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The methods integrate first-derivative spectrophotometry (D\n                    <jats:sup>1<\/jats:sup>\n                    ) and Fourier Self-Deconvolution (FSD) to resolve the extensive spectral overlap between GLI and its alkaline degradation product (Gliclazide Impurity A) without prior separation. The assay demonstrated excellent linearity within 2.5\u201325\u00a0\u00b5g\/mL for GLI and 2.5\u201315\u00a0\u00b5g\/mL for its degradation product. LOD ranged from 0.31 to 0.43\u00a0\u00b5g\/mL and LOQ from 0.94 to 1.31\u00a0\u00b5g\/mL for both analytes. Using ethanol as a green, non-toxic solvent further enhanced the method\u2019s environmental sustainability. Method validation, performed according to ICH Q2(R1) guidelines, confirmed its selectivity, reproducibility, robustness, and accuracy with mean recoveries of 99.48\u2009\u00b1\u20090.83 and 100.87\u2009\u00b1\u20091.52 for GLI in the D\n                    <jats:sup>1<\/jats:sup>\n                    and FSD methods, respectively. Comprehensive forced degradation studies under alkaline stress verified the procedure\u2019s stability-indicating capability, enabling reliable quantification of GLI in the presence of its degradation products. The greenness evaluation using Analytical Eco-Scale, GAPI, AGREE, NEMI tools, EPPI tool, AGSA tool, and MOGAPI method collectively affirmed the method\u2019s eco-friendly and sustainable nature. Furthermore, the analytical strategy was comprehensively assessed using multi-dimensional tools, including RAPI and AMRI for analytical performance and reliability, CaFRI for carbon-footprint reduction, CACI for analytical practicality, and the RGB 12 model for an integrated multi-colored analytical profile. In summary, the developed methods provide a highly efficient, environmentally friendly, and analytical alternative to conventional chromatographic techniques for the routine quality control and green pharmaceutical analysis of gliclazide in bulk and dosage forms.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-67045-4","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T17:05:26Z","timestamp":1787418326000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sustainable stability-indicating spectra manipulations for the concurrent quantification of gliclazide and its impurity A as the possible alkaline degradation product: Multi-Color Analytical Profile Assessment"],"prefix":"10.1038","volume":"16","author":[{"given":"Ebraam B.","family":"Kamel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed Elsayed","family":"Abdelmegeed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanaa","family":"Saleh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Elhenawee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman A.","family":"Bahgat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,22]]},"reference":[{"key":"67045_CR1","doi-asserted-by":"publisher","first-page":"1687","DOI":"10.1007\/s13300-024-01612-8","volume":"15","author":"I Sahin","year":"2024","unstructured":"Sahin, I., Bakiner, O., Demir, T., Sari, R. & Atmaca, A. 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