{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T12:55:49Z","timestamp":1793019349438,"version":"4.5.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Commun Biol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Light serves as a crucial external zeitgeber for maintaining and restoring physiological homeostasis in most organisms. Disrupting of light rhythms often leads to abnormal immune function, characterized by excessive inflammatory responses. However, the underlying regulatory mechanisms behind this phenomenon remain unclear. To address this concern, we use in vivo imaging to establish inflammation models in zebrafish, allowing us to investigate the effects and underlying mechanisms of light disruption on neutrophil recruitment. Our findings reveal that under sustained light conditions (LL), neutrophil recruitment in response to caudal fin injury and otic vesicle inflammation is significantly increased. This is accompanied by elevated levels of histone (H3K18) lactylation and reactive oxygen species (ROS) content. Through ChIP-sequencing and ChIP\u2012qPCR analysis, we discover that H3K18 lactylation regulates the transcriptional activation of the\n                    <jats:italic>duox<\/jats:italic>\n                    gene, leading to ROS production. In turn, ROS further promote H3K18 lactylation, forming a positive feedback loop. This loop, driven by H3K18 lactylation-ROS, ultimately results in the over recruitment of neutrophils to inflammatory sites in LL conditions. Collectively, our study provides evidence of a mutual loop between histone lactylation and ROS, exacerbating neutrophil recruitment in light disorder conditions, emphasizing the significance of maintaining a proper light-dark cycle to optimize immune function.\n                  <\/jats:p>","DOI":"10.1038\/s42003-024-06543-5","type":"journal-article","created":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T08:01:54Z","timestamp":1721462514000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Histone lactylation-ROS loop contributes to light exposure-exacerbated neutrophil recruitment in zebrafish"],"prefix":"10.1038","volume":"7","author":[{"given":"Cheng-zeng","family":"Qiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao-yi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9893-743X","authenticated-orcid":false,"given":"Zong-jun","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2119-7995","authenticated-orcid":false,"given":"Da-long","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,20]]},"reference":[{"key":"6543_CR1","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.jconrel.2019.10.005","volume":"313","author":"JP Garcia-Lopez","year":"2019","unstructured":"Garcia-Lopez, J. P., Vilos, C. & Feijoo, C. G. Zebrafish, a model to develop nanotherapeutics that control neutrophils response during inflammation. J. Control Rel. 313, 14\u201323 (2019).","journal-title":"J. Control Rel."},{"key":"6543_CR2","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1038\/nri3399","volume":"13","author":"E Kolaczkowska","year":"2013","unstructured":"Kolaczkowska, E. & Kubes, P. Neutrophil recruitment and function in health and inflammation. Nat. Rev. Immunol. 13, 159\u2013175 (2013).","journal-title":"Nat. Rev. Immunol."},{"key":"6543_CR3","doi-asserted-by":"publisher","first-page":"3567","DOI":"10.1111\/all.15505","volume":"77","author":"A Ozcan","year":"2022","unstructured":"Ozcan, A. & Boyman, O. Mechanisms regulating neutrophil responses in immunity, allergy, and autoimmunity. Allergy 77, 3567\u20133583 (2022).","journal-title":"Allergy"},{"key":"6543_CR4","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1186\/s13045-021-01187-y","volume":"14","author":"S Xiong","year":"2021","unstructured":"Xiong, S., Dong, L. & Cheng, L. Neutrophils in cancer carcinogenesis and metastasis. J. Hematol. Oncol. 14, 173 (2021).","journal-title":"J. Hematol. Oncol."},{"key":"6543_CR5","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1038\/nri.2016.49","volume":"16","author":"S de Oliveira","year":"2016","unstructured":"de Oliveira, S., Rosowski, E. E. & Huttenlocher, A. Neutrophil migration in infection and wound repair: going forward in reverse. Nat. Rev. Immunol. 16, 378\u2013391 (2016).","journal-title":"Nat. Rev. Immunol."},{"key":"6543_CR6","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1038\/s41392-022-00899-y","volume":"7","author":"F Fagiani","year":"2022","unstructured":"Fagiani, F. et al. Molecular regulations of circadian rhythm and implications for physiology and diseases. Signal Transduct. Target Ther. 7, 41 (2022).","journal-title":"Signal Transduct. Target Ther."},{"key":"6543_CR7","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1111\/brv.12678","volume":"96","author":"VV Krylov","year":"2021","unstructured":"Krylov, V. V. et al. Circadian rhythms in zebrafish (Danio rerio) behaviour and the sources of their variability. Biol. Rev. Camb. Philos. Soc. 96, 785\u2013797 (2021).","journal-title":"Biol. Rev. Camb. Philos. Soc."},{"key":"6543_CR8","doi-asserted-by":"publisher","first-page":"1485","DOI":"10.1016\/j.febslet.2011.04.007","volume":"585","author":"G Vatine","year":"2011","unstructured":"Vatine, G. et al. It\u2019s time to swim! Zebrafish and the circadian clock. FEBS Lett. 585, 1485\u20131494 (2011).","journal-title":"FEBS Lett."},{"key":"6543_CR9","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5753","volume":"5","author":"JA Haspel","year":"2014","unstructured":"Haspel, J. A. et al. Circadian rhythm reprogramming during lung inflammation. Nat. Commun. 5, 4753 (2014).","journal-title":"Nat. Commun."},{"key":"6543_CR10","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113179","volume":"42","author":"AQ Chen","year":"2023","unstructured":"Chen, A. Q. et al. Circadian clock1a coordinates neutrophil recruitment via nfe212a\/duox-reactive oxygen species pathway in zebrafish. Cell Rep. 42, 113179 (2023).","journal-title":"Cell Rep."},{"key":"6543_CR11","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1038\/s41586-019-1678-1","volume":"574","author":"D Zhang","year":"2019","unstructured":"Zhang, D. et al. Metabolic regulation of gene expression by histone lactylation. Nature 574, 575\u2013580 (2019).","journal-title":"Nature"},{"key":"6543_CR12","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02308-z","volume":"22","author":"J Yu","year":"2021","unstructured":"Yu, J. et al. Histone lactylation drives oncogenesis by facilitating m(6)A reader protein YTHDF2 expression in ocular melanoma. Genome Biol. 22, 85 (2021).","journal-title":"Genome Biol."},{"key":"6543_CR13","doi-asserted-by":"crossref","unstructured":"Xu, X., et al., Metabolic reprogramming and epigenetic modifications in cancer: from the impacts and mechanisms to the treatment potential. Exp. Mol. Med., 55, 1357\u20131370 (2023).","DOI":"10.1038\/s12276-023-01020-1"},{"key":"6543_CR14","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1038\/s41418-021-00841-9","volume":"29","author":"K Yang","year":"2022","unstructured":"Yang, K. et al. Lactate promotes macrophage HMGB1 lactylation, acetylation, and exosomal release in polymicrobial sepsis. Cell Death Differ. 29, 133\u2013146 (2022).","journal-title":"Cell Death Differ."},{"key":"6543_CR15","doi-asserted-by":"publisher","first-page":"893","DOI":"10.1161\/CIRCRESAHA.122.320488","volume":"131","author":"N Wang","year":"2022","unstructured":"Wang, N. et al. Histone Lactylation Boosts Reparative Gene Activation Post-Myocardial Infarction. Circ. Res. 131, 893\u2013908 (2022).","journal-title":"Circ. Res."},{"key":"6543_CR16","doi-asserted-by":"publisher","first-page":"30628","DOI":"10.1073\/pnas.2009778117","volume":"117","author":"RA Irizarry-Caro","year":"2020","unstructured":"Irizarry-Caro, R. A. et al. TLR signaling adapter BCAP regulates inflammatory to reparatory macrophage transition by promoting histone lactylation. Proc. Natl Acad. Sci. USA 117, 30628\u201330638 (2020).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"6543_CR17","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1016\/j.it.2022.03.011","volume":"43","author":"A Stream","year":"2022","unstructured":"Stream, A. & Madigan, C. A. Zebrafish: an underutilized tool for discovery in host\u2013microbe interactions. Trends Immunol. 43, 426\u2013437 (2022).","journal-title":"Trends Immunol."},{"key":"6543_CR18","doi-asserted-by":"publisher","first-page":"2531","DOI":"10.7150\/thno.81332","volume":"13","author":"AM Oprisoreanu","year":"2023","unstructured":"Oprisoreanu, A. M. et al. Drug screening in zebrafish larvae reveals inflammation-related modulators of secondary damage after spinal cord injury in mice. Theranostics 13, 2531\u20132551 (2023).","journal-title":"Theranostics"},{"key":"6543_CR19","doi-asserted-by":"publisher","first-page":"eabg3505","DOI":"10.1126\/sciadv.abg3505","volume":"7","author":"S Dichtl","year":"2021","unstructured":"Dichtl, S. et al. Lactate and IL6 define separable paths of inflammatory metabolic adaptation. Sci. Adv. 7, eabg3505 (2021).","journal-title":"Sci. Adv."},{"key":"6543_CR20","doi-asserted-by":"publisher","first-page":"101554","DOI":"10.1016\/j.arr.2021.101554","volume":"74","author":"AD Shkodina","year":"2022","unstructured":"Shkodina, A. D. et al. Roles of clock genes in the pathogenesis of Parkinson\u2019s disease. Ageing Res. Rev. 74, 101554 (2022).","journal-title":"Ageing Res. Rev."},{"key":"6543_CR21","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1038\/s41582-021-00577-7","volume":"18","author":"M Nassan","year":"2022","unstructured":"Nassan, M. & Videnovic, A. Circadian rhythms in neurodegenerative disorders. Nat. Rev. Neurol. 18, 7\u201324 (2022).","journal-title":"Nat. Rev. Neurol."},{"key":"6543_CR22","doi-asserted-by":"publisher","first-page":"828","DOI":"10.1136\/annrheumdis-2020-219515","volume":"80","author":"H Morris","year":"2021","unstructured":"Morris, H. et al. Tissue physiology revolving around the clock: circadian rhythms as exemplified by the intervertebral disc. Ann. Rheum. Dis. 80, 828\u2013839 (2021).","journal-title":"Ann. Rheum. Dis."},{"key":"6543_CR23","doi-asserted-by":"publisher","first-page":"3806","DOI":"10.1158\/0008-5472.CAN-19-0566","volume":"79","author":"AA Shafi","year":"2019","unstructured":"Shafi, A. A. & Knudsen, K. E. Cancer and the Circadian Clock. Cancer Res. 79, 3806\u20133814 (2019).","journal-title":"Cancer Res."},{"key":"6543_CR24","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/j.tins.2022.03.010","volume":"45","author":"F Peng","year":"2022","unstructured":"Peng, F. et al. Circadian clock, diurnal glucose metabolic rhythm, and dawn phenomenon. Trends Neurosci. 45, 471\u2013482 (2022).","journal-title":"Trends Neurosci."},{"key":"6543_CR25","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.1016\/j.tips.2022.09.004","volume":"43","author":"A Giri","year":"2022","unstructured":"Giri, A., Rahman, I. & Sundar, I. K. Circadian clock-based therapeutics in chronic pulmonary diseases. Trends Pharm. Sci. 43, 1014\u20131029 (2022).","journal-title":"Trends Pharm. Sci."},{"key":"6543_CR26","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1038\/s41596-022-00746-y","volume":"18","author":"W Wang","year":"2023","unstructured":"Wang, W. et al. Desynchronizing the sleep-wake cycle from circadian timing to assess their separate contributions to physiology and behaviour and to estimate intrinsic circadian period. Nat. Protoc. 18, 579\u2013603 (2023).","journal-title":"Nat. Protoc."},{"key":"6543_CR27","doi-asserted-by":"publisher","first-page":"2201","DOI":"10.1016\/j.neuron.2023.04.016","volume":"111","author":"L Xie","year":"2023","unstructured":"Xie, L. et al. Cholecystokinin neurons in mouse suprachiasmatic nucleus regulate the robustness of circadian clock. Neuron 111, 2201\u20132217 e4 (2023).","journal-title":"Neuron"},{"key":"6543_CR28","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1007\/s00281-021-00899-0","volume":"44","author":"WH Walker 2nd","year":"2022","unstructured":"Walker, W. H. 2nd et al. Light at night disrupts biological clocks, calendars, and immune function. Semin. Immunopathol. 44, 165\u2013173 (2022).","journal-title":"Semin. Immunopathol."},{"key":"6543_CR29","doi-asserted-by":"publisher","first-page":"804","DOI":"10.1007\/s00134-012-2494-3","volume":"38","author":"AC Verceles","year":"2012","unstructured":"Verceles, A. C. et al. Circadian rhythm disruption in severe sepsis: the effect of ambient light on urinary 6-sulfatoxymelatonin secretion. Intensive Care Med. 38, 804\u2013810 (2012).","journal-title":"Intensive Care Med."},{"key":"6543_CR30","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1016\/j.phrs.2017.08.017","volume":"125","author":"Y Li","year":"2017","unstructured":"Li, Y. et al. Zebrafish: A promising in vivo model for assessing the delivery of natural products, fluorescence dyes and drugs across the blood-brain barrier. Pharm. Res. 125, 246\u2013257 (2017).","journal-title":"Pharm. Res."},{"key":"6543_CR31","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-023-02931-y","volume":"24","author":"X Wang","year":"2023","unstructured":"Wang, X. et al. YY1 lactylation in microglia promotes angiogenesis through transcription activation-mediated upregulation of FGF2. Genome Biol. 24, 87 (2023).","journal-title":"Genome Biol."},{"key":"6543_CR32","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adg4993","volume":"9","author":"M Jia","year":"2023","unstructured":"Jia, M. et al. ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase activity by its lactylation. Sci. Adv. 9, eadg4993 (2023).","journal-title":"Sci. Adv."},{"key":"6543_CR33","doi-asserted-by":"publisher","first-page":"8037","DOI":"10.1093\/nar\/gkab607","volume":"49","author":"Y Xiao","year":"2021","unstructured":"Xiao, Y. et al. HBO1 is a versatile histone acyltransferase critical for promoter histone acylations. Nucleic Acids Res. 49, 8037\u20138059 (2021).","journal-title":"Nucleic Acids Res."},{"key":"6543_CR34","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-022-02775-y","volume":"23","author":"E Galle","year":"2022","unstructured":"Galle, E. et al. H3K18 lactylation marks tissue-specific active enhancers. Genome Biol. 23, 207 (2022).","journal-title":"Genome Biol."},{"key":"6543_CR35","doi-asserted-by":"publisher","first-page":"5259","DOI":"10.1039\/C9CC01747J","volume":"55","author":"AK Sharma","year":"2019","unstructured":"Sharma, A. K., Singh, H. & Chakrapani, H. Photocontrolled endogenous reactive oxygen species (ROS) generation. Chem. Commun. 55, 5259\u20135262 (2019).","journal-title":"Chem. Commun."},{"key":"6543_CR36","doi-asserted-by":"publisher","first-page":"636954","DOI":"10.3389\/fimmu.2021.636954","volume":"12","author":"D Domer","year":"2021","unstructured":"Domer, D. et al. Neutrophil Extracellular Traps Activate Proinflammatory Functions of Human Neutrophils. Front. Immunol. 12, 636954 (2021).","journal-title":"Front. Immunol."},{"key":"6543_CR37","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-022-03893-6","volume":"26","author":"T Pan","year":"2022","unstructured":"Pan, T. et al. Immune effects of PI3K\/Akt\/HIF-1alpha-regulated glycolysis in polymorphonuclear neutrophils during sepsis. Crit. Care 26, 29 (2022).","journal-title":"Crit. Care"},{"key":"6543_CR38","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1530\/JOE-15-0329","volume":"227","author":"DL Ren","year":"2015","unstructured":"Ren, D. L. et al. Exogenous melatonin inhibits neutrophil migration through suppression of ERK activation. J. Endocrinol. 227, 49\u201360 (2015).","journal-title":"J. Endocrinol."},{"key":"6543_CR39","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/s13045-022-01238-y","volume":"15","author":"L Zhou","year":"2022","unstructured":"Zhou, L. et al. Circadian rhythms and cancers: the intrinsic links and therapeutic potentials. J. Hematol. Oncol. 15, 21 (2022).","journal-title":"J. Hematol. Oncol."}],"container-title":["Communications Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s42003-024-06543-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-024-06543-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-024-06543-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T08:03:26Z","timestamp":1721462606000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s42003-024-06543-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,20]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["6543"],"URL":"https:\/\/doi.org\/10.1038\/s42003-024-06543-5","relation":{},"ISSN":["2399-3642"],"issn-type":[{"value":"2399-3642","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,20]]},"assertion":[{"value":"4 January 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 July 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 July 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"887"}}