{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T18:30:37Z","timestamp":1782757837654,"version":"3.54.5"},"reference-count":66,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:00:00Z","timestamp":1729728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:00:00Z","timestamp":1729728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100010024","name":"Hungarian Science Foundation","doi-asserted-by":"publisher","award":["K132155"],"award-info":[{"award-number":["K132155"]}],"id":[{"id":"10.13039\/501100010024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Commun Biol"],"DOI":"10.1038\/s42003-024-07090-9","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T19:02:33Z","timestamp":1729796553000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Versatile gamma-tubulin complexes contribute to the dynamic organization of MTOCs during Drosophila spermatogenesis"],"prefix":"10.1038","volume":"7","author":[{"given":"Elham","family":"Alzyoud","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00f3ra","family":"N\u00e9meth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0420-8226","authenticated-orcid":false,"given":"Viktor","family":"Vedelek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Titanilla","family":"Sz\u00f6gi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikt\u00f3ria Petra","family":"T\u00f3th","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nika","family":"Krecsmarik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1268-760X","authenticated-orcid":false,"given":"Edit","family":"\u00c1brah\u00e1m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2067-0832","authenticated-orcid":false,"given":"Zolt\u00e1n","family":"Lipinszki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4040-4184","authenticated-orcid":false,"given":"Rita","family":"Sinka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,10,24]]},"reference":[{"key":"7090_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/cells7090121","volume":"7","author":"M Tillery","year":"2018","unstructured":"Tillery, M., Blake-Hedges, C., Zheng, Y., Buchwalter, R. & Megraw, T. Centrosomal and non-centrosomal microtubule-organizing centers (MTOCs) in Drosophila melanogaster. Cells 7, 1\u201344 (2018).","journal-title":"Cells"},{"key":"7090_CR2","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1146\/annurev.cellbio.13.1.83","volume":"13","author":"A Desai","year":"1997","unstructured":"Desai, A. & Mitchison, T. J. Microtubule polymerization dynamics. Annu. Rev. Cell Dev. Biol. 13, 83\u2013117 (1997).","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"7090_CR3","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1038\/35014058","volume":"2","author":"M Moritz","year":"2000","unstructured":"Moritz, M., Braunfeld, M. B., Gu\u00e9nebaut, V., Heuser, J. & Agard, D. A. Structure of the \u03b3-tubulin ring complex: a template for microtubule nucleation. Nat. Cell Biol. 2, 365\u2013370 (2000).","journal-title":"Nat. Cell Biol."},{"key":"7090_CR4","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1038\/378578a0","volume":"378","author":"Y Zheng","year":"1995","unstructured":"Zheng, Y., Wong, M. L., Alberts, B. & Mitchison, T. Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex. Nature 378, 578\u2013583 (1995).","journal-title":"Nature"},{"key":"7090_CR5","doi-asserted-by":"publisher","first-page":"1622","DOI":"10.12688\/f1000research.11652.1","volume":"6","author":"AM Fry","year":"2017","unstructured":"Fry, A. M., Sampson, J., Shak, C. & Shackleton, S. Recent advances in pericentriolar material organization: ordered layers and scaffolding gels. F1000Research 6, 1622 (2017).","journal-title":"F1000Research"},{"key":"7090_CR6","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1038\/nrm3209","volume":"12","author":"JM Kollman","year":"2011","unstructured":"Kollman, J. M., Merdes, A., Mourey, L. & Agard, D. A. Microtubule nucleation by \u03b3-tubulin complexes. Nat. Rev. Mol. Cell Biol. 12, 709\u2013721 (2011).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"7090_CR7","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1083\/jcb.144.4.721","volume":"144","author":"K Oegema","year":"1999","unstructured":"Oegema, K. et al. Characterization of two related Drosophila \u03b3-tubulin complexes that differ in their ability to nucleate microtubules. J. Cell Biol. 144, 721\u2013733 (1999).","journal-title":"J. Cell Biol."},{"key":"7090_CR8","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1083\/jcb.151.7.1513","volume":"151","author":"RN Gunawardane","year":"2000","unstructured":"Gunawardane, R. N. et al. Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits. J. Cell Biol. 151, 1513\u20131524 (2000).","journal-title":"J. Cell Biol."},{"key":"7090_CR9","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1083\/jcb.200511071","volume":"172","author":"C V\u00e9rollet","year":"2006","unstructured":"V\u00e9rollet, C. et al. Drosophila melanogaster gamma-TuRC is dispensable for targeting gamma-tubulin to the centrosome and microtubule nucleation. J. Cell Biol. 172, 517\u2013528 (2006).","journal-title":"J. Cell Biol."},{"key":"7090_CR10","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1042\/EBC20180028","volume":"62","author":"CA Tovey","year":"2018","unstructured":"Tovey, C. A. & Conduit, P. T. Microtubule nucleation by \u03b3-tubulin complexes and beyond. Essays Biochem. 62, 765\u2013780 (2018).","journal-title":"Essays Biochem."},{"key":"7090_CR11","doi-asserted-by":"publisher","first-page":"3337","DOI":"10.1091\/mbc.e13-05-0253","volume":"24","author":"DK Dhani","year":"2013","unstructured":"Dhani, D. K. et al. Mzt1\/Tam4, a fission yeast MOZART1 homologue, is an essential component of the \u03b3-tubulin complex and directly interacts with GCP3Alp6. Mol. Biol. Cell 24, 3337\u20133349 (2013).","journal-title":"Mol. Biol. Cell"},{"key":"7090_CR12","doi-asserted-by":"publisher","first-page":"2894","DOI":"10.1091\/mbc.e13-05-0235","volume":"24","author":"H Masuda","year":"2013","unstructured":"Masuda, H., Mori, R., Yukawa, M. & Toda, T. Fission yeast MOZART1\/Mzt1 is an essential \u03b3-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly. Mol. Biol. Cell 24, 2894\u20132906 (2013).","journal-title":"Mol. Biol. Cell"},{"key":"7090_CR13","doi-asserted-by":"publisher","first-page":"2314","DOI":"10.1016\/j.cub.2018.05.044","volume":"28","author":"CA Tovey","year":"2018","unstructured":"Tovey, C. A. et al. \u03b3-TuRC heterogeneity revealed by analysis of Mozart1. Curr. Biol. CB 28, 2314\u20132323.e6 (2018).","journal-title":"Curr. Biol. CB"},{"key":"7090_CR14","doi-asserted-by":"publisher","first-page":"727264","DOI":"10.3389\/fcell.2021.727264","volume":"9","author":"E Alzyoud","year":"2021","unstructured":"Alzyoud, E., Vedelek, V., R\u00e9thi-Nagy, Z., Lipinszki, Z. & Sinka, R. Microtubule organizing centers contain testis-specific \u03b3-TuRC proteins in spermatids of drosophila. Front. Cell Dev. Biol. 9, 727264 (2021).","journal-title":"Front. Cell Dev. Biol."},{"key":"7090_CR15","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/S0022-5320(75)80089-X","volume":"53","author":"KT Tokuyasu","year":"1975","unstructured":"Tokuyasu, K. T. Dynamics of spermiogenesis in Drosophila melanogaster. VI. Significance of \u2018onion\u2019 nebenkern formation. J. Ultrastruct. Res. 53, 93\u2013112 (1975).","journal-title":"J. Ultrastruct. Res."},{"key":"7090_CR16","first-page":"S30","volume":"83","author":"P Avasthi","year":"2012","unstructured":"Avasthi, P. & Marshall, W. F. Stages of ciliogenesis and regulation of ciliary length. Differ. Res. Biol. Divers. 83, S30\u2013S42 (2012).","journal-title":"Differ. Res. Biol. Divers."},{"key":"7090_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pgen.1007987","volume":"15","author":"B Laurinyecz","year":"2019","unstructured":"Laurinyecz, B. et al. Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm. PLoS Genet. 15, 1\u201324 (2019).","journal-title":"PLoS Genet."},{"key":"7090_CR18","doi-asserted-by":"publisher","first-page":"1928","DOI":"10.1016\/j.cub.2017.05.090","volume":"27","author":"JV Chen","year":"2017","unstructured":"Chen, J. V., Buchwalter, R. A., Kao, L.-R. & Megraw, T. L. A splice variant of centrosomin converts mitochondria to microtubule-organizing centers. Curr. Biol. CB 27, 1928\u20131940.e6 (2017).","journal-title":"Curr. Biol. CB"},{"key":"7090_CR19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-018-5085-z","volume":"19","author":"V Vedelek","year":"2018","unstructured":"Vedelek, V. et al. Analysis of Drosophila melanogaster testis transcriptome 06 Biological Sciences 0604 Genetics. BMC Genom. 19, 1\u201319 (2018).","journal-title":"BMC Genom."},{"key":"7090_CR20","doi-asserted-by":"publisher","first-page":"2171","DOI":"10.1016\/j.jprot.2010.09.002","volume":"73","author":"ER Wasbrough","year":"2010","unstructured":"Wasbrough, E. R. et al. The Drosophila melanogaster sperm proteome-II (DmSP-II). J. Proteom. 73, 2171\u20132185 (2010).","journal-title":"J. Proteom."},{"key":"7090_CR21","doi-asserted-by":"publisher","first-page":"3126","DOI":"10.1101\/gad.182800","volume":"14","author":"V Barbosa","year":"2000","unstructured":"Barbosa, V., Yamamoto, R. R., Henderson, D. S. & Glover, D. M. Mutation of a Drosophila gamma tubulin ring complex subunit encoded by discs degenerate-4 differentially disrupts centrosomal protein localization. Genes Dev. 14, 3126\u20133139 (2000).","journal-title":"Genes Dev."},{"key":"7090_CR22","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1091\/mbc.e05-08-0722","volume":"17","author":"N Colombi\u00e9","year":"2006","unstructured":"Colombi\u00e9, N. et al. The Drosophila gamma-tubulin small complex subunit Dgrip84 is required for structural and functional integrity of the spindle apparatus. Mol. Biol. Cell 17, 272\u2013282 (2006).","journal-title":"Mol. Biol. Cell"},{"key":"7090_CR23","doi-asserted-by":"publisher","first-page":"3963","DOI":"10.1242\/dev.02570","volume":"133","author":"N Vogt","year":"2006","unstructured":"Vogt, N., Koch, I., Schwarz, H., Schnorrer, F. & N\u00fcsslein-Volhard, C. The \u03b3TuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline. Development 133, 3963\u20133972 (2006).","journal-title":"Development"},{"key":"7090_CR24","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1016\/j.ejcb.2011.07.007","volume":"90","author":"F B\u00e4renz","year":"2011","unstructured":"B\u00e4renz, F., Mayilo, D. & Gruss, O. J. Centriolar satellites: busy orbits around the centrosome. Eur. J. Cell Biol. 90, 983\u2013989 (2011).","journal-title":"Eur. J. Cell Biol."},{"key":"7090_CR25","doi-asserted-by":"publisher","first-page":"2395","DOI":"10.1016\/j.cub.2020.04.036","volume":"30","author":"H Gupta","year":"2020","unstructured":"Gupta, H. et al. SAS-6 association with \u03b3-tubulin ring complex is required for centriole duplication in human cells. Curr. Biol. 30, 2395\u20132403.e4 (2020).","journal-title":"Curr. Biol."},{"key":"7090_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4161\/spmg.21798","volume":"2","author":"L Fabian","year":"2012","unstructured":"Fabian, L. & Brill, J. A. Big things come from little packages Drosophila spermiogenesis. Spermatogenesis 2, 1\u201316 (2012). 3.","journal-title":"Spermatogenesis"},{"key":"7090_CR27","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1242\/jcs.109.4.727","volume":"109","author":"MH Bre","year":"1996","unstructured":"Bre, M. H. et al. Axonemal tubulin polyglycylation probed with two monoclonal antibodies: widespread evolutionary distribution, appearance during spermatozoan maturation and possible function in motility. J. Cell Sci. 109, 727\u2013738 (1996).","journal-title":"J. Cell Sci."},{"key":"7090_CR28","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1016\/j.cub.2011.04.016","volume":"21","author":"T Noguchi","year":"2011","unstructured":"Noguchi, T., Koizumi, M. & Hayashi, S. Sustained elongation of sperm tail promoted by local remodeling of giant mitochondria in Drosophila. Curr. Biol. 21, 805\u2013814 (2011).","journal-title":"Curr. Biol."},{"key":"7090_CR29","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1083\/jcb.200402130","volume":"165","author":"M Martinez-Campos","year":"2004","unstructured":"Martinez-Campos, M., Basto, R., Baker, J., Kernan, M. & Raff, J. W. The Drosophila pericentrin-like protein is essential for cilia\/flagella function, but appears to be dispensable for mitosis. J. Cell Biol. 165, 673\u2013683 (2004).","journal-title":"J. Cell Biol."},{"key":"7090_CR30","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.sbi.2020.08.008","volume":"66","author":"E Zupa","year":"2021","unstructured":"Zupa, E., Liu, P., W\u00fcrtz, M., Schiebel, E. & Pfeffer, S. The structure of the \u03b3-TuRC: a 25-years-old molecular puzzle. Curr. Opin. Struct. Biol. 66, 15\u201321 (2021).","journal-title":"Curr. Opin. Struct. Biol."},{"key":"7090_CR31","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/S0925-4773(01)00566-4","volume":"110","author":"H-M Bourbon","year":"2002","unstructured":"Bourbon, H.-M. et al. A P-insertion screen identifying novel X-linked essential genes in Drosophila. Mech. Dev. 110, 71\u201383 (2002).","journal-title":"Mech. Dev."},{"key":"7090_CR32","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1038\/nature09445","volume":"467","author":"NS Dzhindzhev","year":"2010","unstructured":"Dzhindzhev, N. S. et al. Asterless is a scaffold for the onset of centriole assembly. Nature 467, 714\u2013718 (2010).","journal-title":"Nature"},{"key":"7090_CR33","doi-asserted-by":"publisher","first-page":"E236","DOI":"10.1038\/ncb1002-e236","volume":"4","author":"VJ Allan","year":"2002","unstructured":"Allan, V. J., Thompson, H. M. & McNiven, M. A. Motoring around the Golgi. Nat. Cell Biol. 4, E236\u2013E242 (2002).","journal-title":"Nat. Cell Biol."},{"key":"7090_CR34","doi-asserted-by":"publisher","first-page":"3827","DOI":"10.1016\/j.febslet.2009.09.048","volume":"583","author":"V Kondylis","year":"2009","unstructured":"Kondylis, V. & Rabouille, C. The Golgi apparatus: lessons from Drosophila. FEBS Lett. 583, 3827\u20133838 (2009).","journal-title":"FEBS Lett."},{"key":"7090_CR35","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1083\/jcb.200808018","volume":"183","author":"R. Sinka","year":"2008","unstructured":"Sinka, R., Gillingham, A.K., Kondylis, V., Munro, S. Golgi coiled-coil proteins contain multiple binding sites for rab family G proteins. J. Cell Biol. 183, 607\u2013615 (2008).","journal-title":"J. Cell Biol."},{"key":"7090_CR36","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/j.devcel.2014.10.007","volume":"31","author":"AK Gillingham","year":"2014","unstructured":"Gillingham, A. K., Sinka, R., Torres, I. L., Lilley, K. S. & Munro, S. Toward a comprehensive map of the effectors of rab GTPases. Dev. Cell 31, 358 (2014).","journal-title":"Dev. Cell"},{"key":"7090_CR37","doi-asserted-by":"publisher","DOI":"10.1242\/dev.199644","volume":"148","author":"A Morohoshi","year":"2021","unstructured":"Morohoshi, A. et al. FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice. Development 148, dev199644 (2021).","journal-title":"Development"},{"key":"7090_CR38","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/cells9122684","volume":"9","author":"MG Riparbelli","year":"2020","unstructured":"Riparbelli, M. G., Persico, V. & Callaini, G. The microtubule cytoskeleton during the early drosophila spermiogenesis. Cells 9, 1\u201318 (2020).","journal-title":"Cells"},{"key":"7090_CR39","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1038\/ncb3274","volume":"18","author":"J Fu","year":"2016","unstructured":"Fu, J. et al. Conserved molecular interactions in centriole-to-centrosome conversion. Nat. Cell Biol. 18, 87\u201399 (2016).","journal-title":"Nat. Cell Biol."},{"key":"7090_CR40","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1038\/ncb2527","volume":"14","author":"J Gopalakrishnan","year":"2012","unstructured":"Gopalakrishnan, J. et al. Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment. Nat. Cell Biol. 14, 865\u2013873 (2012).","journal-title":"Nat. Cell Biol."},{"key":"7090_CR41","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12476","volume":"7","author":"BJ Galletta","year":"2016","unstructured":"Galletta, B. J. et al. A centrosome interactome provides insight into organelle assembly and reveals a non-duplication role for Plk4. Nat. Commun. 7, 12476 (2016).","journal-title":"Nat. Commun."},{"key":"7090_CR42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/ncomms1367","volume":"2","author":"J Gopalakrishnan","year":"2011","unstructured":"Gopalakrishnan, J. et al. Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. Nat. Commun. 2, 1\u201311 (2011). 359.","journal-title":"Nat. Commun."},{"key":"7090_CR43","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1016\/j.cell.2011.08.047","volume":"147","author":"KG Guruharsha","year":"2011","unstructured":"Guruharsha, K. G. et al. A protein complex network of Drosophila melanogaster. Cell 147, 690\u2013703 (2011).","journal-title":"Cell"},{"key":"7090_CR44","doi-asserted-by":"publisher","first-page":"2526","DOI":"10.1016\/j.cub.2014.08.061","volume":"24","author":"NS Dzhindzhev","year":"2014","unstructured":"Dzhindzhev, N. S. et al. Plk4 phosphorylates Ana2 to trigger Sas6 recruitment and procentriole formation. Curr. Biol. CB 24, 2526\u20132532 (2014).","journal-title":"Curr. Biol. CB"},{"key":"7090_CR45","doi-asserted-by":"publisher","first-page":"23112","DOI":"10.1074\/jbc.M116.744862","volume":"291","author":"D Farache","year":"2016","unstructured":"Farache, D. et al. Functional analysis of \u00f4-tubulin complex proteins indicates specific lateral association via their N-terminal domains. J. Biol. Chem. 291, 23112\u201323125 (2016).","journal-title":"J. Biol. Chem."},{"key":"7090_CR46","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1083\/jcb.142.3.775","volume":"142","author":"M Moritz","year":"1998","unstructured":"Moritz, M., Zheng, Y., Alberts, B. M. & Oegema, K. Recruitment of the gamma-tubulin ring complex to Drosophila salt-stripped centrosome scaffolds. J. Cell Biol. 142, 775\u2013786 (1998).","journal-title":"J. Cell Biol."},{"key":"7090_CR47","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1042\/bj20011520","volume":"365","author":"E Kurucz","year":"2002","unstructured":"Kurucz, E. et al. Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements. Biochem. J. 365, 527 (2002).","journal-title":"Biochem. J."},{"key":"7090_CR48","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1016\/j.devcel.2007.04.002","volume":"12","author":"A Efimov","year":"2007","unstructured":"Efimov, A. et al. Asymmetric CLASP-dependent nucleation of non-centrosomal microtubules at the trans-Golgi network. Dev. Cell 12, 917\u2013930 (2007).","journal-title":"Dev. Cell"},{"key":"7090_CR49","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.65672","volume":"10","author":"R Becker","year":"2021","unstructured":"Becker, R. et al. Myogenin controls via AKAP6 non-centrosomal microtubule-organizing center formation at the nuclear envelope. eLife 10, e65672 (2021).","journal-title":"eLife"},{"key":"7090_CR50","doi-asserted-by":"publisher","first-page":"790","DOI":"10.1016\/j.devcel.2010.03.015","volume":"18","author":"V Brodu","year":"2010","unstructured":"Brodu, V., Baffet, A. D., Le Droguen, P.-M., Casanova, J. & Guichet, A. A developmentally regulated two-step process generates a noncentrosomal microtubule network in Drosophila tracheal cells. Dev. Cell 18, 790\u2013801 (2010).","journal-title":"Dev. Cell"},{"key":"7090_CR51","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202010020","volume":"220","author":"CA Tovey","year":"2021","unstructured":"Tovey, C. A. et al. Autoinhibition of Cnn binding to \u03b3-TuRCs prevents ectopic microtubule nucleation and cell division defects. J. Cell Biol. 220, e202010020 (2021).","journal-title":"J. Cell Biol."},{"key":"7090_CR52","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.58943","volume":"9","author":"A Mukherjee","year":"2020","unstructured":"Mukherjee, A., Brooks, P. S., Bernard, F., Guichet, A. & Conduit, P. T. Microtubules originate asymmetrically at the somatic golgi and are guided via Kinesin2 to maintain polarity within neurons. eLife 9, e58943 (2020).","journal-title":"eLife"},{"key":"7090_CR53","doi-asserted-by":"publisher","first-page":"1016","DOI":"10.1038\/emboj.2009.47","volume":"28","author":"S Rivero","year":"2009","unstructured":"Rivero, S., Cardenas, J., Bornens, M. & Rios, R. M. Microtubule nucleation at the cis\u2010side of the Golgi apparatus requires AKAP450 and GM130. EMBO J. 28, 1016\u20131028 (2009).","journal-title":"EMBO J."},{"key":"7090_CR54","doi-asserted-by":"publisher","first-page":"1520","DOI":"10.1080\/15548627.2018.1458170","volume":"14","author":"VK Lund","year":"2018","unstructured":"Lund, V. K., Madsen, K. L. & Kjaerulff, O. Drosophila Rab2 controls endosome-lysosome fusion and LAMP delivery to late endosomes. Autophagy 14, 1520\u20131542 (2018).","journal-title":"Autophagy"},{"key":"7090_CR55","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.1242\/bio.018275","volume":"5","author":"K F\u00e1ri","year":"2016","unstructured":"F\u00e1ri, K., Tak\u00e1cs, S., Ung\u00e1r, D. & Sinka, R. The role of acroblast formation during Drosophila spermatogenesis. Biol. Open 5, 1102\u20131110 (2016).","journal-title":"Biol. Open"},{"key":"7090_CR56","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1242\/dev.00325","volume":"130","author":"D Chen","year":"2003","unstructured":"Chen, D. & McKearin, D. M. A discrete transcriptional silencer in the bam gene determines asymmetric division of the Drosophila germline stem cell. Development 130, 1159\u20131170 (2003).","journal-title":"Development"},{"key":"7090_CR57","doi-asserted-by":"publisher","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","volume":"23","author":"MA Larkin","year":"2007","unstructured":"Larkin, M. A. et al. Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947\u20132948 (2007).","journal-title":"Bioinformatics"},{"key":"7090_CR58","doi-asserted-by":"publisher","first-page":"1166","DOI":"10.1093\/bioinformatics\/bts091","volume":"28","author":"K Okonechnikov","year":"2012","unstructured":"Okonechnikov, K., Golosova, O. & Fursov, M. UGENE team Unipro UGENE: a unified bioinformatics toolkit. Bioinformatics 28, 1166\u20131167 (2012).","journal-title":"Bioinformatics"},{"key":"7090_CR59","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4792","volume":"32","author":"EC Meng","year":"2023","unstructured":"Meng, E. C. et al. UCSF ChimeraX: Tools for structure building and analysis. Protein Sci. 32, e4792 (2023).","journal-title":"Protein Sci."},{"key":"7090_CR60","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1038\/nmeth.1318","volume":"6","author":"DG Gibson","year":"2009","unstructured":"Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343\u2013345 (2009).","journal-title":"Nat. Methods"},{"key":"7090_CR61","doi-asserted-by":"crossref","unstructured":"Port, F. & Bullock, S. L. Creating heritable mutations in Drosophila with CRISPR-Cas9. in Methods in Molecular Biology vol. 1478 145\u2013160 (Humana Press, New York, NY, 2016).","DOI":"10.1007\/978-1-4939-6371-3_7"},{"key":"7090_CR62","doi-asserted-by":"crossref","unstructured":"Port, F., Chen, H. M., Lee, T. & Bullock, S. L. Optimized CRISPR\/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc. Natl. Acad. Sci. USA. 111, E2967\u2013E2976 (2014).","DOI":"10.1073\/pnas.1405500111"},{"key":"7090_CR63","unstructured":"White-cooper, H. Spermatogenesis. in Drosophila Cytogenetics Protocols vol. 247 45\u201375 (Humana Press, New York, NY, 2004)."},{"key":"7090_CR64","doi-asserted-by":"publisher","first-page":"1425","DOI":"10.1093\/genetics\/144.4.1425","volume":"144","author":"P James","year":"1996","unstructured":"James, P., Halladay, J. & Craig, E. A. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144, 1425\u20131436 (1996).","journal-title":"Genetics"},{"key":"7090_CR65","doi-asserted-by":"publisher","first-page":"200343","DOI":"10.1098\/rsob.200343","volume":"10","author":"Z Karman","year":"2020","unstructured":"Karman, Z. et al. Novel perspectives of target-binding by the evolutionarily conserved PP4 phosphatase. Open Biol. 10, 200343 (2020).","journal-title":"Open Biol."},{"key":"7090_CR66","doi-asserted-by":"publisher","first-page":"1988","DOI":"10.1002\/2211-5463.13485","volume":"12","author":"Z R\u00e9thi-Nagy","year":"2022","unstructured":"R\u00e9thi-Nagy, Z., \u00c1brah\u00e1m, E. & Lipinszki, Z. GST-IVTT pull-down: a fast and versatile in vitro method for validating and mapping protein-protein interactions. FEBS Open Bio. 12, 1988\u20131995 (2022).","journal-title":"FEBS Open Bio"}],"container-title":["Communications Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s42003-024-07090-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-024-07090-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s42003-024-07090-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T19:02:57Z","timestamp":1729796577000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s42003-024-07090-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,24]]},"references-count":66,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["7090"],"URL":"https:\/\/doi.org\/10.1038\/s42003-024-07090-9","relation":{},"ISSN":["2399-3642"],"issn-type":[{"value":"2399-3642","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,24]]},"assertion":[{"value":"16 January 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 October 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 October 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"1385"}}