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The tandem multigene family<jats:italic>Sdic<\/jats:italic>is specific to<jats:italic>Drosophila melanogaster<\/jats:italic>and has been annotated across multiple reference-quality genome assemblies. Here we show the existence of a positive correlation between<jats:italic>Sdic<\/jats:italic>copy number and total<jats:italic>e<\/jats:italic>xpression, plus vast intrastrain differences in mRNA abundance among paralogs, using RNA-sequencing from testis of four strains with variable paralog composition. Single cell and nucleus RNA-sequencing data expose paralog expression differentiation in meiotic cell types within testis from third instar larva and adults. Additional RNA-sequencing across synthetic strains only differing in their<jats:italic>Y<\/jats:italic>chromosomes reveal a tissue-dependent<jats:italic>trans-<\/jats:italic>regulatory effect on<jats:italic>Sdic<\/jats:italic>: upregulation in testis and downregulation in male accessory gland. By leveraging paralog-specific expression information from tissue- and cell-specific data, our results elucidate the intraspecific functional diversification of a recently expanded tandem gene family.<\/jats:p>","DOI":"10.1038\/s42003-023-05427-4","type":"journal-article","created":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T08:02:48Z","timestamp":1697788968000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Paralog transcriptional differentiation in the D. melanogaster-specific gene family Sdic across populations and spermatogenesis stages"],"prefix":"10.1038","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1025-8772","authenticated-orcid":false,"given":"Bryan D.","family":"Clifton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imtiyaz","family":"Hariyani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashlyn","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangning","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvin","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-3129","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"Ranz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,10,20]]},"reference":[{"key":"5427_CR1","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1104\/pp.114.242826","volume":"166","author":"M Jugulam","year":"2014","unstructured":"Jugulam, M. et al. 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