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This study aimed to isolate, phenotypically characterize, and perform whole-genome sequencing of the bacteriophage demonstrating the broadest host range against MDR uropathogens. Fifty MDR GNB isolates were screened for lytic phages. The most promising candidate,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    phage KP \u00d81, was characterized using plaque assay, Transmission Electron Microscopy (TEM), and pH\/thermal stability testing. Genomic characterization was performed via whole-genome sequencing (WGS), with functional annotation and lifestyle prediction using PhaBOX and PhageScope software.\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    was the most prevalent MDR uropathogen.\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    phage KP \u00d81 exhibited a 50% host range and high lytic titer (10\u2078 PFU\/mL). TEM revealed an icosahedral head and short contractile tail. Genomic characterization by WGS revealed that\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    phage KP \u00d81 possesses a 174,591\u00a0bp double-stranded deoxyribonucleic acid (dsDNA) genome containing 274 predicted open reading frames (ORFs). No lysogeny-related genes, toxins, or antibiotic resistance markers were detected, confirming its strictly lytic nature and supporting its potential as a candidate for phage therapy applications. The phage remained stable (10\u2078 PFU\/mL) across temperatures of \u2212\u200920\u00a0\u00b0C to 50\u00a0\u00b0C; supporting its suitability for long-term biobanking and suggesting potential activity at physiological temperature, and across a pH range of 7\u20139.\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    phage KP \u00d81 is a novel, obligately lytic\n                    <jats:italic>Slopekvirus<\/jats:italic>\n                    whose genomic architecture, stability profile, and absence of lysogeny-associated, virulence, and antimicrobial resistance genes ( AMR) collectively support its candidacy for further preclinical evaluation as a phage therapy agent against uropathogenic MDR\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-67695-4","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T07:12:35Z","timestamp":1788505955000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and phenotypic characterization of Klebsiella pneumoniae phage KP \u00d81: a novel lytic Slopekvirus targeting uropathogenic multidrug-resistant Klebsiella pneumoniae"],"prefix":"10.1038","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5368-0837","authenticated-orcid":false,"given":"Reham","family":"Fadl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghada","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6140-6379","authenticated-orcid":false,"given":"Ahmed","family":"Elshafei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira Mohamed","family":"Mokhtar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omnia Mohamed","family":"El Nabawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Gomaa Seadawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud M.","family":"Baraka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basma","family":"Sherif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"issue":"2","key":"67695_CR1","doi-asserted-by":"publisher","first-page":"59","DOI":"10.3390\/diseases13020059","volume":"13","author":"B Baimakhanova","year":"2025","unstructured":"Baimakhanova, B. et al. 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