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One such effector, SseK3, is a Golgi-targeting arginine GlcNAc transferase. Here, we show that SseK3 colocalizes with\n                    <jats:italic>cis<\/jats:italic>\n                    -Golgi via lipid binding. Arg-GlcNAc-omics profiling reveals that SseK3 modifies Rab1 and some phylogenetically related Rab GTPases. These modifications are dependent on C-termini of Rabs but independent of the GTP- or GDP-bound forms. Arginine GlcNAcylation occurs in the switch II region and the third \u03b1-helix and severely disturbs the function of Rab1. The arginine GlcNAc transferase activity of SseK3 is required for the replication of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    in RAW264.7 macrophages and bacterial virulence in the mouse model of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    infection. Therefore, this SseK3 mechanism of action represents a new understanding of the strategy adopted by\n                    <jats:italic>Salmonella<\/jats:italic>\n                    to target host trafficking systems.\n                  <\/jats:p>","DOI":"10.1038\/s42003-020-1005-2","type":"journal-article","created":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T06:04:22Z","timestamp":1591337062000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium"],"prefix":"10.1038","volume":"3","author":[{"given":"Kun","family":"Meng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shufan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7083-5263","authenticated-orcid":false,"given":"Xiaoyun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9562-7791","authenticated-orcid":false,"given":"Feng","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8646-3193","authenticated-orcid":false,"given":"Shan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,5]]},"reference":[{"key":"1005_CR1","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1080\/21541248.2016.1192921","volume":"8","author":"S Srikanth","year":"2017","unstructured":"Srikanth, S., Woo, J. 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