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In this study, we generate\n                    <jats:italic>RhoGDI\u03b1<\/jats:italic>\n                    knockout mice, and find that the males have severely low fertility. The testes from\n                    <jats:italic>RhoGDI\u03b1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice are smaller than that in WT mice. The numbers of spermatogonia and spermatocytes are decreased in\n                    <jats:italic>RhoGDI\u03b1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    testis. Spermatogenesis is compromised, and spermatocyte meiosis is arrested at zygotene stage in\n                    <jats:italic>RhoGDI\u03b1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice. Acrosome dysplasia is also observed in sperms of the mutant mice. At the molecular level,\n                    <jats:italic>RhoGDI\u03b1<\/jats:italic>\n                    deficiency activate the LIMK\/cofilin signaling pathway, inhibiting F-actin depolymerization, impairing testis and inducing low fertility in mouse. In addition, the treatment of\n                    <jats:italic>RhoGDI\u03b1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice with Rac1 inhibitor NSC23766 alleviate testis injury and improve sperm quality by inhibiting the LIMK\/cofilin\/F-actin pathway during spermatogenesis. Together, these findings reveal a previously unrecognized RhoGDI\u03b1\/Rac1\/F-actin-dependent mechanism involved in spermatogenesis and male fertility.\n                  <\/jats:p>","DOI":"10.1038\/s42003-023-04579-7","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T09:04:50Z","timestamp":1677143090000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["RhoGDI\u03b1 regulates spermatogenesis through Rac1\/cofilin\/F-actin signaling"],"prefix":"10.1038","volume":"6","author":[{"given":"Haixia","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongzhuang","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aizhen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyue","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9578-8790","authenticated-orcid":false,"given":"Daqing","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3137-6804","authenticated-orcid":false,"given":"Bin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6619-7107","authenticated-orcid":false,"given":"Jiangang","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,2,23]]},"reference":[{"key":"4579_CR1","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.semcdb.2016.04.009","volume":"59","author":"FT Neto","year":"2016","unstructured":"Neto, F. 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