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A subset of plant RLPs distinctively localizes to the plasma membrane-lined pores called plasmodesmata. Those RLPs include the <jats:italic>Arabidopsis thaliana<\/jats:italic> Plasmodesmata-located protein (PDLP) 5, which is well known for its vital function regulating plasmodesmal gating and molecular movement between cells. In this study, we report that the TMD, although not a determining factor for the plasmodesmal targeting, serves essential roles for the PDLP5 function. In addition to its role for membrane anchoring, the TMD mediates PDLP5 self-interaction and carries an evolutionarily conserved motif that is essential for PDLP5 to regulate cell-to-cell movement. Computational modeling-based analyses suggest that PDLP TMDs have high propensities to dimerize. We discuss how a specific mode(s) of TMD dimerization might serve as a common mechanism for PDLP5 and other PDLP members to regulate cell-to-cell movement.<\/jats:p>","DOI":"10.1038\/s42003-020-1007-0","type":"journal-article","created":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T10:04:22Z","timestamp":1591351462000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["An evolutionarily conserved motif is required for Plasmodesmata-located protein 5 to regulate cell-to-cell movement"],"prefix":"10.1038","volume":"3","author":[{"given":"Xu","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3409-5557","authenticated-orcid":false,"given":"Gabriel","family":"Robles Luna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0803-4817","authenticated-orcid":false,"given":"Cecilia Noemi","family":"Arighi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4604-7974","authenticated-orcid":false,"given":"Jung-Youn","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,5]]},"reference":[{"key":"1007_CR1","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1038\/s41477-018-0288-5","volume":"4","author":"L Liu","year":"2018","unstructured":"Liu, L. & Chen, X. 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