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STN dysregulation is correlated with several brain disorders, including Parkinson\u02bcs disease and obsessive compulsive disorder (OCD), for which high-frequency stimulation of the STN is increasing as therapy. However, clinical progress is hampered by poor knowledge of the anatomical\u2013functional organization of the STN. Today, experimental mouse genetics provides outstanding capacity for functional decoding, provided selective promoters are available. Here, we implemented single-nuclei RNA sequencing (snRNASeq) of the mouse STN followed through with histological analysis of 16 candidate genes of interest. Our results demonstrate that the mouse STN is composed of at least four spatio-molecularly defined domains, each distinguished by defined sets of promoter activities. Further, molecular profiles dissociate the STN from the adjoining para-STN (PSTN) and neighboring structures\u00a0of the hypothalamus, mammillary nuclei and zona incerta. Enhanced knowledge of STN\u00b4s internal organization should prove useful towards genetics-based functional decoding of this clinically relevant brain structure.<\/jats:p>","DOI":"10.1038\/s42003-020-1028-8","type":"journal-article","created":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T06:02:57Z","timestamp":1593756177000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Spatio-molecular domains identified in the mouse subthalamic nucleus and neighboring glutamatergic and GABAergic brain structures"],"prefix":"10.1038","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8713-070X","authenticated-orcid":false,"given":"\u00c5sa","family":"Wall\u00e9n-Mackenzie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4415-9924","authenticated-orcid":false,"given":"Sylvie","family":"Dumas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0845-9831","authenticated-orcid":false,"given":"Maria","family":"Papathanou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela M.","family":"Martis Thiele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5442-2303","authenticated-orcid":false,"given":"Bianca","family":"Vlcek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0899-046X","authenticated-orcid":false,"given":"Niclas","family":"K\u00f6nig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2224-7090","authenticated-orcid":false,"given":"\u00c5sa K.","family":"Bj\u00f6rklund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,3]]},"reference":[{"key":"1028_CR1","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1016\/j.pneurobio.2005.09.005","volume":"76","author":"Y Temel","year":"2005","unstructured":"Temel, Y., Blokland, A., Steinbusch, H. 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