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This study aimed to isolate an EPS-producing bacterium from naturally fermented cucumber and to perform a preliminary physicochemical and spectroscopic characterization of the produced polymer. An EPS-producing bacterial isolate was recovered and identified as\n                    <jats:italic>Bacillus tequilensis<\/jats:italic>\n                    , a member of the\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    species complex, based on 16\u00a0S rRNA gene sequence analysis. The extracellular polysaccharide was partially purified and analyzed using FTIR and \u00b9H NMR spectroscopy, revealing structural features consistent with a glucan-type EPS. Rheological analysis demonstrated a concentration-dependent increase in viscosity, confirming the macromolecular nature of the polymer in aqueous systems. Sucrose-hydrolyzing activity was detected under optimized conditions, indicating active carbohydrate metabolism associated with EPS production. In vitro cytocompatibility assessment using Vero and WI-38 cell lines showed high cell viability at low to moderate concentrations, with a dose-dependent reduction observed at higher levels. Overall, this study reports the isolation of an EPS-producing\n                    <jats:italic>Bacillus tequilensis<\/jats:italic>\n                    strain from Egyptian fermented cucumber and provides preliminary insights into the properties of its glucan-type exopolysaccharide, highlighting fermented vegetable microbiota as a potential source of functional biopolymers.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-62841-4","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:05:45Z","timestamp":1785434745000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation and preliminary characterization of a glucan-type exopolysaccharide produced by Bacillus tequilensis strain HH from Egyptian fermented cucumber"],"prefix":"10.1038","volume":"16","author":[{"given":"H. 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