\/j.jhep.2019.10.026","volume":"72","author":"Z Ding","year":"2020","unstructured":"Ding, Z. et al. Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis. J. Hepatol. 72, 725\u2013735. https:\/\/doi.org\/10.1016\/j.jhep.2019.10.026 (2020).","journal-title":"J. Hepatol."},{"key":"62723_CR33","doi-asserted-by":"publisher","first-page":"1769","DOI":"10.1007\/s00726-021-03060-1","volume":"53","author":"P Geng","year":"2021","unstructured":"Geng, P., Qin, W. & Xu, G. Proline metabolism in cancer. Amino Acids. 53, 1769\u20131777. https:\/\/doi.org\/10.1007\/s00726-021-03060-1 (2021).","journal-title":"Amino Acids"},{"key":"62723_CR34","doi-asserted-by":"publisher","first-page":"776","DOI":"10.3389\/fonc.2020.00776","volume":"10","author":"C D\u2019Aniello","year":"2020","unstructured":"D\u2019Aniello, C., Patriarca, E. J., Phang, J. M. & Minchiotti, G. Proline Metabolism in Tumor Growth and Metastatic Progression. Front. Oncol. 10, 776. https:\/\/doi.org\/10.3389\/fonc.2020.00776 (2020).","journal-title":"Front. Oncol."},{"key":"62723_CR35","doi-asserted-by":"publisher","first-page":"1807","DOI":"10.1007\/s00726-021-02961-5","volume":"53","author":"Z Ding","year":"2021","unstructured":"Ding, Z., Ericksen, R. E., Lee, Q. Y. & Han, W. Reprogramming of mitochondrial proline metabolism promotes liver tumorigenesis. Amino Acids. 53, 1807\u20131815. https:\/\/doi.org\/10.1007\/s00726-021-02961-5 (2021).","journal-title":"Amino Acids"},{"key":"62723_CR36","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1002\/path.5665","volume":"254","author":"Q Wang","year":"2021","unstructured":"Wang, Q. et al. Inhibition of guanosine monophosphate synthetase (GMPS) blocks glutamine metabolism and prostate cancer growth. J. Pathol. 254, 135\u2013146. https:\/\/doi.org\/10.1002\/path.5665 (2021).","journal-title":"J. Pathol."},{"key":"62723_CR37","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1186\/s13046-022-02384-4","volume":"41","author":"L Chellini","year":"2022","unstructured":"Chellini, L., Pieraccioli, M., Sette, C. & Paronetto, M. P. The DNA\/RNA helicase DHX9 contributes to the transcriptional program of the androgen receptor in prostate cancer. J. Exp. Clin. Cancer Res. 41, 178. https:\/\/doi.org\/10.1186\/s13046-022-02384-4 (2022).","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"62723_CR38","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1038\/s41388-023-02894-1","volume":"43","author":"L Chellini","year":"2024","unstructured":"Chellini, L., Scarf\u00f2, M., Bonvissuto, D., Sette, C. & Paronetto, M. P. The DNA\/RNA helicase DHX9 orchestrates the KDM2B-mediated transcriptional regulation of YAP1 in Ewing sarcoma. Oncogene 43, 225\u2013234. https:\/\/doi.org\/10.1038\/s41388-023-02894-1 (2024).","journal-title":"Oncogene"},{"key":"62723_CR39","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1038\/s41586-019-0987-8","volume":"567","author":"Y Jiang","year":"2019","unstructured":"Jiang, Y. et al. Proteomics identifies new therapeutic targets of early-stage hepatocellular carcinoma. Nature 567, 257\u2013261. https:\/\/doi.org\/10.1038\/s41586-019-0987-8 (2019).","journal-title":"Nature"},{"key":"62723_CR40","doi-asserted-by":"publisher","first-page":"D1211","DOI":"10.1093\/nar\/gky869","volume":"47","author":"J Ma","year":"2019","unstructured":"Ma, J. et al. iProX: an integrated proteome resource. Nucleic Acids Res. 47, D1211\u2013d1217. https:\/\/doi.org\/10.1093\/nar\/gky869 (2019).","journal-title":"Nucleic Acids Res."},{"key":"62723_CR41","doi-asserted-by":"publisher","first-page":"D1522","DOI":"10.1093\/nar\/gkab1081","volume":"50","author":"T Chen","year":"2022","unstructured":"Chen, T. et al. iProX in 2021: connecting proteomics data sharing with big data. Nucleic Acids Res. 50, D1522\u2013d1527. https:\/\/doi.org\/10.1093\/nar\/gkab1081 (2022).","journal-title":"Nucleic Acids Res."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-62723-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-62723-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-62723-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:31:04Z","timestamp":1788345064000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-026-62723-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["62723"],"URL":"https:\/\/doi.org\/10.1038\/s41598-026-62723-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]},"assertion":[{"value":"4 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was approved by the Ethics Committee of the First Affiliated Hospital of Guangxi Medical University (No. 2025-S0002), and written informed consent was obtained from all participants prior to sample collection. All methods were performed in accordance with the relevant guidelines and regulations.","order":2,"name":"Ethics","label":"Ethics approval and consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"27520"}}