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This step is essential to enable iron uptake by the divalent metal transporter. Here we report the crystallographic structures of human Dcytb and its complex with ascorbate and Zn\n                    <jats:sup>2+<\/jats:sup>\n                    . Each monomer of the homodimeric protein possesses cytoplasmic and apical heme groups, as well as cytoplasmic and apical ascorbate-binding sites located adjacent to each heme. Zn\n                    <jats:sup>2+<\/jats:sup>\n                    coordinates to two hydroxyl groups of the apical ascorbate and to a histidine residue. Biochemical analysis indicates that Fe\n                    <jats:sup>3+<\/jats:sup>\n                    competes with Zn\n                    <jats:sup>2+<\/jats:sup>\n                    for this binding site. These results provide a structural basis for the mechanism by which Fe\n                    <jats:sup>3+<\/jats:sup>\n                    uptake is promoted by reducing agents and should facilitate structure-based development of improved agents for absorption of orally administered iron.\n                  <\/jats:p>","DOI":"10.1038\/s42003-018-0121-8","type":"journal-article","created":{"date-parts":[[2018,8,13]],"date-time":"2018-08-13T11:23:15Z","timestamp":1534159395000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate"],"prefix":"10.1038","volume":"1","author":[{"given":"Menega","family":"Ganasen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Togashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanae","family":"Takeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honami","family":"Asakura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8971-0759","authenticated-orcid":false,"given":"Takehiko","family":"Tosha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5442-7582","authenticated-orcid":false,"given":"Keitaro","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1491-6509","authenticated-orcid":false,"given":"Kunio","family":"Hirata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Nariai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Urano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iqbal","family":"Hamza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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