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Here, we used IPT to compare the fate of dissociated E12.5 mouse pancreatic bud-derived cells in non-knockout recipients retaining an endogenous pancreatic developmental field and\n                    <jats:italic>Pdx1<\/jats:italic>\n                    -deficient mouse recipients. In a subset of non-knockout hosts, donor-derived cells were detected within endocrine, acinar, and ductal compartments, indicating donor-cell localization within pancreatic tissue compartments after transplantation. In contrast, grossly examined\n                    <jats:italic>Pdx1<\/jats:italic>\n                    -deficient recipients did not form macroscopically detectable organized pancreatic tissue. Some KO recipients in a serum-insulin endpoint cohort showed detectable circulating insulin. In a separate survival\/blood-glucose cohort, IPT KO recipients showed transient postnatal increases in blood glucose followed by progressive hypoglycemia; survival and overall physiology were not normalized. Donor-cell localization in\n                    <jats:italic>Pdx1<\/jats:italic>\n                    -deficient recipients was not determined in this study. Thus, under the present donor-preparation and IPT conditions, E12.5 pancreatic bud-derived cells were detected within pancreatic tissue compartments in a subset of non-KO recipients but did not form macroscopically detectable organized pancreatic tissue in\n                    <jats:italic>Pdx1<\/jats:italic>\n                    -deficient recipients. Separately, analysis of published single-cell RNA-sequencing datasets, together with flow-cytometric validation, supported EPCAM as a reporter-independent epithelial enrichment marker that captures a large fraction of\n                    <jats:italic>Pdx1<\/jats:italic>\n                    -expressing epithelial cells in the E12.5 pancreatic bud. These parallel findings show that transplantation outcome differed between non-KO recipients retaining an endogenous pancreatic field and\n                    <jats:italic>Pdx1<\/jats:italic>\n                    -deficient recipients under the present IPT conditions and also support a practical strategy for enriching embryonic pancreatic epithelial fractions containing\n                    <jats:italic>Pdx1<\/jats:italic>\n                    -expressing cells.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-65314-w","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:03:34Z","timestamp":1789376614000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["E12.5 pancreatic bud-derived cells are detected in recipient pancreata after intraplacental transplantation, but Pdx1-deficient recipients lack a macroscopically detectable pancreas"],"prefix":"10.1038","volume":"16","author":[{"given":"Zeynab","family":"Javanfekr Shahri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Noda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuto","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Wei","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Gogoleva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arata","family":"Wakimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Kumaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michito","family":"Hamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"65314_CR1","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1016\/j.cell.2014.09.040","volume":"159","author":"FW Pagliuca","year":"2014","unstructured":"Pagliuca, F. 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