{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:47:26Z","timestamp":1790858846482,"version":"4.1.0"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T00:00:00Z","timestamp":1678406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T00:00:00Z","timestamp":1678406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"IZKF program of the Medical Faculty at the University of T\u00fcbingen"},{"name":"DFG Cluster of Excellence EXC2124 'Controlling Microbes to Fight Infection'"},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BO 1527\/4-1"],"award-info":[{"award-number":["BO 1527\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BO 1527\/4-1"],"award-info":[{"award-number":["BO 1527\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"DFG Cluster of Excellence EXC2124 'Controlling Microbes to Fight Infection'"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Commun Biol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    YgfB-mediated \u03b2-lactam resistance was recently identified in multi drug resistant\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    . We show that YgfB upregulates expression of the \u03b2-lactamase AmpC by repressing the function of the regulator of the programmed cell death pathway AlpA. In response to DNA damage, the antiterminator AlpA induces expression of the\n                    <jats:italic>alpBCDE<\/jats:italic>\n                    autolysis genes and of the peptidoglycan amidase AmpDh3. YgfB interacts with AlpA and represses the\n                    <jats:italic>ampDh3<\/jats:italic>\n                    expression. Thus, YgfB indirectly prevents AmpDh3 from reducing the levels of cell wall-derived 1,6-anhydro-N-acetylmuramyl-peptides, required to induce the transcriptional activator AmpR in promoting the\n                    <jats:italic>ampC<\/jats:italic>\n                    expression and \u03b2-lactam resistance. Ciprofloxacin-mediated DNA damage induces AlpA-dependent production of AmpDh3 as previously shown, which should reduce \u03b2-lactam resistance. YgfB, however, counteracts the \u03b2-lactam enhancing activity of ciprofloxacin by repressing\n                    <jats:italic>ampDh3<\/jats:italic>\n                    expression and lowering the benefits of this drug combination. Altogether, YgfB represents an additional player in the complex regulatory network of AmpC regulation.\n                  <\/jats:p>","DOI":"10.1038\/s42003-023-04609-4","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T23:34:24Z","timestamp":1678404864000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["YgfB increases \u03b2-lactam resistance in Pseudomonas aeruginosa by counteracting AlpA-mediated ampDh3 expression"],"prefix":"10.1038","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3701-3078","authenticated-orcid":false,"given":"Ole","family":"Eggers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8233-0916","authenticated-orcid":false,"given":"Fabian A.","family":"Renschler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia Anita","family":"Michalek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelle","family":"Wackler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elias","family":"Walter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Smollich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"Sonnabend","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Egle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Angelov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Engesser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Borisova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4731-4851","authenticated-orcid":false,"given":"Christoph","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Sch\u00fctz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0780-862X","authenticated-orcid":false,"given":"Erwin","family":"Bohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,10]]},"reference":[{"key":"4609_CR1","doi-asserted-by":"publisher","first-page":"636","DOI":"10.1128\/jb.152.2.636-642.1982","volume":"152","author":"F Yoshimura","year":"1982","unstructured":"Yoshimura, F. & Nikaido, H. Permeability of Pseudomonas aeruginosa outer membrane to hydrophilic solutes. J. Bacteriol. 152, 636\u2013642 (1982).","journal-title":"J. Bacteriol."},{"key":"4609_CR2","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.1099\/jmm.0.009142-0","volume":"58","author":"T Strateva","year":"2009","unstructured":"Strateva, T. & Yordanov, D. Pseudomonas aeruginosa\u2014a phenomenon of bacterial resistance. J. Med. Microbiol. 58, 1133\u20131148 (2009).","journal-title":"J. Med. Microbiol."},{"key":"4609_CR3","doi-asserted-by":"publisher","first-page":"3922","DOI":"10.1128\/AAC.00341-08","volume":"52","author":"AJ Schmidtke","year":"2008","unstructured":"Schmidtke, A. J. & Hanson, N. D. Role of ampD homologs in overproduction of AmpC in clinical isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52, 3922\u20133927 (2008).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR4","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1111\/j.1469-0691.2006.01674.x","volume":"13","author":"VH Tam","year":"2007","unstructured":"Tam, V. H. et al. Prevalence of AmpC over-expression in bloodstream isolates of Pseudomonas aeruginosa. Clin. Microbiol Infect. 13, 413\u2013418 (2007).","journal-title":"Clin. Microbiol Infect."},{"key":"4609_CR5","doi-asserted-by":"publisher","first-page":"e1000353","DOI":"10.1371\/journal.ppat.1000353","volume":"5","author":"B Moya","year":"2009","unstructured":"Moya, B. et al. Beta-lactam resistance response triggered by inactivation of a nonessential penicillin-binding protein. PLoS Pathog. 5, e1000353 (2009).","journal-title":"PLoS Pathog."},{"key":"4609_CR6","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1093\/infdis\/154.5.792","volume":"154","author":"CC Sanders","year":"1986","unstructured":"Sanders, C. C. & Sanders, W. E. Jr Type I beta-lactamases of gram-negative bacteria: interactions with beta-lactam antibiotics. J. Infect. Dis. 154, 792\u2013800 (1986).","journal-title":"J. Infect. Dis."},{"key":"4609_CR7","doi-asserted-by":"publisher","first-page":"e00524","DOI":"10.1128\/mSystems.00524-19","volume":"4","author":"G Torrens","year":"2019","unstructured":"Torrens, G. et al. Regulation of AmpC-Driven beta-Lactam Resistance in Pseudomonas aeruginosa: Different Pathways, Different Signaling. mSystems 4, e00524\u201319 (2019).","journal-title":"mSystems"},{"key":"4609_CR8","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1016\/S0092-8674(00)81928-5","volume":"88","author":"C Jacobs","year":"1997","unstructured":"Jacobs, C., Frere, J. M. & Normark, S. Cytosolic intermediates for cell wall biosynthesis and degradation control inducible beta-lactam resistance in gram-negative bacteria. Cell 88, 823\u2013832 (1997).","journal-title":"Cell"},{"key":"4609_CR9","doi-asserted-by":"publisher","first-page":"881","DOI":"10.2174\/1381612805666230112191507","volume":"5","author":"ND Hanson","year":"1999","unstructured":"Hanson, N. D. & Sanders, C. C. Regulation of inducible AmpC beta-lactamase expression among Enterobacteriaceae. Curr. Pharm. Des. 5, 881\u2013894 (1999).","journal-title":"Curr. Pharm. Des."},{"key":"4609_CR10","doi-asserted-by":"publisher","first-page":"e01771","DOI":"10.1128\/AAC.01771-19","volume":"64","author":"MS Sonnabend","year":"2020","unstructured":"Sonnabend, M. S. et al. Identification of Drug Resistance Determinants in a Clinical Isolate of Pseudomonas aeruginosa by High-Density Transposon Mutagenesis. Antimicrob. Agents Chemother. 64, e01771\u201319 (2020).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR11","doi-asserted-by":"publisher","unstructured":"Willmann, M. et al. Multi-omics approach identifies novel pathogen-derived prognostic biomarkers in patients with Pseudomonas aeruginosa bloodstream infection. bioRxiv 309898 https:\/\/doi.org\/10.1101\/309898 (2018).","DOI":"10.1101\/309898"},{"key":"4609_CR12","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1002\/prot.20260","volume":"57","author":"A Galkin","year":"2004","unstructured":"Galkin, A., Sarikaya, E., Lehmann, C., Howard, A. & Herzberg, O. X-ray structure of HI0817 from Haemophilus influenzae: protein of unknown function with a novel fold. Proteins 57, 874\u2013877 (2004).","journal-title":"Proteins"},{"key":"4609_CR13","doi-asserted-by":"publisher","first-page":"4950","DOI":"10.1021\/ja400970n","volume":"135","author":"W Zhang","year":"2013","unstructured":"Zhang, W. et al. Reactions of the three AmpD enzymes of Pseudomonas aeruginosa. J. Am. Chem. Soc. 135, 4950\u20134953 (2013).","journal-title":"J. Am. Chem. Soc."},{"key":"4609_CR14","doi-asserted-by":"publisher","first-page":"3694","DOI":"10.1128\/AAC.00172-08","volume":"52","author":"B Moya","year":"2008","unstructured":"Moya, B., Juan, C., Alberti, S., Perez, J. L. & Oliver, A. Benefit of having multiple ampD genes for acquiring beta-lactam resistance without losing fitness and virulence in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52, 3694\u20133700 (2008).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR15","doi-asserted-by":"publisher","first-page":"8433","DOI":"10.1073\/pnas.1506299112","volume":"112","author":"KA McFarland","year":"2015","unstructured":"McFarland, K. A. et al. A self-lysis pathway that enhances the virulence of a pathogenic bacterium. Proc. Natl. Acad. Sci. USA 112, 8433\u20138438 (2015).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"4609_CR16","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21941-7","volume":"12","author":"JM Pena","year":"2021","unstructured":"Pe\u00f1a, J. M. et al. Control of a programmed cell death pathway in Pseudomonas aeruginosa by an antiterminator. Nat. Commun. 12, 1702 (2021).","journal-title":"Nat. Commun."},{"key":"4609_CR17","doi-asserted-by":"publisher","first-page":"8321","DOI":"10.1093\/nar\/gkac608","volume":"50","author":"A Wen","year":"2022","unstructured":"Wen, A., Zhao, M., Jin, S., Lu, Y. Q. & Feng, Y. Structural basis of AlpA-dependent transcription antitermination. Nucleic Acids Res. 50, 8321\u20138330 (2022).","journal-title":"Nucleic Acids Res."},{"key":"4609_CR18","doi-asserted-by":"publisher","first-page":"1240","DOI":"10.1111\/mmi.14490","volume":"113","author":"S Westerhausen","year":"2020","unstructured":"Westerhausen, S. et al. A NanoLuc luciferase-based assay enabling the real-time analysis of protein secretion and injection by bacterial type III secretion systems. Mol. Microbiol 113, 1240\u20131254 (2020).","journal-title":"Mol. Microbiol"},{"key":"4609_CR19","doi-asserted-by":"publisher","first-page":"4684","DOI":"10.1002\/j.1460-2075.1994.tb06792.x","volume":"13","author":"C Jacobs","year":"1994","unstructured":"Jacobs, C., Huang, L. J., Bartowsky, E., Normark, S. & Park, J. T. Bacterial cell wall recycling provides cytosolic muropeptides as effectors for beta-lactamase induction. EMBO J. 13, 4684\u20134694 (1994).","journal-title":"EMBO J."},{"key":"4609_CR20","doi-asserted-by":"publisher","unstructured":"Colautti, J., Bullen, N. P. & Whitney, J. C. Lack of evidence that Pseudomonas aeruginosa AmpDh3-PA0808 constitute a type VI secretion system effector-immunity pair. Mol. Microbiol. https:\/\/doi.org\/10.1111\/mmi.15021 (2022). Epub ahead of print.","DOI":"10.1111\/mmi.15021"},{"key":"4609_CR21","doi-asserted-by":"publisher","first-page":"11","DOI":"10.4161\/rna.8.1.13346","volume":"8","author":"M Naville","year":"2011","unstructured":"Naville, M., Ghuillot-Gaudeffroy, A., Marchais, A. & Gautheret, D. ARNold: a web tool for the prediction of Rho-independent transcription terminators. RNA Biol. 8, 11\u201313 (2011).","journal-title":"RNA Biol."},{"key":"4609_CR22","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1089\/mdr.2016.0083","volume":"22","author":"I Rivera","year":"2016","unstructured":"Rivera, I., Molina, R., Lee, M., Mobashery, S. & Hermoso, J. A. Orthologous and Paralogous AmpD Peptidoglycan Amidases from Gram-Negative Bacteria. Micro. Drug Resist 22, 470\u2013476 (2016).","journal-title":"Micro. Drug Resist"},{"key":"4609_CR23","doi-asserted-by":"publisher","first-page":"1780","DOI":"10.1128\/AAC.50.5.1780-1787.2006","volume":"50","author":"C Juan","year":"2006","unstructured":"Juan, C., Moya, B., Perez, J. L. & Oliver, A. Stepwise upregulation of the Pseudomonas aeruginosa chromosomal cephalosporinase conferring high-level beta-lactam resistance involves three AmpD homologues. Antimicrob. Agents Chemother. 50, 1780\u20131787 (2006).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR24","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1128\/AAC.34.3.487","volume":"34","author":"JA Bosso","year":"1990","unstructured":"Bosso, J. A., Saxon, B. A. & Matsen, J. M. In vitro activities of combinations of aztreonam, ciprofloxacin, and ceftazidime against clinical isolates of Pseudomonas aeruginosa and Pseudomonas cepacia from patients with cystic fibrosis. Antimicrob. Agents Chemother. 34, 487\u2013488 (1990).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR25","doi-asserted-by":"publisher","first-page":"e01150","DOI":"10.1128\/AAC.01150-18","volume":"62","author":"VE Rees","year":"2018","unstructured":"Rees, V. E. et al. Meropenem combined with ciprofloxacin combats hypermutable Pseudomonas aeruginosa from respiratory infections of cystic fibrosis patients. Antimicrob. Agents Chemother. 62, e01150\u201318 (2018).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR26","doi-asserted-by":"publisher","first-page":"1814","DOI":"10.1128\/AAC.34.9.1814","volume":"34","author":"CI Bustamante","year":"1990","unstructured":"Bustamante, C. I., Wharton, R. C. & Wade, J. C. In vitro activity of ciprofloxacin in combination with ceftazidime, aztreonam, and azlocillin against multiresistant isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 34, 1814\u20131815 (1990).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR27","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.ijantimicag.2008.02.019","volume":"32","author":"K Kanellakopoulou","year":"2008","unstructured":"Kanellakopoulou, K., Sarafis, P., Galani, I., Giamarellou, H. & Giamarellos-Bourboulis, E. J. In vitro synergism of beta-lactams with ciprofloxacin and moxifloxacin against genetically distinct multidrug-resistant isolates of Pseudomonas aeruginosa. Int. J. Antimicrob. agents 32, 33\u201339 (2008).","journal-title":"Int. J. Antimicrob. agents"},{"key":"4609_CR28","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1093\/infdis\/137.2.122","volume":"137","author":"MC Berenbaum","year":"1978","unstructured":"Berenbaum, M. C. A method for testing for synergy with any number of agents. J. Infect. Dis. 137, 122\u2013130 (1978).","journal-title":"J. Infect. Dis."},{"key":"4609_CR29","doi-asserted-by":"publisher","first-page":"1691","DOI":"10.1128\/AAC.39.8.1691","volume":"39","author":"MH Nguyen","year":"1995","unstructured":"Nguyen, M. H., Barchiesi, F., McGough, D. A., Yu, V. L. & Rinaldi, M. G. In vitro evaluation of combination of fluconazole and flucytosine against Cryptococcus neoformans var. neoformans. Antimicrob. Agents Chemother. 39, 1691\u20131695 (1995).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR30","doi-asserted-by":"publisher","first-page":"1361","DOI":"10.1128\/AAC.39.6.1361","volume":"39","author":"TJ Walsh","year":"1995","unstructured":"Walsh, T. J. et al. Activities of amphotericin B and antifungal azoles alone and in combination against Pseudallescheria boydii. Antimicrob. Agents Chemother. 39, 1361\u20131364 (1995).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR31","doi-asserted-by":"publisher","first-page":"1914","DOI":"10.1128\/AAC.40.8.1914","volume":"40","author":"RL White","year":"1996","unstructured":"White, R. L., Burgess, D. S., Manduru, M. & Bosso, J. A. Comparison of three different in vitro methods of detecting synergy: time-kill, checkerboard, and E test. Antimicrob. Agents Chemother. 40, 1914\u20131918 (1996).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR32","doi-asserted-by":"publisher","first-page":"4678","DOI":"10.1128\/AAC.00497-10","volume":"54","author":"MM Sopirala","year":"2010","unstructured":"Sopirala, M. M. et al. Synergy testing by E test, microdilution checkerboard, and time-kill methods for pan-drug-resistant Acinetobacter baumannii. Antimicrob. Agents Chemother. 54, 4678\u20134683 (2010).","journal-title":"Antimicrob. Agents Chemother."},{"key":"4609_CR33","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1038\/nmeth.1318","volume":"6","author":"DG Gibson","year":"2009","unstructured":"Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343\u2013345 (2009).","journal-title":"Nat. Methods"},{"key":"4609_CR34","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2019.00100","volume":"10","author":"K Klein","year":"2019","unstructured":"Klein, K. et al. Deprivation of the periplasmic chaperone SurA reduces virulence and restores antibiotic susceptibility of multidrug-resistant Pseudomonas aeruginosa. Front. Microbiol. 10, 1\u201317 (2019).","journal-title":"Front. Microbiol."},{"key":"4609_CR35","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1038\/nprot.2006.24","volume":"1","author":"KH Choi","year":"2006","unstructured":"Choi, K. H. & Schweizer, H. P. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1, 153\u2013161 (2006).","journal-title":"Nat. Protoc."},{"key":"4609_CR36","doi-asserted-by":"publisher","first-page":"6715","DOI":"10.1128\/AEM.02041-16","volume":"82","author":"J Meisner","year":"2016","unstructured":"Meisner, J. & Goldberg, J. B. The Escherichia coli rhaSR-PrhaBAD inducible promoter system allows tightly controlled gene expression over a wide range in Pseudomonas aeruginosa. Appl Environ. Microbiol 82, 6715\u20136727 (2016).","journal-title":"Appl Environ. Microbiol"},{"key":"4609_CR37","doi-asserted-by":"publisher","first-page":"e47","DOI":"10.1093\/nar\/gkz114","volume":"47","author":"Y Liao","year":"2019","unstructured":"Liao, Y., Smyth, G. K. & Shi, W. The R package Rsubread is easier, faster, cheaper and better for alignment and quantification of RNA sequencing reads. Nucleic Acids Res. 47, e47 (2019).","journal-title":"Nucleic Acids Res."},{"key":"4609_CR38","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 550 (2014).","journal-title":"Genome Biol."},{"key":"4609_CR39","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1016\/j.mimet.2005.06.001","volume":"64","author":"KH Choi","year":"2006","unstructured":"Choi, K. H., Kumar, A. & Schweizer, H. P. A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. J. Microbiol. Methods 64, 391\u2013397 (2006).","journal-title":"J. Microbiol. Methods"},{"key":"4609_CR40","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1038\/nchembio.1289","volume":"9","author":"J Gisin","year":"2013","unstructured":"Gisin, J., Schneider, A., Nagele, B., Borisova, M. & Mayer, C. A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis. Nat. Chem. Biol. 9, 491\u2013493 (2013).","journal-title":"Nat. Chem. Biol."},{"key":"4609_CR41","unstructured":"Clinical and Laboratory Standards Institute; Wayne, PA: CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 28th Eds. CLSI Guideline M100. (2018)."},{"key":"4609_CR42","doi-asserted-by":"publisher","first-page":"e1006071","DOI":"10.1371\/journal.ppat.1006071","volume":"12","author":"T Dietsche","year":"2016","unstructured":"Dietsche, T. et al. Structural and functional characterization of the bacterial type III secretion export apparatus. PLoS Pathog. 12, e1006071 (2016).","journal-title":"PLoS Pathog."},{"key":"4609_CR43","doi-asserted-by":"crossref","unstructured":"Wang, T. et al. A type VI secretion system delivers a cell wall amidase to target bacterial competitors. Mol. Microbiol. 114, 308\u2013321 (2020).","DOI":"10.1111\/mmi.14513"},{"key":"4609_CR44","unstructured":"Eggers, O. et al. Transcriptomic analysis comparing ID40 WT with ygfB-deletion mutant. National Center for Biotechnology Information. https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA835697 (2022)."},{"key":"4609_CR45","unstructured":"Sonnabend, M. S. et al. Pseudomonas aeruginosa isolate ID40 genome assembly, chromosome: ID40_chromosome. European Nucleotide Archive. https:\/\/www.ebi.ac.uk\/ena\/browser\/view\/LR700248 (2019)."}],"container-title":["Communications Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s42003-023-04609-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-023-04609-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-023-04609-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T23:35:14Z","timestamp":1678404914000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s42003-023-04609-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,10]]},"references-count":45,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["4609"],"URL":"https:\/\/doi.org\/10.1038\/s42003-023-04609-4","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.07.05.498809","asserted-by":"object"}]},"ISSN":["2399-3642"],"issn-type":[{"value":"2399-3642","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,10]]},"assertion":[{"value":"5 July 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 February 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 March 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"254"}}