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Here we show using flow cytometry that sLe<jats:sup>x<\/jats:sup>expression on basophils and mast cell progenitors depends on fucosyltransferase 6 (<jats:italic>FUT6<\/jats:italic>). Using genetic association data analysis and qPCR, the cell type-specific defect was associated with single nucleotide polymorphisms (SNPs) in the<jats:italic>FUT6<\/jats:italic>gene region (tagged by rs17855739 and rs778798), affecting coding sequence and\/or expression level of the mRNA. Heterozygous individuals with one functional<jats:italic>FUT6<\/jats:italic>gene harbor a mixed population of sLe<jats:sup>x+<\/jats:sup>and sLe<jats:sup>x-<\/jats:sup>basophils, a phenomenon caused by random monoallelic expression (RME). Microfluidic assay demonstrated<jats:italic>FUT6<\/jats:italic>-deficient basophils rolling on E-selectin is severely impaired.<jats:italic>FUT6<\/jats:italic>null alleles carriers exhibit elevated blood basophil counts and a reduced itch sensitivity against insect bites.<jats:italic>FUT6<\/jats:italic>-deficiency thus dampens the basophil-mediated allergic response in the periphery, evident also in lower IgE titers and reduced eosinophil counts.<\/jats:p>","DOI":"10.1038\/s42003-021-02295-8","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T10:21:22Z","timestamp":1625221282000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["FUT6 deficiency compromises basophil function by selectively abrogating their sialyl-Lewis x expression"],"prefix":"10.1038","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2445-3154","authenticated-orcid":false,"given":"Kia 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