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In the malaria parasite <jats:italic>Plasmodium falciparum<\/jats:italic>, PfCK2 is vital for asexual proliferation of blood-stage parasites. Here, we applied CRISPR\/Cas9-based gene editing to investigate the function of the PfCK2\u03b1 catalytic subunit in gametocytes, the sexual forms of the parasite that are essential for malaria transmission. We show that PfCK2\u03b1 localizes to the nucleus and cytoplasm in asexual and sexual parasites alike. Conditional knockdown of PfCK2\u03b1 expression prevented the transition of stage IV into transmission-competent stage V gametocytes, whereas the conditional knockout of <jats:italic>pfck2a<\/jats:italic> completely blocked gametocyte maturation already at an earlier stage of sexual differentiation. In summary, our results demonstrate that PfCK2\u03b1 is not only essential for asexual but also sexual development of <jats:italic>P. falciparum<\/jats:italic> blood-stage parasites and encourage studies exploring PfCK2\u03b1 as a potential target for dual-active antimalarial drugs.<\/jats:p>","DOI":"10.1038\/s42003-021-01873-0","type":"journal-article","created":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T11:07:02Z","timestamp":1615547222000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["The catalytic subunit of Plasmodium falciparum casein kinase 2 is essential for gametocytogenesis"],"prefix":"10.1038","volume":"4","author":[{"given":"Eva","family":"Hitz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Gr\u00fcninger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armin","family":"Passecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Wyss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Scheurer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Wittlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Peter","family":"Beck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas M. 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