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However, the impact of environmental temperature on mouse gastrointestinal physiology remains largely unexplored. Here we show that mice raised at 22\u2009\u00b0C exhibit whole gut transit speed nearly twice as fast as those raised at 30\u2009\u00b0C, primarily driven by a threefold increase in colon transit speed. Furthermore, gut microbiota composition differs between the two temperatures but does not dictate temperature-dependent differences in gut motility. Notably, increased stress signals from the hypothalamic\u2013pituitary\u2013adrenal axis at 22\u2009\u00b0C have a pivotal role in mediating temperature-dependent differences in gut motility. Pharmacological and genetic depletion of the stress hormone corticotropin-releasing hormone slows gut motility in stressed 22\u2009\u00b0C mice but has no comparable effect in relatively unstressed 30\u2009\u00b0C mice. In conclusion, our findings highlight that colder mouse facility temperatures significantly increase gut motility through hormonal stress pathways.<\/jats:p>","DOI":"10.1038\/s41684-024-01376-5","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T10:01:49Z","timestamp":1716890509000},"page":"148-159","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Temperature-dependent differences in mouse gut motility are mediated by stress"],"prefix":"10.1038","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4525-0866","authenticated-orcid":false,"given":"Alvin","family":"Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Courtney","family":"Hudson-Paz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz G.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laren","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Jacobson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2893-1793","authenticated-orcid":false,"given":"Julia A.","family":"Kaltschmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Garrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8099-2975","authenticated-orcid":false,"given":"Ami S.","family":"Bhatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3804-2209","authenticated-orcid":false,"given":"Denise M.","family":"Monack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,28]]},"reference":[{"key":"1376_CR1","unstructured":"ACD Working Group on Enhancing Rigor, Transparency, and Translatability in Animal Research 20 (National Institutes of Health, 2021)."},{"key":"1376_CR2","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1084\/jem.20120988","volume":"209","author":"CL Karp","year":"2012","unstructured":"Karp, C. 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