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Eggs and egg products represent one of the most common sources of human salmonellosis, primarily caused by\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    serovar\n                    <jats:italic>Enteritidis<\/jats:italic>\n                    (\n                    <jats:italic>S. enteritidis<\/jats:italic>\n                    ). Accordingly, the present study was conducted to perform a molecular investigation of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    and\n                    <jats:italic>S. enteritidis<\/jats:italic>\n                    in table eggs and stool samples obtained from diarrheic patients. A total of 58 pooled samples of fresh hen\u2019s eggs, along with 34 stool samples, were collected and subjected to DNA extraction followed by polymerase chain reaction (PCR) analysis. The results revealed the presence of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    and\n                    <jats:italic>S. enteritidis<\/jats:italic>\n                    DNA in 55.2% and 50% of the examined egg samples, respectively. A higher prevalence of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    was detected on eggshells compared to egg contents. Variations in infection rates were observed based on gender and age;\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    infections were more frequently identified in males and younger individuals, whereas\n                    <jats:italic>S. enteritidis<\/jats:italic>\n                    infections were more common among females and older children. Gastrointestinal symptoms were associated with both infections, with abdominal pain being particularly prevalent in patients infected with\n                    <jats:italic>S. enteritidis<\/jats:italic>\n                    . In conclusion, the findings of this study highlight the potential zoonotic risk posed to consumers and underscore the urgent need for implementing effective control programs in laying hen farms.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-66486-1","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T09:25:04Z","timestamp":1787217904000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular investigation on Listeria monocytogenes and Salmonella enteritidis in table eggs and diarrheic patients"],"prefix":"10.1038","volume":"16","author":[{"given":"Asmaa A.","family":"Rayan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reem M.","family":"Alsaadawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa G.","family":"Abd El Kader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"66486_CR1","first-page":"249","volume":"4","author":"H Ghasemian Safaei","year":"2011","unstructured":"Ghasemian Safaei, H., Hosseini, J. 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