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The <jats:italic>Plasmodium falciparum Pfs47<\/jats:italic> gene has strong geographic population structure, because only those parasites with <jats:italic>Pfs47<\/jats:italic> haplotypes compatible with the mosquito vector species in a given continent are efficiently transmitted. Analysis of 4,971 world-wide <jats:italic>Pfs47<\/jats:italic> sequences identified two SNPs (at 707 and 725\u2009bp) as sufficient to establish the likely continent of origin of <jats:italic>P. falciparum<\/jats:italic> isolates. <jats:italic>Pfs47<\/jats:italic> sequences from Africa, Asia, and the New World presented more that 99% frequency of distinct combinations of the SNPs 707 and 725 genotypes. Interestingly, Papua New Guinea <jats:italic>Pfs47<\/jats:italic> sequences have the highest diversity in SNPs 707 and 725. Accurate and reproducible High-Resolution Melting (HRM) assays were developed to genotype <jats:italic>Pfs47<\/jats:italic> SNPs 707 and 725 in laboratory and field samples, to assess the geographic origin and risk of local transmission of imported <jats:italic>P. falciparum<\/jats:italic> malaria cases.<\/jats:p>","DOI":"10.1038\/s42003-021-02667-0","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T10:30:41Z","timestamp":1632997841000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases"],"prefix":"10.1038","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5137-4836","authenticated-orcid":false,"given":"Alvaro","family":"Molina-Cruz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9973-4391","authenticated-orcid":false,"given":"Nadia","family":"Raytselis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roxanne","family":"Withers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ankit","family":"Dwivedi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter D.","family":"Crompton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boubacar","family":"Traore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Carpi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6502-7026","authenticated-orcid":false,"given":"Joana C.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4039-6199","authenticated-orcid":false,"given":"Carolina","family":"Barillas-Mury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,9,30]]},"reference":[{"key":"2667_CR1","unstructured":"WHO. 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