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In this study, we aimed to evaluate the roles of endogenous H<jats:sub>2<\/jats:sub>S in generating eupnea at the respiratory center. We employed an in situ arterially perfused preparation of decerebrated rats and recorded the central respiratory outputs. When the H<jats:sub>2<\/jats:sub>S-producing enzyme cystathionine \u03b2-synthase (CBS) was inhibited, respiration switched from the 3-phase eupneic pattern, which consists of inspiration, postinspiration, and expiration, to gasping-like respiration, which consists of inspiration only. On the other hand, when H<jats:sub>2<\/jats:sub>S synthesis was inhibited via cystathionine \u03b3-lyase (CSE) or when H<jats:sub>2<\/jats:sub>S synthesis was activated via CBS, eupnea remained unchanged. These results suggest that H<jats:sub>2<\/jats:sub>S produced by CBS has crucial roles in maintaining the neuronal network to generate eupnea. The mechanism of respiratory pattern generation might be switched from a network-based system to a pacemaker cell-based system in low H<jats:sub>2<\/jats:sub>S conditions.<\/jats:p>","DOI":"10.1038\/s42003-020-01312-6","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T10:02:55Z","timestamp":1602842575000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Endogenous hydrogen sulfide maintains eupnea in an in situ arterially perfused preparation of rats"],"prefix":"10.1038","volume":"3","author":[{"given":"Minako","family":"Okazaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saori","family":"Uozu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuma","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5962-6943","authenticated-orcid":false,"given":"Tadachika","family":"Koganezawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,10,16]]},"reference":[{"key":"1312_CR1","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1016\/j.neuint.2013.09.003","volume":"63","author":"H Kimura","year":"2013","unstructured":"Kimura, H. 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