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As a unique intracellular nematode,\n                    <jats:italic>Trichinella<\/jats:italic>\n                    consists of two clades, encapsulated and non-encapsulated. Genomic correlation of the distinct differences between the two clades is still unclear. Here, we report an annotated draft reference genome of non-encapsulated\n                    <jats:italic>Trichinella<\/jats:italic>\n                    ,\n                    <jats:italic>T. pseudospiralis<\/jats:italic>\n                    , and perform comparative multi-omics analyses with encapsulated\n                    <jats:italic>T. spiralis<\/jats:italic>\n                    . Genome and methylome analyses indicate that, during\n                    <jats:italic>Trichinella<\/jats:italic>\n                    evolution, the two clades of\n                    <jats:italic>Trichinella<\/jats:italic>\n                    exhibit differential expansion and methylation of parasitism-related multi-copy gene families, especially for the DNase II members of the phospholipase D superfamily and Glutathione S-transferases. Further, methylome and transcriptome analyses revealed divergent key excretory\/secretory (E\/S) genes between the two clades. Among these key E\/S genes, TP12446 is significantly more expressed across three life stages in\n                    <jats:italic>T. pseudospiralis<\/jats:italic>\n                    . Overexpression of TP12446 in the mouse C2C12 skeletal muscle cell line could induce inhibition of myotube formation and differentiation, further indicating its key role in parasitism of\n                    <jats:italic>T. pseudospiralis<\/jats:italic>\n                    . This multi-omics study provides a foundation for further elucidation of the mechanism of nurse cell formation and immunoevasion, as well as the identification of pharmacological and diagnostic targets of trichinellosis.\n                  <\/jats:p>","DOI":"10.1038\/s42003-021-01650-z","type":"journal-article","created":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T06:19:10Z","timestamp":1611901150000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparative multi-omics analyses reveal differential expression of key genes relevant for parasitism between non-encapsulated and encapsulated Trichinella"],"prefix":"10.1038","volume":"4","author":[{"given":"Xiaolei","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3932-0779","authenticated-orcid":false,"given":"Yayan","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9267-1105","authenticated-orcid":false,"given":"Mingyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8026-879X","authenticated-orcid":false,"given":"Fei","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,29]]},"reference":[{"key":"1650_CR1","doi-asserted-by":"publisher","first-page":"7354","DOI":"10.1073\/pnas.0602466103","volume":"103","author":"DS Zarlenga","year":"2006","unstructured":"Zarlenga, D. 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