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Here we show that <jats:italic>Toxoplasma gondii<\/jats:italic>\u2014a common intracellular pathogen of humans and animals\u2014can import and co-utilize <jats:italic>myo<\/jats:italic>-inositol with the endogenous CDP-diacylglycerol to synthesize PtdIns. Equally, the parasite harbors a functional PtdIns synthase (PIS) containing a catalytically-vital CDP-diacylglycerol phosphotransferase motif in the Golgi apparatus. Auxin-induced depletion of PIS abrogated the lytic cycle of <jats:italic>T. gondii<\/jats:italic> in human cells due to defects in cell division, gliding motility, invasion, and egress. Isotope labeling of the PIS mutant in conjunction with lipidomics demonstrated de novo synthesis of specific PtdIns species, while revealing the salvage of other lipid\u00a0species from the host cell. Not least, the mutant showed decline in phosphatidylthreonine, and elevation of selected phosphatidylserine and phosphatidylglycerol species, indicating a rerouting of CDP-diacylglycerol and homeostatic inter-regulation of anionic phospholipids upon knockdown of PIS.\u00a0In conclusion, strategic allocation of own and host-derived PtdIns species to gratify its metabolic demand features as\u00a0a notable adaptive trait of <jats:italic>T. gondii<\/jats:italic>. Conceivably, the dependence of <jats:italic>T. gondii<\/jats:italic> on de novo lipid synthesis and scavenging can be exploited to develop new anti-infectives.<\/jats:p>","DOI":"10.1038\/s42003-020-01480-5","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T11:09:12Z","timestamp":1607598552000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Phosphatidylinositol synthesis, its selective salvage, and inter-regulation of anionic phospholipids in Toxoplasma gondii"],"prefix":"10.1038","volume":"3","author":[{"given":"Bingjian","family":"Ren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Hedar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos F.","family":"Brouwers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1452-5126","authenticated-orcid":false,"given":"Nishith","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,12,10]]},"reference":[{"key":"1480_CR1","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1038\/nrmicro3139","volume":"11","author":"I Coppens","year":"2013","unstructured":"Coppens, I. 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