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Here we modelled the longitudinal expression of endogenous\n                    <jats:italic>Dmd<\/jats:italic>\n                    , and its paralogue\n                    <jats:italic>Utrn<\/jats:italic>\n                    , in mice and in myoblasts by generating bespoke bioluminescent gene reporters. As utrophin can partially compensate for\n                    <jats:italic>Dmd<\/jats:italic>\n                    -deficiency, these reporters were used as tools to ask whether chromatin-modifying drugs can enhance\n                    <jats:italic>Utrn<\/jats:italic>\n                    expression in developing muscle. Myoblasts treated with different PRC2 inhibitors showed significant increases in\n                    <jats:italic>Utrn<\/jats:italic>\n                    transcripts and bioluminescent signals, and these responses were independently verified by conditional\n                    <jats:italic>Ezh2<\/jats:italic>\n                    deletion. Inhibition of ERK1\/2 signalling provoked an additional increase in\n                    <jats:italic>Utrn<\/jats:italic>\n                    expression that was also seen in\n                    <jats:italic>Dmd-<\/jats:italic>\n                    mutant cells, and maintained as myoblasts differentiate. These data reveal PRC2 and ERK1\/2 to be negative regulators of\n                    <jats:italic>Utrn<\/jats:italic>\n                    expression and provide specialised molecular imaging tools to monitor utrophin expression as a therapeutic strategy for DMD.\n                  <\/jats:p>","DOI":"10.1038\/s42003-023-04666-9","type":"journal-article","created":{"date-parts":[[2023,3,25]],"date-time":"2023-03-25T01:02:41Z","timestamp":1679706161000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Endogenous bioluminescent reporters reveal a sustained increase in utrophin gene expression upon EZH2 and ERK1\/2 inhibition"],"prefix":"10.1038","volume":"6","author":[{"given":"Hannah J.","family":"Gleneadie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Fernandez-Ruiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Sardini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathew","family":"Van de Pette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2996-2479","authenticated-orcid":false,"given":"Andrew","family":"Dimond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2666-3326","authenticated-orcid":false,"given":"Rab K.","family":"Prinjha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"McGinty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0478-6755","authenticated-orcid":false,"given":"Paul M. 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