{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:53:37Z","timestamp":1789588417627,"version":"build-2803163510"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Commun Biol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Zebrafish (\n                    <jats:italic>Danio rerio<\/jats:italic>\n                    ) is a commonly-used vertebrate model species for many research areas. However, its low milt volume limits effective cryopreservation of sperm from a single individual and often precludes dividing a single semen sample to conduct multiple downstream procedures such as genomic DNA\/RNA extraction and in-vitro fertilization. Here, we apply germ stem cell transplantation to increase zebrafish sperm production in a closely related larger species from the same subfamily, giant danio\n                    <jats:italic>Devario aequipinnatus<\/jats:italic>\n                    . The endogenous germ cell of the host is depleted by dead-end morpholino antisense oligonucleotide. Histology of the sterile gonad and quantitative PCR of gonadal tissue reveals all sterile giant danio develop the male phenotype. Spermatogonial cells of Tg(ddx4:egfp) transgenic zebrafish are transplanted into sterile giant danio larvae, and 22% of recipients (germline chimera) produce donor-derived sperm at sexual maturation. The germline chimera produce approximately three-fold the volume of sperm and 10-fold the spermatozoon concentration of the donor. The donor-derived sperm is functional and gives rise to viable progeny upon fertilization of donor oocytes. We show that the issue of low milt volume can be effectively addressed by employing a larger surrogate parent.\n                  <\/jats:p>","DOI":"10.1038\/s42003-023-04800-7","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T08:02:13Z","timestamp":1681459333000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Enhancement of zebrafish sperm production via a large body-sized surrogate with germ cell transplantation"],"prefix":"10.1038","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5327-801X","authenticated-orcid":false,"given":"Rigolin","family":"Nayak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Fran\u011bk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radek","family":"\u0160indelka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"P\u0161eni\u010dka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,4,14]]},"reference":[{"key":"4800_CR1","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/0734-9750(96)00004-3","volume":"14","author":"Z Lele","year":"1996","unstructured":"Lele, Z. & Krone, P. H. The zebrafish as a model system in developmental, toxicological and transgenic research. Biotechnol. Adv. 14, 57\u201372 (1996).","journal-title":"Biotechnol. Adv."},{"key":"4800_CR2","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1038\/nrg2091","volume":"8","author":"GJ Lieschke","year":"2007","unstructured":"Lieschke, G. J. & Currie, P. D. Animal models of human disease: zebrafish swim into view. Nat. Rev. Genet. 8, 353\u2013367 (2007).","journal-title":"Nat. Rev. Genet."},{"key":"4800_CR3","doi-asserted-by":"crossref","unstructured":"Briggs, J. P. The zebrafish: a new model organism for integrative physiology. Am. J. Physiol. Regul. Integr. Comp. Physiol. 282, (2002).","DOI":"10.1152\/ajpregu.00589.2001"},{"key":"4800_CR4","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1203\/PDR.0b013e318186e609","volume":"64","author":"MB Veldman","year":"2008","unstructured":"Veldman, M. B. & Lin, S. Zebrafish as a developmental model organism for pediatric research. Pediatr. Res. 64, 470\u2013476 (2008).","journal-title":"Pediatr. Res."},{"key":"4800_CR5","doi-asserted-by":"publisher","first-page":"e21059","DOI":"10.1371\/journal.pone.0021059","volume":"6","author":"M Hagedorn","year":"2011","unstructured":"Hagedorn, M. & Carter, V. L. Zebrafish reproduction: revisiting in vitro fertilization to increase sperm cryopreservation success. PLoS ONE 6, e21059 (2011).","journal-title":"PLoS ONE"},{"key":"4800_CR6","doi-asserted-by":"publisher","first-page":"956","DOI":"10.2144\/03355st03","volume":"35","author":"JP Morris IV","year":"2003","unstructured":"Morris, J. P. IV et al. Zebrafish sperm cryopreservation with N,N-dimethylacetamide. Biotechniques 35, 956\u2013968 (2003).","journal-title":"Biotechniques"},{"key":"4800_CR7","doi-asserted-by":"publisher","unstructured":"Harvey, B., Kelley, R. N. & Ashwood-Smith, M. J. Cryopreservation of zebra fish spermatozoa using methanol. Can. J. Zool. https:\/\/doi.org\/10.1139\/z82-242 (2011).","DOI":"10.1139\/z82-242"},{"key":"4800_CR8","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.theriogenology.2007.02.015","volume":"68","author":"H Yang","year":"2007","unstructured":"Yang, H., Carmichael, C., Varga, Z. M. & Tiersch, T. R. Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio. Theriogenology 68, 128\u2013136 (2007).","journal-title":"Theriogenology"},{"key":"4800_CR9","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1016\/S0091-679X(04)77034-X","volume":"77","author":"S Berghmans","year":"2004","unstructured":"Berghmans, S., Morris, J. P. IV, Kanki, J. P. & Look, A. T. Zebrafish sperm cryopreservation. Methods Cell Biol. 77, 645\u2013659 (2004).","journal-title":"Methods Cell Biol."},{"key":"4800_CR10","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1152\/ajpregu.00905.2005","volume":"291","author":"PR Biga","year":"2006","unstructured":"Biga, P. R. & Goetz, F. W. Zebrafish and giant danio as models for muscle growth: determinate vs. indeterminate growth as determined by morphometric analysis. Am. J. Physiol. - Regul. Integr. Comp. Physiol. 291, 1327\u20131337 (2006).","journal-title":"Am. J. Physiol. - Regul. Integr. Comp. Physiol."},{"key":"4800_CR11","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.theriogenology.2019.03.032","volume":"133","author":"R Goto","year":"2019","unstructured":"Goto, R. & Saito, T. A state-of-the-art review of surrogate propagation in fish. Theriogenology 133, 216\u2013227 (2019).","journal-title":"Theriogenology"},{"key":"4800_CR12","doi-asserted-by":"publisher","first-page":"2051","DOI":"10.1071\/RD15136","volume":"28","author":"I Bar","year":"2016","unstructured":"Bar, I. et al. Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation. Reprod. Fertil. Dev. 28, 2051\u20132064 (2016).","journal-title":"Reprod. Fertil. Dev."},{"key":"4800_CR13","doi-asserted-by":"publisher","first-page":"737759","DOI":"10.1016\/j.aquaculture.2021.737759","volume":"549","author":"R Fran\u011bk","year":"2022","unstructured":"Fran\u011bk, R. et al. Who is the best surrogate for germ stem cell transplantation in fish? Aquaculture 549, 737759 (2022).","journal-title":"Aquaculture"},{"key":"4800_CR14","doi-asserted-by":"publisher","first-page":"1108","DOI":"10.1093\/biolre\/ioy236","volume":"100","author":"A Octavera","year":"2019","unstructured":"Octavera, A. & Yoshizaki, G. Production of donor-derived offspring by allogeneic transplantation of spermatogonia in Chinese rosy bitterling. Biol. Reprod. 100, 1108\u20131117 (2019).","journal-title":"Biol. Reprod."},{"key":"4800_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/srep43185","volume":"7","author":"S Seki","year":"2017","unstructured":"Seki, S. et al. Production of the medaka derived from vitrified whole testes by germ cell transplantation. Sci. Rep. 7, 1\u201311 (2017).","journal-title":"Sci. Rep."},{"key":"4800_CR16","doi-asserted-by":"publisher","first-page":"4519","DOI":"10.1073\/pnas.89.10.4519","volume":"89","author":"S Lin","year":"1992","unstructured":"Lin, S., Long, W., Chen, J. & Hopkins, N. Production of germ-line chimeras in zebrafish by cell transplants from genetically pigmented to albino embryos. Proc. Natl Acad. Sci. USA 89, 4519\u20134523 (1992).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"4800_CR17","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1007\/s10126-015-9657-5","volume":"17","author":"T Morita","year":"2015","unstructured":"Morita, T. et al. Functional sperm of the Yellowtail (Seriola quinqueradiata) were produced in the small-bodied surrogate, Jack Mackerel (Trachurus japonicus). Mar. Biotechnol. 17, 644\u2013654 (2015).","journal-title":"Mar. Biotechnol."},{"key":"4800_CR18","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1007\/s10126-017-9777-1","volume":"19","author":"M Hamasaki","year":"2017","unstructured":"Hamasaki, M. et al. Production of tiger puffer takifugu rubripes offspring from triploid grass puffer Takifugu niphobles parents. Mar. Biotechnol. 19, 579\u2013591 (2017).","journal-title":"Mar. Biotechnol."},{"key":"4800_CR19","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.aquaculture.2019.03.037","volume":"506","author":"RS Hattori","year":"2019","unstructured":"Hattori, R. S. et al. Surrogate production of Salmo salar oocytes and sperm in triploid Oncorhynchus mykiss by germ cell transplantation technology. Aquaculture 506, 238\u2013245 (2019).","journal-title":"Aquaculture"},{"key":"4800_CR20","doi-asserted-by":"crossref","unstructured":"Fran\u011bk, R., Ka\u0161par, V., Shah, M. A., Gela, D. & P\u0161eni\u010dka, M. Production of common carp donor-derived offspring from goldfish surrogate broodstock. Aquaculture 534, (2021).","DOI":"10.1016\/j.aquaculture.2020.736252"},{"key":"4800_CR21","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.cryobiol.2016.02.005","volume":"72","author":"M P\u0161eni\u010dka","year":"2016","unstructured":"P\u0161eni\u010dka, M., Saito, T., Rodina, M. & Dzyuba, B. Cryopreservation of early stage Siberian sturgeon Acipenser baerii germ cells, comparison of whole tissue and dissociated cells. Cryobiology 72, 119\u2013122 (2016).","journal-title":"Cryobiology"},{"key":"4800_CR22","doi-asserted-by":"publisher","first-page":"52","DOI":"10.2144\/00291bm09","volume":"29","author":"GE Truett","year":"2000","unstructured":"Truett, G. E. et al. Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT). Biotechniques 29, 52\u201354 (2000).","journal-title":"Biotechniques"},{"key":"4800_CR23","doi-asserted-by":"publisher","first-page":"4074","DOI":"10.1073\/pnas.0407475102","volume":"102","author":"K Slanchev","year":"2005","unstructured":"Slanchev, K., Stebler, J., De La Cueva-M\u00e9ndez, G. & Raz, E. Development without germ cells: the role of the germ line in zebrafish sex differentiation. Proc. Natl Acad. Sci. USA 102, 4074\u20134079 (2005).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"4800_CR24","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.ydbio.2008.09.025","volume":"324","author":"KR Siegfried","year":"2008","unstructured":"Siegfried, K. R. & N\u00fcsslein-Volhard, C. Germ line control of female sex determination in zebrafish. Dev. Biol. 324, 277\u2013287 (2008).","journal-title":"Dev. Biol."},{"key":"4800_CR25","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.stemcr.2014.10.011","volume":"4","author":"KW Tzung","year":"2015","unstructured":"Tzung, K. W. et al. Early depletion of primordial germ cells in Zebrafish promotes testis formation. Stem Cell Rep. 4, 61\u201373 (2015).","journal-title":"Stem Cell Rep."},{"key":"4800_CR26","first-page":"3","volume":"10","author":"S Kondracki","year":"2005","unstructured":"Kondracki, S., Bonaszewska, D. & Mielnicka, C. The effect of age on the morphometric sperm traits of domestic pigs (Sus scrofa domestica). Cell. Mol. Biol. Lett. 10, 3\u201313 (2005).","journal-title":"Cell. Mol. Biol. Lett."},{"key":"4800_CR27","doi-asserted-by":"publisher","first-page":"816","DOI":"10.1111\/j.1439-0426.2010.01548.x","volume":"26","author":"E Yamaha","year":"2010","unstructured":"Yamaha, E. et al. Primordial germ cell in teleost fish with special references to its specification and migration. J. Appl. Ichthyol. 26, 816\u2013822 (2010).","journal-title":"J. Appl. Ichthyol."},{"key":"4800_CR28","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1038\/nrg1154","volume":"4","author":"E Raz","year":"2003","unstructured":"Raz, E. Primordial germ-cell development: the zebrafish perspective. Nat. Rev. Genet. 4, 690\u2013700 (2003).","journal-title":"Nat. Rev. Genet."},{"key":"4800_CR29","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1023\/B:FISH.0000030628.91607.2d","volume":"28","author":"G Yoshizaki","year":"2003","unstructured":"Yoshizaki, G., Takeuchi, Y., Kobayashi, T. & Takeuchi, T. Primordial germ cell: a novel tool for fish bioengineering. Fish. Physiol. Biochem. 28, 453\u2013457 (2003).","journal-title":"Fish. Physiol. Biochem."},{"key":"4800_CR30","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1023\/A:1023388317621","volume":"26","author":"G Yoshizaki","year":"2002","unstructured":"Yoshizaki, G., Takeuchi, Y., Kobayashi, T., Ihara, S. & Takeuchi, T. Primordial germ cells: the blueprint for a piscine life. Fish. Physiol. Biochem. 26, 3\u201312 (2002).","journal-title":"Fish. Physiol. Biochem."},{"key":"4800_CR31","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/S0925-4773(02)00154-5","volume":"116","author":"AV Kr\u00f8vel","year":"2002","unstructured":"Kr\u00f8vel, A. V. & Olsen, L. C. Expression of a vas::EGFP transgene in primordial germ cells of the zebrafish. Mech. Dev. 116, 141\u2013150 (2002).","journal-title":"Mech. Dev."},{"key":"4800_CR32","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.theriogenology.2019.08.016","volume":"140","author":"R Fran\u011bk","year":"2019","unstructured":"Fran\u011bk, R., Tichop\u00e1d, T., Fu\u010d\u00edkov\u00e1, M., Steinbach, C. & P\u0161eni\u010dka, M. Production and use of triploid zebrafish for surrogate reproduction. Theriogenology 140, 33\u201343 (2019).","journal-title":"Theriogenology"},{"key":"4800_CR33","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.mce.2009.04.009","volume":"312","author":"E Clelland","year":"2009","unstructured":"Clelland, E. & Peng, C. Endocrine\/paracrine control of zebrafish ovarian development. Mol. Cell. Endocrinol. 312, 42\u201352 (2009).","journal-title":"Mol. Cell. Endocrinol."},{"key":"4800_CR34","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1530\/JOE-13-0488","volume":"221","author":"W He","year":"2014","unstructured":"He, W., Dai, X., Chen, X., He, J. & Yin, Z. Zebrafish pituitary gene expression before and after sexual maturation. J. Endocrinol. 221, 429\u2013440 (2014).","journal-title":"J. Endocrinol."},{"key":"4800_CR35","doi-asserted-by":"publisher","first-page":"1190","DOI":"10.1095\/biolreprod.110.088427","volume":"84","author":"TT Wong","year":"2011","unstructured":"Wong, T. T., Saito, T., Crodian, J. & Collodi, P. Zebrafish germline chimeras produced by transplantation of ovarian germ cells into sterile host larvae. Biol. Reprod. 84, 1190\u20131197 (2011).","journal-title":"Biol. Reprod."},{"key":"4800_CR36","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-50169-1","volume":"9","author":"Z Marinovi\u0107","year":"2019","unstructured":"Marinovi\u0107, Z. et al. Preservation of zebrafish genetic resources through testis cryopreservation and spermatogonia transplantation. Sci. Rep. 9, 13861 (2019).","journal-title":"Sci. Rep."},{"key":"4800_CR37","doi-asserted-by":"publisher","first-page":"1810","DOI":"10.1002\/mrd.23275","volume":"86","author":"K Ichida","year":"2019","unstructured":"Ichida, K. et al. Enrichment of transplantable germ cells in salmonids using a novel monoclonal antibody by magnetic-activated cell sorting. Mol. Reprod. Dev. 86, 1810\u20131821 (2019).","journal-title":"Mol. Reprod. Dev."},{"key":"4800_CR38","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1002\/mrd.22224","volume":"80","author":"R Yazawa","year":"2013","unstructured":"Yazawa, R., Takeuchi, Y., Morita, T., Ishida, M. & Yoshizaki, G. The Pacific bluefin tuna (Thunnus orientalis) dead end gene is suitable as a specific molecular marker of type A spermatogonia. Mol. Reprod. Dev. 80, 871\u2013880 (2013).","journal-title":"Mol. Reprod. Dev."},{"key":"4800_CR39","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1095\/biolreprod.107.064667","volume":"78","author":"A Yano","year":"2008","unstructured":"Yano, A., Suzuki, K. & Yoshizaki, G. Flow-cytometric isolation of testicular germ cells from rainbow Trout (Oncorhynchus mykiss) carrying the green fluorescent protein gene driven by Trout vasa regulatory regions. Biol. Reprod. 78, 151\u2013158 (2008).","journal-title":"Biol. Reprod."},{"key":"4800_CR40","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.ygcen.2004.07.003","volume":"139","author":"R Goto-Kazeto","year":"2004","unstructured":"Goto-Kazeto, R., Kight, K. E., Zohar, Y., Place, A. R. & Trant, J. M. Localization and expression of aromatase mRNA in adult zebrafish. Gen. Comp. Endocrinol. 139, 72\u201384 (2004).","journal-title":"Gen. Comp. Endocrinol."},{"key":"4800_CR41","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1016\/j.ygcen.2009.03.002","volume":"165","author":"Y Guiguen","year":"2010","unstructured":"Guiguen, Y., Fostier, A., Piferrer, F. & Chang, C. F. Ovarian aromatase and estrogens: A pivotal role for gonadal sex differentiation and sex change in fish. Gen. Comp. Endocrinol. 165, 352\u2013366 (2010).","journal-title":"Gen. Comp. Endocrinol."},{"key":"4800_CR42","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/S1532-0456(01)00267-8","volume":"130","author":"JF Baroiller","year":"2001","unstructured":"Baroiller, J. F. & D\u2019Cotta, H. Environment and sex determination in farmed fish. Comp. Biochem. Physiol. Part C. Toxicol. Pharmacol. 130, 399\u2013409 (2001).","journal-title":"Comp. Biochem. Physiol. Part C. Toxicol. Pharmacol."},{"key":"4800_CR43","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1038\/nature10239","volume":"476","author":"C Matson","year":"2011","unstructured":"Matson, C. et al. DMRT1 prevents female reprogramming in the postnatal mammalian testis. Nature 476, 101\u2013104 (2011).","journal-title":"Nature"},{"key":"4800_CR44","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1006\/dbio.2000.0129","volume":"231","author":"EFL Chiang","year":"2001","unstructured":"Chiang, E. F. L. et al. Two Sox9 genes on duplicated Zebrafish chromosomes: expression of similar transcription activators in distinct sites. Dev. Biol. 231, 149\u2013163 (2001).","journal-title":"Dev. Biol."},{"key":"4800_CR45","doi-asserted-by":"publisher","first-page":"16958","DOI":"10.1073\/pnas.0609932104","volume":"104","author":"H Kurokawa","year":"2007","unstructured":"Kurokawa, H. et al. Germ cells are essential for sexual dimorphism in the medaka gonad. Proc. Natl Acad. Sci. USA 104, 16958\u201316963 (2007).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"4800_CR46","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1007\/s10126-019-09874-1","volume":"21","author":"D Ye","year":"2019","unstructured":"Ye, D. et al. Abundance of early embryonic primordial germ cells promotes Zebrafish female differentiation as revealed by lifetime labeling of germline. Mar. Biotechnol. 21, 217\u2013228 (2019).","journal-title":"Mar. Biotechnol."},{"key":"4800_CR47","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1095\/biolreprod.107.060038","volume":"78","author":"T Saito","year":"2008","unstructured":"Saito, T., Goto-Kazeto, R., Arai, K. & Yamaha, E. Xenogenesis in teleost fish through generation of germ-line chimeras by single primordial germ cell transplantation. Biol. Reprod. 78, 159\u2013166 (2008).","journal-title":"Biol. Reprod."},{"key":"4800_CR48","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1007\/s11427-021-1989-9","volume":"65","author":"F Zhang","year":"2022","unstructured":"Zhang, F. et al. Surrogate production of genome-edited sperm from a different subfamily by spermatogonial stem cell transplantation. Sci. China Life Sci. 65, 969\u2013987 (2022).","journal-title":"Sci. China Life Sci."},{"key":"4800_CR49","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1126\/science.aao6868","volume":"360","author":"DR Barneche","year":"2018","unstructured":"Barneche, D. R., Ross Robertson, D., White, C. R. & Marshall, D. J. Fish reproductive-energy output increases disproportionately with body size. Science 360, 642\u2013645 (2018).","journal-title":"Science"},{"key":"4800_CR50","doi-asserted-by":"publisher","first-page":"16557","DOI":"10.1371\/journal.pone.0016557","volume":"6","author":"A Hayward","year":"2011","unstructured":"Hayward, A. & Gillooly, J. F. The cost of sex: quantifying energetic investment in gamete production by males and females. PLoS ONE 6, 16557 (2011).","journal-title":"PLoS ONE"},{"key":"4800_CR51","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.cbpc.2008.07.005","volume":"149","author":"H Yang","year":"2009","unstructured":"Yang, H. & Tiersch, T. R. Current status of sperm cryopreservation in biomedical research fish models: Zebrafish, medaka, and Xiphophorus. Comp. Biochem. Physiol. Part C. Toxicol. Pharmacol. 149, 224\u2013232 (2009).","journal-title":"Comp. Biochem. Physiol. Part C. Toxicol. Pharmacol."},{"key":"4800_CR52","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1038\/nature12111","volume":"496","author":"K Howe","year":"2013","unstructured":"Howe, K. et al. The zebrafish reference genome sequence and its relationship to the human genome. Nat 496, 498\u2013503 (2013).","journal-title":"Nat"},{"key":"4800_CR53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-019-50169-1","volume":"9","author":"Z Marinovi\u0107","year":"2019","unstructured":"Marinovi\u0107, Z. et al. Preservation of zebrafish genetic resources through testis cryopreservation and spermatogonia transplantation. Sci. Rep. 9, 1\u201310 (2019).","journal-title":"Sci. Rep."},{"key":"4800_CR54","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s13205-022-03424-8","volume":"13","author":"SK Majhi","year":"2023","unstructured":"Majhi, S. K. Generation of surrogate goldfish Carassius auratus progeny from common carp Cyprinus carpio parents. 3 Biotech 13, 1\u201314 (2023).","journal-title":"3 Biotech"},{"key":"4800_CR55","doi-asserted-by":"publisher","first-page":"736977","DOI":"10.1016\/j.aquaculture.2021.736977","volume":"543","author":"Y Ren","year":"2021","unstructured":"Ren, Y. et al. Production of donor-derived offsprings by allogeneic transplantation of oogonia in the adult Japanese flounder (Paralichthys olivaceus). Aquaculture 543, 736977 (2021).","journal-title":"Aquaculture"},{"key":"4800_CR56","first-page":"691","volume":"50","author":"S Maegawa","year":"2002","unstructured":"Maegawa, S. et al. Visualization of primordial germ cells in vivo using GFP-nos1 3\u2019UTR mRNA. Int. J. Dev. Biol. 50, 691\u2013700 (2002).","journal-title":"Int. J. Dev. Biol."},{"key":"4800_CR57","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.theriogenology.2011.01.031","volume":"76","author":"RP Panda","year":"2011","unstructured":"Panda, R. P., Barman, H. K. & Mohapatra, C. Isolation of enriched carp spermatogonial stem cells from Labeo rohita testis for in vitro propagation. Theriogenology 76, 241\u2013251 (2011).","journal-title":"Theriogenology"},{"key":"4800_CR58","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1038\/nprot.2010.165","volume":"6","author":"J Sullivan-Brown","year":"2011","unstructured":"Sullivan-Brown, J., Bisher, M. & Burdine, R. Embedding, serial sectioning and staining of zebrafish embryos using JB-4 resin. Nat. Protoc. 6, 46\u201355 (2011).","journal-title":"Nat. Protoc."}],"container-title":["Communications Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s42003-023-04800-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-023-04800-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-023-04800-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T09:06:50Z","timestamp":1681463210000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s42003-023-04800-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,14]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["4800"],"URL":"https:\/\/doi.org\/10.1038\/s42003-023-04800-7","relation":{},"ISSN":["2399-3642"],"issn-type":[{"value":"2399-3642","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,14]]},"assertion":[{"value":"28 December 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 April 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 April 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The study was conducted at the Faculty of Fisheries and Protection of Waters (FFPW), the University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic. The facility has the competence to perform experiments on animals (Act no. 246\/1992 Coll., ref. number 16OZ19179\/2016\u201317214). The Institutional Animal Care and Use Committee of the FFPW approved the methodological protocol of the current study according to the law on the protection of animals against cruelty (reference number: MSMT-6406\/2019\u20132). The study did not involve endangered or protected species. Authors of the study (R.F. and M.P.) possess the Certificate of Professional Competence for designing experiments and experimental projects under Section 15d (3) of Act no. 246\/1992 Coll. on the Protection of Animals Against Cruelty.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"412"}}