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One srRF is an ICT1 protein from humans that rescues a \u201cnon-stop\u201d type of mitochondrial ribosomes (mitoribosomes) stalled on mRNA lacking a stop codon, while the other, C12orf65, reportedly has functions that overlap with those of ICT1; however, its primary role remains unclear. We herein demonstrated that the <jats:italic>Saccharomyces cerevisiae<\/jats:italic> homolog of C12orf65, Pth3 (Rso55), preferentially rescued antibiotic-dependent stalled mitoribosomes, which appear to represent a \u201cno-go\u201d type of ribosomes stalled on intact mRNA. On media containing a non-fermentable carbon source, which requires mitochondrial gene expression, respiratory growth was impaired significantly more by the deletion of <jats:italic>PTH3<\/jats:italic> than that of the <jats:italic>ICT1<\/jats:italic> homolog <jats:italic>PTH4<\/jats:italic> in the presence of antibiotics that inhibit mitochondrial translation, such as tetracyclines and macrolides. Additionally, the in organello labeling of mitochondrial translation products and quantification of mRNA levels by quantitative RT-PCR suggested that in the presence of tetracycline, the deletion of <jats:italic>PTH3<\/jats:italic>, but not <jats:italic>PTH4<\/jats:italic>, reduced the protein expression of all eight mtDNA-encoded genes at the post-transcriptional or translational level. These results indicate that Pth3 can function as a mitochondrial srRF specific for ribosomes stalled by antibiotics and plays a role in antibiotic resistance in fungi.<\/jats:p>","DOI":"10.1038\/s42003-021-01835-6","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T11:03:05Z","timestamp":1615201385000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A stalled-ribosome rescue factor Pth3 is required for mitochondrial translation against antibiotics in Saccharomyces cerevisiae"],"prefix":"10.1038","volume":"4","author":[{"given":"Soichiro","family":"Hoshino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryohei","family":"Kanemura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Kurita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiro","family":"Soutome","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyouta","family":"Himeno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1279-9505","authenticated-orcid":false,"given":"Masak","family":"Takaine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5744-197X","authenticated-orcid":false,"given":"Masakatsu","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6222-4451","authenticated-orcid":false,"given":"Nobukazu","family":"Nameki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"1835_CR1","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1111\/j.1365-2958.2005.04832.x","volume":"58","author":"SD Moore","year":"2005","unstructured":"Moore, S. 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