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This study provides a comparative assessment of the larvicidal activity and phytochemical composition of n-hexane extracts from four Solanaceous plants:\n                    <jats:italic>Capsicum annuum<\/jats:italic>\n                    (Cap.),\n                    <jats:italic>Datura stramonium<\/jats:italic>\n                    (Dat.),\n                    <jats:italic>Nicotiana tabacum<\/jats:italic>\n                    (To.), and\n                    <jats:italic>Withania somnifera<\/jats:italic>\n                    (Wt.). Volatile constituents were characterized using GC\u2013MS analysis, and larvicidal activity was evaluated against\n                    <jats:italic>Culex pipiens<\/jats:italic>\n                    and\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    , with LC\u2085\u2080 and LC\u2089\u2080 values determined at 24 and 48\u00a0h. post-treatment. All extracts showed concentration- and time-dependent toxicity, achieving near-complete mortality at 1000 ppm for\n                    <jats:italic>N. tabacum<\/jats:italic>\n                    and\n                    <jats:italic>D. stramonium<\/jats:italic>\n                    .\n                    <jats:italic>Nicotiana<\/jats:italic>\n                    tabacum showed the highest efficacy against\n                    <jats:italic>Cx. pipiens<\/jats:italic>\n                    (LC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u2009120.45 and 95.30 ppm, at 24 and 48\u00a0h), whereas\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    showed comparatively lower susceptibility. Biochemical analyses revealed significant inhibition of acetylcholinesterase (AChE),\n                    <jats:italic>\u03b1<\/jats:italic>\n                    - and\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -esterases, and GABA-transaminase, alongside an imbalance in antioxidant defenses, suggesting a multi-system physiological impact. GC\u2013MS profiling identified distinct phytochemical compositions, particularly the alkaloid and fatty acid content identified in the more potent extracts. The four extracts contained a vast array of phytochemical components, reaching 110 components belonging to capsaicinoids and hydrocarbons for Cap; To. and Dat. were rich in fatty acids, particularly\n                    <jats:italic>n<\/jats:italic>\n                    -hexadecanoic and oleic acids; and Wt. exhibited a hydrocarbon-rich profile with cyclic and branched alkanes. Oleic acid was the only compound shared among three samples (Cap., To., and Dat.), whereas no compound was common across all samples. This study provides the first comparative assessment of four Solanaceous genera using integrated phytochemical, biochemical, and larvicidal analyses. The findings identify\n                    <jats:italic>N. tabacum<\/jats:italic>\n                    as the most promising candidate and establish a foundation for bioassay-guided development of plant-based bio-insecticides. This integrative approach offers a practical framework for screening botanical extracts for vector control programs, though further studies are needed to confirm the causal phytochemicals and mechanisms.\n                  <\/jats:p>","DOI":"10.1038\/s41598-026-68059-8","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:32:52Z","timestamp":1788611572000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative larvicidal efficacy and phytochemical profiling of selected Solanaceous hexane extracts against Culex pipiens and Aedes aegypti"],"prefix":"10.1038","volume":"16","author":[{"given":"Shaimaa H.","family":"Mohammed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esraa A.","family":"Elhawary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser A.","family":"El-Sayed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed M.","family":"Baz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelfattah M.","family":"Selim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed H.","family":"Alruhaili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hattan S.","family":"Gattan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randa I.","family":"Eltaly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,5]]},"reference":[{"key":"68059_CR1","first-page":"348","volume":"10","author":"A Al-Azab","year":"2022","unstructured":"Al-Azab, A., Zaituon, A., Al-Ghamdi, K. & Al-Galil, F. 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